DOI: 10.1002/jemt.1060180309. Negative staining‐carbon film technique: New cellular and molecular applications

Negative staining‐carbon film technique: New cellular and molecular applications

10.1002/jemt.1060180309

Crossref journal-article

Wiley

Journal of Electron Microscopy Technique (311)

Abstract

AbstractTwo techniques are presented which extend the original negative staining‐carbon film technique into new areas of cellular and molecular application. These relate (1) to the production of negatively stained specimens of single‐layer plasma membrane split from intact cells during the overall procedure that are negatively stained from the cytoplasmic face and (2) to the production of negatively stained specimens directly from glycerol‐containing protein solutions, membrane or viral suspensions. In both cases in vacuo drying onto mica from glycerol is performed, prior to deposition of a carbon film. (For the cellular technique, freshly cleaved mica is firstly rendered positively charged by immersion in Alcian blue.) This is followed by release of the carbon film plus adsorbed membrane or protein by floating onto water, with subsequent negative staining. Selected preliminary applications using human erythrocyte membrane and the high molecular weight (native) human erythrocyte tripeptidyl peptidase‐II complex are given and considered speculation as to the future application of the techniques is provided.

Bibliography

Robin Harris, J. (1991). Negative stainingâcarbon film technique: New cellular and molecular applications. Journal of Electron Microscopy Technique, 18(3), 269â276. Portico.

Authors 1

J. Robin Harris (first)

References 18 Referenced 11

10.1016/0014-4827(88)90333-3
10.1016/0167-4838(85)90182-7
{'key': 'e_1_2_1_4_1', 'first-page': '49', 'volume-title': 'Electron Microscopy of Proteins', 'author': 'Harris J.R.', 'year': '1982'} / Electron Microscopy of Proteins by Harris J.R. (1982)
{'key': 'e_1_2_1_5_1', 'first-page': '147', 'article-title': 'The production of paracrystalline two‐dimensional monolayers of purified protein molecules', 'volume': '13', 'author': 'Harris J.R.', 'year': '1982', 'journal-title': 'Micron'} / Micron / The production of paracrystalline two‐dimensional monolayers of purified protein molecules by Harris J.R. (1982)
10.1016/0739-6260(87)90014-1
{'key': 'e_1_2_1_7_1', 'first-page': '251', 'volume-title': 'Blood Cell Biochemistry, Vol. 1, Erythroid Cells', 'author': 'Harris J.R.', 'year': '1990'} / Blood Cell Biochemistry, Vol. 1, Erythroid Cells by Harris J.R. (1990)
10.1016/S0022-5320(70)90017-1
10.1016/0739-6260(89)90055-5
{'key': 'e_1_2_1_10_1', 'volume-title': 'Electron Microscopy of Tissues, Cells and Organelles', 'author': 'Harris J.R.', 'year': '1991'} / Electron Microscopy of Tissues, Cells and Organelles by Harris J.R. (1991)
10.1111/j.1365-2818.1976.tb01078.x
10.1016/0739-6260(90)90008-4
10.1099/0022-1317-31-2-265
10.1016/S0022-5320(75)80033-5
10.1016/S0022-5320(74)90015-X
10.1016/S0022-5320(75)80151-1
10.1242/jcs.49.1.383 / J. Cell Sci. / Ultrastructure of sheep erythrocyte plasma membranes and cytoskeletons bound to solid supports by Lang R.D. (1983)
{'key': 'e_1_2_1_18_1', 'first-page': '358', 'article-title': 'Separation of the lipid bilayer from the membrane skeleton during discocyte‐echinocyte transformation of human erythrocyte ghosts', 'volume': '49', 'author': 'Liu S.‐C.', 'year': '1989', 'journal-title': 'Eur. J. Cell Biol.'} / Eur. J. Cell Biol. / Separation of the lipid bilayer from the membrane skeleton during discocyte‐echinocyte transformation of human erythrocyte ghosts by Liu S.‐C. (1989)
10.1083/jcb.102.3.997

Dates

Type	When
Created	20 years, 5 months ago (Feb. 24, 2005, 3:28 a.m.)
Deposited	1 year, 9 months ago (Oct. 23, 2023, 3:08 a.m.)
Indexed	1 year, 9 months ago (Oct. 24, 2023, 1:24 a.m.)
Issued	34 years, 1 month ago (July 1, 1991)
Published	34 years, 1 month ago (July 1, 1991)
Published Online	20 years, 6 months ago (Feb. 4, 2005)
Published Print	34 years, 1 month ago (July 1, 1991)

Funders 0

None

BibTeX

@article{Robin_Harris_1991, title={Negative staining‐carbon film technique: New cellular and molecular applications}, volume={18}, ISSN={1553-0817}, url={http://dx.doi.org/10.1002/jemt.1060180309}, DOI={10.1002/jemt.1060180309}, number={3}, journal={Journal of Electron Microscopy Technique}, publisher={Wiley}, author={Robin Harris, J.}, year={1991}, month=jul, pages={269–276} }

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