DOI: 10.1002/jemt.1070270402. Variations in mitochondrial ultrastructure and dynamics observed by high resolution scanning electron microscopy (HRSEM)

Variations in mitochondrial ultrastructure and dynamics observed by high resolution scanning electron microscopy (HRSEM)

10.1002/jemt.1070270402

Crossref journal-article

Wiley

Microscopy Research and Technique (311)

Abstract

AbstractRat adrenal cortex was processed for high resolution scanning electron microscopy (HRSEM) to confirm tubular cristae, reported by transmission electron microscopy to be present in cortex mitochondria. Mitochondria in several other tissue and cell types were also observed and their ultrastructure confirmed by using three‐dimensional, stereo, high resolution scanning electron microscopy. The mitochondria in rat and human hepatocytes as well as human skin fibroblasts mitochondria proved to be long, up to 46 micrometers and branching, as compared to those in liver which were spherical in shape. Cold adapted brown fat cells were packed with mitochondria, these containing plate or shelf‐like cristae. Branched, rat striated muscle mitochondria were observed to curve around contractile protein filament bundles. The muscle mitochondrial cristae were found to be both tubular and plate‐like, within the same mitochondrion. The ratio of tubular cristae to plate‐like cristae varied considerably between muscle mitochondria. In order to use ultrastructural changes in mitochondria for differential diagnosis, and because 3D reconstruction of mitochondria based on transmission electron microscopy serial sections is severely limited in resolution, it is imperative to first develop a correct understanding of tissue specific, normal mitochondrial ultrastructure based on three‐dimensional, HRSEM methods. © 1994 Wiley‐Liss, Inc.

Bibliography

Lea, P. J., Temkin, R. J., Freeman, K. B., Mitchell, G. A., & Robinson, B. H. (1994). Variations in mitochondrial ultrastructure and dynamics observed by high resolution scanning electron microscopy (HRSEM). Microscopy Research and Technique, 27(4), 269â277. Portico.

Authors 5

Peter J. Lea (first)
Robert J. Temkin (additional)
Karl B. Freeman (additional)
Grant A. Mitchell (additional)
Brain H. Robinson (additional)

References 22 Referenced 36

{'key': 'e_1_2_1_2_1', 'volume-title': 'Die Elementarorganismen und Ihre Beziehung zur den Zellen', 'author': 'Altman R.', 'year': '1890'} / Die Elementarorganismen und Ihre Beziehung zur den Zellen by Altman R. (1890)
10.1083/jcb.107.2.481
{'key': 'e_1_2_1_4_1', 'first-page': '1', 'article-title': 'Ultrastructure of motor end plates and sarcoplasmic components of mouse skeletal muscle fiber as revealed by three dimensional reconstructions from serial sections', 'volume': '1', 'author': 'Andersen‐Cedergren E.', 'year': '1959', 'journal-title': 'J. Ultrastruct. Res. (Suppl.)'} / J. Ultrastruct. Res. (Suppl.) / Ultrastructure of motor end plates and sarcoplasmic components of mouse skeletal muscle fiber as revealed by three dimensional reconstructions from serial sections by Andersen‐Cedergren E. (1959)
{'key': 'e_1_2_1_5_1', 'first-page': '376', 'volume-title': 'Weitere Mitteilungen ueber die Mitochondria', 'author': 'Benda C.', 'year': '1898'} / Weitere Mitteilungen ueber die Mitochondria by Benda C. (1898)
10.1016/S0022-3476(89)80333-6
{'key': 'e_1_2_1_7_1', 'first-page': '263', 'volume-title': 'Paramecium. A Current Survey', 'author': 'Ehret C. S.', 'year': '1974'} / Paramecium. A Current Survey by Ehret C. S. (1974)
10.1016/S0046-8177(81)80121-9
10.1002/jemt.1060180304
10.1002/jemt.1070220207
10.1002/aja.1001840308
{'key': 'e_1_2_1_12_1', 'first-page': '291', 'article-title': 'Investigating the substitution of ethanol with liquid carbon dioxide during critical point drying', 'volume': '83', 'author': 'Lea P. J.', 'year': '1980', 'journal-title': 'Microsc. Acta'} / Microsc. Acta / Investigating the substitution of ethanol with liquid carbon dioxide during critical point drying by Lea P. J. (1980)
10.1083/jcb.107.2.503
10.1007/BF01805595
10.1007/BF00218939
10.1002/ar.1091140304
{'key': 'e_1_2_1_17_1', 'first-page': '1519', 'article-title': 'Working at higher magnification in scanning electron microscopy with secondary and backscattered electrons on metal coated biological specimens and imaging macromolecular cell membrane structures', 'volume': '4', 'author': 'Peters K. R.', 'year': '1985', 'journal-title': 'Scanning Electron Microsc.'} / Scanning Electron Microsc. / Working at higher magnification in scanning electron microscopy with secondary and backscattered electrons on metal coated biological specimens and imaging macromolecular cell membrane structures by Peters K. R. (1985)
10.3109/03009748809098773
10.1002/jmr.300010105
10.1002/jemt.1060180305
{'key': 'e_1_2_1_21_1', 'first-page': '151', 'article-title': 'Mitochondrial tubules in the rat adrenal cortex', 'volume': '103', 'author': 'Wheatly D. N.', 'year': '1968', 'journal-title': 'J. Anat.'} / J. Anat. / Mitochondrial tubules in the rat adrenal cortex by Wheatly D. N. (1968)
10.1002/jemt.1060180306
10.1073/pnas.82.13.4443

Dates

Type	When
Created	20 years, 5 months ago (Feb. 23, 2005, 5:16 p.m.)
Deposited	1 year, 9 months ago (Oct. 24, 2023, 2:15 a.m.)
Indexed	1 month, 3 weeks ago (June 27, 2025, 6:35 a.m.)
Issued	31 years, 5 months ago (March 1, 1994)
Published	31 years, 5 months ago (March 1, 1994)
Published Online	20 years, 6 months ago (Feb. 4, 2005)
Published Print	31 years, 5 months ago (March 1, 1994)

Funders 0

None

BibTeX

@article{Lea_1994, title={Variations in mitochondrial ultrastructure and dynamics observed by high resolution scanning electron microscopy (HRSEM)}, volume={27}, ISSN={1097-0029}, url={http://dx.doi.org/10.1002/jemt.1070270402}, DOI={10.1002/jemt.1070270402}, number={4}, journal={Microscopy Research and Technique}, publisher={Wiley}, author={Lea, Peter J. and Temkin, Robert J. and Freeman, Karl B. and Mitchell, Grant A. and Robinson, Brain H.}, year={1994}, month=mar, pages={269–277} }

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