DOI: 10.1002/jemt.1060190111. Routine cryofixation of plant tissue by propane jet freezing for freeze substitution

Routine cryofixation of plant tissue by propane jet freezing for freeze substitution

10.1002/jemt.1060190111

Crossref journal-article

Wiley

Journal of Electron Microscopy Technique (311)

Abstract

AbstractCryofixation and freeze substitution methods were developed for ultrastructural studies of cells in complex plant tissues. Leaf tissues and root tips of tobacco (Nicotiana tabacum L. var. Maryland Mammoth) were frozen with a RMC MF7200 propane jet freezer and freeze substituted sequentially with tannic acid and osmium tetroxide/uranyl acetate in acetone. High quality preservation was consistently obtained for epidermal and phloem cells of the leaf, and epidermal, cortical, meristematic, and cap cells of the root tip. Leaf mesophyll cells were also often well frozen. Organelles, including nuclei, endoplasmic reticulum, mitochondria, Golgi bodies, and plastids, showed excellent structural integrity and contrast. Most notable is the superior preservation of the cytoskeleton. Our results demonstrate that the propane jet freezer can be used routinely for high quality cryofixation of higher plant cells in certain complex tissues. This could have important implications for the use of cryofixation approach in a wide range of research in plant biology.

Bibliography

Ding, B., Turgeon, R., & Parthasarathy, M. V. (1991). Routine cryofixation of plant tissue by propane jet freezing for freeze substitution. Journal of Electron Microscopy Technique, 19(1), 107â117. Portico.

Authors 3

Biao Ding (first)
Robert Turgeon (additional)
M. V. Parthasarathy (additional)

References 29 Referenced 27

10.1007/BF01276279
{'key': 'e_1_2_1_3_1', 'first-page': '191', 'article-title': 'Cryofixation of tissue surfaces by a propane jet for electron microscopy', 'volume': '9', 'author': 'Burstein N. L.', 'year': '1978', 'journal-title': 'Micron'} / Micron / Cryofixation of tissue surfaces by a propane jet for electron microscopy by Burstein N. L. (1978)
{'key': 'e_1_2_1_4_1', 'first-page': '80', 'article-title': 'High pressure freezing of intact plant tissues. Evaluation and characterization of novel features of the endoplasmic reticulum and associated membrane systems', 'volume': '46', 'author': 'Craig S.', 'year': '1988', 'journal-title': 'Eur. J. Cell Biol.'} / Eur. J. Cell Biol. / High pressure freezing of intact plant tissues. Evaluation and characterization of novel features of the endoplasmic reticulum and associated membrane systems by Craig S. (1988)
{'key': 'e_1_2_1_5_1', 'first-page': '215', 'article-title': 'A copper block method for freezing non‐cryoprotected tissue to produce ice‐crystal‐free regions for electron microscopy', 'volume': '106', 'author': 'Dempsey G. P.', 'year': '1975', 'journal-title': 'J. Microsc.'} / J. Microsc. / A copper block method for freezing non‐cryoprotected tissue to produce ice‐crystal‐free regions for electron microscopy by Dempsey G. P. (1975)
10.1007/BF00395411
{'key': 'e_1_2_1_7_1', 'first-page': '144', 'article-title': 'Ultrastructure of freeze substituted phloem of tobacco leaf', 'volume': '89', 'author': 'Ding B.', 'year': '1989', 'journal-title': 'Plant Physiol.'} / Plant Physiol. / Ultrastructure of freeze substituted phloem of tobacco leaf by Ding B. (1989)
{'key': 'e_1_2_1_8_1', 'first-page': '704', 'volume-title': 'Electron Microscopy 1990. Proceedings of the XIIth International Congress for Electron Microscopy', 'author': 'Ding B.', 'year': '1990'} / Electron Microscopy 1990. Proceedings of the XIIth International Congress for Electron Microscopy by Ding B. (1990)
10.1007/BF00398090
10.1104/pp.56.5.555
10.1002/jemt.1060030206
10.1111/1365-3040.ep11604101 / Plant Cell Environ. / The cells shed by the root cap of Zea : Their origin and some structural and physiological properties by Guinel F. C. (1987)
10.1111/j.1365-2818.1984.tb04650.x
10.1016/0014-4827(72)90183-8
10.1111/j.1365-2818.1989.tb00591.x
10.1007/BF01285037
10.1002/jemt.1060040302
10.1111/j.1365-2818.1978.tb00116.x
10.1007/978-3-642-72815-0_8
10.1007/BF01279752
10.1016/S0074-7696(08)61676-9
10.2307/2444616
10.1111/j.1365-2818.1982.tb00331.x
10.1016/S0022-5320(69)90033-1
10.1007/BF00395066
{'key': 'e_1_2_1_26_1', 'first-page': '253', 'article-title': 'High Pressure freezing comes of age', 'volume': '3', 'author': 'Studer D.', 'year': '1989', 'journal-title': 'Scan Microsc.'} / Scan Microsc. / High Pressure freezing comes of age by Studer D. (1989)
10.1007/BF01276371
10.1083/jcb.99.1.63s
10.1104/pp.76.1.45
10.1007/BF00395069

Dates

Type	When
Created	20 years, 5 months ago (Feb. 24, 2005, 3:22 a.m.)
Deposited	1 year, 9 months ago (Oct. 22, 2023, 5:48 p.m.)
Indexed	1 year, 1 month ago (July 8, 2024, 1:50 a.m.)
Issued	33 years, 11 months ago (Sept. 1, 1991)
Published	33 years, 11 months ago (Sept. 1, 1991)
Published Online	20 years, 6 months ago (Feb. 4, 2005)
Published Print	33 years, 11 months ago (Sept. 1, 1991)

Funders 0

None

BibTeX

@article{Ding_1991, title={Routine cryofixation of plant tissue by propane jet freezing for freeze substitution}, volume={19}, ISSN={1553-0817}, url={http://dx.doi.org/10.1002/jemt.1060190111}, DOI={10.1002/jemt.1060190111}, number={1}, journal={Journal of Electron Microscopy Technique}, publisher={Wiley}, author={Ding, Biao and Turgeon, Robert and Parthasarathy, M. V.}, year={1991}, month=sep, pages={107–117} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2024,
        7,
        8
      ]
    ],
    "date-time": "2024-07-08T05:50:01Z",
    "timestamp": 1720417801079
  },
  "reference-count": 29,
  "publisher": "Wiley",
  "issue": "1",
  "license": [
    {
      "start": {
        "date-parts": [
          [
            2005,
            2,
            4
          ]
        ],
        "date-time": "2005-02-04T00:00:00Z",
        "timestamp": 1107475200000
      },
      "content-version": "vor",
      "delay-in-days": 4905,
      "URL": "http://onlinelibrary.wiley.com/termsAndConditions#vor"
    }
  ],
  "content-domain": {
    "domain": [],
    "crossmark-restriction": false
  },
  "published-print": {
    "date-parts": [
      [
        1991,
        9
      ]
    ]
  },
  "abstract": "<jats:title>Abstract</jats:title><jats:p>Cryofixation and freeze substitution methods were developed for ultrastructural studies of cells in complex plant tissues. Leaf tissues and root tips of tobacco (<jats:italic>Nicotiana tabacum</jats:italic> L. var. Maryland Mammoth) were frozen with a RMC MF7200 propane jet freezer and freeze substituted sequentially with tannic acid and osmium tetroxide/uranyl acetate in acetone. High quality preservation was consistently obtained for epidermal and phloem cells of the leaf, and epidermal, cortical, meristematic, and cap cells of the root tip. Leaf mesophyll cells were also often well frozen. Organelles, including nuclei, endoplasmic reticulum, mitochondria, Golgi bodies, and plastids, showed excellent structural integrity and contrast. Most notable is the superior preservation of the cytoskeleton. Our results demonstrate that the propane jet freezer can be used routinely for high quality cryofixation of higher plant cells in certain complex tissues. This could have important implications for the use of cryofixation approach in a wide range of research in plant biology.</jats:p>",
  "DOI": "10.1002/jemt.1060190111",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2005,
        2,
        24
      ]
    ],
    "date-time": "2005-02-24T08:22:21Z",
    "timestamp": 1109233341000
  },
  "page": "107-117",
  "source": "Crossref",
  "is-referenced-by-count": 27,
  "title": "Routine cryofixation of plant tissue by propane jet freezing for freeze substitution",
  "prefix": "10.1002",
  "volume": "19",
  "author": [
    {
      "given": "Biao",
      "family": "Ding",
      "sequence": "first",
      "affiliation": []
    },
    {
      "given": "Robert",
      "family": "Turgeon",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "M. V.",
      "family": "Parthasarathy",
      "sequence": "additional",
      "affiliation": []
    }
  ],
  "member": "311",
  "published-online": {
    "date-parts": [
      [
        2005,
        2,
        4
      ]
    ]
  },
  "reference": [
    {
      "key": "e_1_2_1_2_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1007/BF01276279"
    },
    {
      "key": "e_1_2_1_3_1",
      "first-page": "191",
      "article-title": "Cryofixation of tissue surfaces by a propane jet for electron microscopy",
      "volume": "9",
      "author": "Burstein N. L.",
      "year": "1978",
      "journal-title": "Micron"
    },
    {
      "key": "e_1_2_1_4_1",
      "first-page": "80",
      "article-title": "High pressure freezing of intact plant tissues. Evaluation and characterization of novel features of the endoplasmic reticulum and associated membrane systems",
      "volume": "46",
      "author": "Craig S.",
      "year": "1988",
      "journal-title": "Eur. J. Cell Biol."
    },
    {
      "key": "e_1_2_1_5_1",
      "first-page": "215",
      "article-title": "A copper block method for freezing non\u2010cryoprotected tissue to produce ice\u2010crystal\u2010free regions for electron microscopy",
      "volume": "106",
      "author": "Dempsey G. P.",
      "year": "1975",
      "journal-title": "J. Microsc."
    },
    {
      "key": "e_1_2_1_6_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1007/BF00395411"
    },
    {
      "key": "e_1_2_1_7_1",
      "first-page": "144",
      "article-title": "Ultrastructure of freeze substituted phloem of tobacco leaf",
      "volume": "89",
      "author": "Ding B.",
      "year": "1989",
      "journal-title": "Plant Physiol."
    },
    {
      "key": "e_1_2_1_8_1",
      "first-page": "704",
      "volume-title": "Electron Microscopy 1990. Proceedings of the XIIth International Congress for Electron Microscopy",
      "author": "Ding B.",
      "year": "1990"
    },
    {
      "key": "e_1_2_1_9_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1007/BF00398090"
    },
    {
      "key": "e_1_2_1_10_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1104/pp.56.5.555"
    },
    {
      "key": "e_1_2_1_11_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/jemt.1060030206"
    },
    {
      "key": "e_1_2_1_12_1",
      "doi-asserted-by": "crossref",
      "first-page": "565",
      "DOI": "10.1111/1365-3040.ep11604101",
      "article-title": "The cells shed by the root cap of Zea : Their origin and some structural and physiological properties",
      "volume": "10",
      "author": "Guinel F. C.",
      "year": "1987",
      "journal-title": "Plant Cell Environ."
    },
    {
      "key": "e_1_2_1_13_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1365-2818.1984.tb04650.x"
    },
    {
      "key": "e_1_2_1_14_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0014-4827(72)90183-8"
    },
    {
      "key": "e_1_2_1_15_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1365-2818.1989.tb00591.x"
    },
    {
      "key": "e_1_2_1_16_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1007/BF01285037"
    },
    {
      "key": "e_1_2_1_17_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/jemt.1060040302"
    },
    {
      "key": "e_1_2_1_18_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1365-2818.1978.tb00116.x"
    },
    {
      "key": "e_1_2_1_19_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1007/978-3-642-72815-0_8"
    },
    {
      "key": "e_1_2_1_20_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1007/BF01279752"
    },
    {
      "key": "e_1_2_1_21_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0074-7696(08)61676-9"
    },
    {
      "key": "e_1_2_1_22_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.2307/2444616"
    },
    {
      "key": "e_1_2_1_23_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1365-2818.1982.tb00331.x"
    },
    {
      "key": "e_1_2_1_24_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0022-5320(69)90033-1"
    },
    {
      "key": "e_1_2_1_25_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1007/BF00395066"
    },
    {
      "key": "e_1_2_1_26_1",
      "first-page": "253",
      "article-title": "High Pressure freezing comes of age",
      "volume": "3",
      "author": "Studer D.",
      "year": "1989",
      "journal-title": "Scan Microsc."
    },
    {
      "key": "e_1_2_1_27_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1007/BF01276371"
    },
    {
      "key": "e_1_2_1_28_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1083/jcb.99.1.63s"
    },
    {
      "key": "e_1_2_1_29_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1104/pp.76.1.45"
    },
    {
      "key": "e_1_2_1_30_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1007/BF00395069"
    }
  ],
  "container-title": "Journal of Electron Microscopy Technique",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fjemt.1060190111",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "text-mining"
    },
    {
      "URL": "https://onlinelibrary.wiley.com/doi/pdf/10.1002/jemt.1060190111",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2023,
        10,
        22
      ]
    ],
    "date-time": "2023-10-22T21:48:05Z",
    "timestamp": 1698011285000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://onlinelibrary.wiley.com/doi/10.1002/jemt.1060190111"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1991,
        9
      ]
    ]
  },
  "references-count": 29,
  "journal-issue": {
    "issue": "1",
    "published-print": {
      "date-parts": [
        [
          1991,
          9
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1002/jemt.1060190111"
  ],
  "URL": "http://dx.doi.org/10.1002/jemt.1060190111",
  "relation": {},
  "ISSN": [
    "0741-0581",
    "1553-0817"
  ],
  "subject": [],
  "container-title-short": "J. Elec. Microsc. Tech.",
  "published": {
    "date-parts": [
      [
        1991,
        9
      ]
    ]
  }
}