DOI: 10.1002/jemt.1070260603. Comparison of hexamethyldisilazane (HMDS), Peldri II, and critical‐point drying methods for scanning electron microscopy of biological specimens

Comparison of hexamethyldisilazane (HMDS), Peldri II, and critical‐point drying methods for scanning electron microscopy of biological specimens

10.1002/jemt.1070260603

Crossref journal-article

Wiley

Microscopy Research and Technique (311)

Abstract

AbstractThree different drying methods, critical‐point drying (CPD), Peldri II, and hexamethyldisilazane (HMDS), were compared using representative animal( rat kidney, trachea, duodenum, lung, and red blood cells) and plant( leaves from ten species of monocotyledons and dicotyledons) specimens. All three drying methods produced identical results with animal specimens. Plant specimens showed signs of shrinkage regardless of which drying method was employed. The order of preservation quality from best to worst for leaves was CPD > Peldri II > HMDS, with the CPD method providing substantially better results in all but one case. Postfixation of leaves with osmium tetroxide resulted in poorer preservation in all instances. Peldri II caused complete extraction of leaf cuticular wax, while both both CPD and HMDS showed minimal extraction compared with samples air dried directly from acetone. These results indicate that HMDS provides a time‐saving and inexpensive alternative to CPD for animal specimens. Plant specimens, particularly those containing cells with large central vacuoles, are adequately preserved only with the CPD method. In addition, postfixation with osmium should be avoided when processing plant specimens for scanning electron microscopy. © 1993 Wiley‐Liss, Inc.

Bibliography

Bray, D. F., Bagu, J., & Koegler, P. (1993). Comparison of hexamethyldisilazane (HMDS), Peldri II, and criticalâpoint drying methods for scanning electron microscopy of biological specimens. Microscopy Research and Technique, 26(6), 489â495. Portico.

Authors 3

D. F. Bray (first)
J. Bagu (additional)
P. Koegler (additional)

References 17 Referenced 213

{'key': 'e_1_2_1_2_1', 'first-page': '71', 'volume-title': 'The Science of Biological Specimen Preparation for Microscopy and Microanalysis', 'author': 'Boyde A.', 'year': '1983'} / The Science of Biological Specimen Preparation for Microscopy and Microanalysis by Boyde A. (1983)
10.1016/0034-6667(90)90004-3
10.1111/j.1365-2818.1989.tb02925.x
10.1111/j.1365-2818.1983.tb04166.x
{'key': 'e_1_2_1_6_1', 'first-page': '65B', 'article-title': 'Étude en microscopie électronique a balayage de Campylobacter pylori et des lésions gastroduodénales associées', 'volume': '13', 'author': 'Foliguet B.', 'year': '1989', 'journal-title': 'Gastroenterol. Clin. Biol.'} / Gastroenterol. Clin. Biol. / Étude en microscopie électronique a balayage de Campylobacter pylori et des lésions gastroduodénales associées by Foliguet B. (1989)
{'key': 'e_1_2_1_7_1', 'first-page': '187a', 'article-title': 'Hexamethyldisilazane for rapid scanning electron microscopic examination of implant specimens', 'volume': '66', 'author': 'Giammara B.', 'year': '1987', 'journal-title': 'J. Dental Res.'} / J. Dental Res. / Hexamethyldisilazane for rapid scanning electron microscopic examination of implant specimens by Giammara B. (1987)
{'key': 'e_1_2_1_8_1', 'series-title': 'Electr. Microsc. Sce. Am., Milwaukee', 'first-page': '87', 'volume-title': 'Microscopy: The Key Research Tool', 'author': 'Hardy J. P.', 'year': '1992'} / Microscopy: The Key Research Tool / Electr. Microsc. Sce. Am., Milwaukee by Hardy J. P. (1992)
{'key': 'e_1_2_1_9_1', 'first-page': '131', 'volume-title': 'Introduction to Biological Electron Microscopy', 'author': 'Hayat M. A.', 'year': '1978'} / Introduction to Biological Electron Microscopy by Hayat M. A. (1978)
10.1159/000265435
10.1002/jemt.1060140105
{'key': 'e_1_2_1_12_1', 'first-page': '225a', 'article-title': 'Peldri II—A sublimation method for drying soft biological tissues for scanning electron microscopy', 'volume': '105', 'author': 'Kennedy J. R.', 'year': '1987', 'journal-title': 'J. Cell Biol.'} / J. Cell Biol. / Peldri II—A sublimation method for drying soft biological tissues for scanning electron microscopy by Kennedy J. R. (1987)
10.1002/jemt.1060110205
{'key': 'e_1_2_1_14_1', 'first-page': '164', 'article-title': 'HMDS‐drying—An economical alternative to the critical‐point method', 'volume': '21', 'author': 'Kuhnel V. T.', 'year': '1989', 'journal-title': 'Handchir. Mikrochir. Plast. Chir.'} / Handchir. Mikrochir. Plast. Chir. / HMDS‐drying—An economical alternative to the critical‐point method by Kuhnel V. T. (1989)
10.3109/10520298309066811
{'key': 'e_1_2_1_16_1', 'first-page': '198', 'article-title': 'An evaluation of the usefulness of air‐drying biological samples from tetramethylsilane in preparation for scanning electron microscopy', 'volume': '40', 'author': 'Reville W. J.', 'year': '1991', 'journal-title': 'J. Electron Microsc.'} / J. Electron Microsc. / An evaluation of the usefulness of air‐drying biological samples from tetramethylsilane in preparation for scanning electron microscopy by Reville W. J. (1991)
10.1111/j.1365-2818.1983.tb04164.x
{'key': 'e_1_2_1_18_1', 'first-page': '760', 'volume-title': 'Proceedings of the 50th Annual Meeting of the Electron Microsc. Soc. Am.', 'author': 'Weyda F.', 'year': '1992'} / Proceedings of the 50th Annual Meeting of the Electron Microsc. Soc. Am. by Weyda F. (1992)

Dates

Type	When
Created	20 years, 5 months ago (Feb. 23, 2005, 5:20 p.m.)
Deposited	1 year, 9 months ago (Oct. 24, 2023, 10:05 p.m.)
Indexed	2 weeks, 6 days ago (July 31, 2025, 11:46 p.m.)
Issued	31 years, 8 months ago (Dec. 15, 1993)
Published	31 years, 8 months ago (Dec. 15, 1993)
Published Online	20 years, 6 months ago (Feb. 4, 2005)
Published Print	31 years, 8 months ago (Dec. 15, 1993)

Funders 0

None

BibTeX

@article{Bray_1993, title={Comparison of hexamethyldisilazane (HMDS), Peldri II, and critical‐point drying methods for scanning electron microscopy of biological specimens}, volume={26}, ISSN={1097-0029}, url={http://dx.doi.org/10.1002/jemt.1070260603}, DOI={10.1002/jemt.1070260603}, number={6}, journal={Microscopy Research and Technique}, publisher={Wiley}, author={Bray, D. F. and Bagu, J. and Koegler, P.}, year={1993}, month=dec, pages={489–495} }

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