DOI: 10.1002/jemt.1060180304. Tridimensional ultrastructure of perfusion fixed gastrointestinal epithelial cells by high resolution scanning electron microscopy

Tridimensional ultrastructure of perfusion fixed gastrointestinal epithelial cells by high resolution scanning electron microscopy

10.1002/jemt.1060180304

Crossref journal-article

Wiley

Journal of Electron Microscopy Technique (311)

Abstract

AbstractImprovements in the design of modern scanning electron microscopes (SEM) and new methods of specimen preparation incorporating chemical removal of the cytosol and cytoskeleton, now make it possible to view cells and their organelles in three dimensions (3D) at high magnification. In this experiment, high resolution SEM (HRSEM) utilizing new methods of tissue preparation was used to study the intracellular structures of the mouse ileum. In addition, in vivo intestinal perfusion was used to further enhance cellular preservation. Using these modifications it was possible to visualize, in 3D, the fine structure of intestinal epithelial cells and intracellular organelles such as the nucleus, mitochondria, endoplasmic reticulum, and Golgi complex, as well as microvilli and cell membrane. Whole mitochondria appeared as irregularly shaped organelles which contained tubular cristae. Plate‐like cristae were not observed. The brush border was found to be a closely packed array of cylindrical projections. The extensive folding and structural intricacy of lateral cell membranes between absorptive cells could only be appreciated by viewing this tissue with 3D HRSEM. The use of HRSEM to study 3D ultrastructure of cells and their organelles will improve our understanding of the structure‐function relationships in both the healthy and diseased gastrointestinal tract.

Bibliography

Kendall, C. W., Venketeshwer Rao, A., Janezic, S. A., Temkin, R. J., Hollenberg, M. J., & Lea, P. J. (1991). Tridimensional ultrastructure of perfusion fixed gastrointestinal epithelial cells by high resolution scanning electron microscopy. Journal of Electron Microscopy Technique, 18(3), 223â230. Portico.

Authors 6

Cyril W. Kendall (first)
A. Venketeshwer Rao (additional)
Susan A. Janezic (additional)
Robert J. Temkin (additional)
Martin J. Hollenberg (additional)
Peter J. Lea (additional)

References 26 Referenced 5

10.1016/S0014-4835(87)80009-X
10.1093/ajcn/34.2.218 / Am. J. Clin. Nutr. / Effect of chronic intake of dietary fiber on the ultrastructural topography of rat jejunum and colon: a scanning electron microscopy study by Cassidy M.M. (1981)
{'key': 'e_1_2_1_4_1', 'first-page': '661', 'article-title': 'Effect of molecular structure on bile acid‐induced alterations in absorptive function, permeability and morphology in the perfused rabbit colon', 'volume': '94', 'author': 'Chadwick V.S.', 'year': '1979', 'journal-title': 'J. Lab. Clin. Med.'} / J. Lab. Clin. Med. / Effect of molecular structure on bile acid‐induced alterations in absorptive function, permeability and morphology in the perfused rabbit colon by Chadwick V.S. (1979)
10.1002/path.1710980302
{'key': 'e_1_2_1_6_1', 'first-page': '1', 'volume-title': 'The Small Intestine', 'author': 'Creamer B.', 'year': '1974'} / The Small Intestine by Creamer B. (1974)
10.1177/22.6.401
10.1111/j.1365-2818.1975.tb04065.x
{'key': 'e_1_2_1_9_1', 'first-page': '1', 'volume-title': 'Practical Methods in Electron Microscopy', 'author': 'Glauert A.M.', 'year': '1974'} / Practical Methods in Electron Microscopy by Glauert A.M. (1974)
{'key': 'e_1_2_1_10_1', 'first-page': '19', 'volume-title': 'Small Intestine', 'author': 'Henry K.', 'year': '1982'} / Small Intestine by Henry K. (1982)
10.1016/0889-1605(85)90123-5
10.1002/sca.4950110402
{'key': 'e_1_2_1_13_1', 'first-page': '1221', 'article-title': 'An in vivo intestinal perfusion model for studying the effects of dietary components on intestinal ultrastructure: a method utilizing scanning electron microscopy', 'volume': '36', 'author': 'Kendall C.W.', 'year': '1987', 'journal-title': 'Nutr. Rep. Int.'} / Nutr. Rep. Int. / An in vivo intestinal perfusion model for studying the effects of dietary components on intestinal ultrastructure: a method utilizing scanning electron microscopy by Kendall C.W. (1987)
10.1017/S0424820100102158 / Proc. Electron Microsc. Soc. Am. / High resolution scanning electron microscopy of mitochondria from various rat tissues by Lea P.J. (1988)
10.1002/aja.1001840308
{'key': 'e_1_2_1_16_1', 'first-page': '63', 'volume-title': 'Cells and Tissues: A Three Dimensional Approach by Modern Techniques in Microscopy', 'author': 'Lea P.J.', 'year': '1989'} / Cells and Tissues: A Three Dimensional Approach by Modern Techniques in Microscopy by Lea P.J. (1989)
Lea P.J. Hollenberg M.J. Temkin R.J. andKhan P.A.(1990) Chemical extraction of the cytosol using osmium tetroxide for high resolution scanning electron microscopy.J. Electron Microsc. Tech.(in press).
{'key': 'e_1_2_1_18_1', 'first-page': '5', 'article-title': 'Human melanocytic nevi. I. Electron microscopy and three dimensional computer reconstruction of nevi and basement membrane zone from ultrathin serial sections', 'volume': '127', 'author': 'Lea P.J.', 'year': '1986', 'journal-title': 'Acta Dermatol. Venereol.'} / Acta Dermatol. Venereol. / Human melanocytic nevi. I. Electron microscopy and three dimensional computer reconstruction of nevi and basement membrane zone from ultrathin serial sections by Lea P.J. (1986)
10.1002/jemt.1060020305
{'key': 'e_1_2_1_20_1', 'first-page': '1111', 'article-title': 'Jejunal morphological consequences of vegetarian diets in humans', 'volume': '72', 'author': 'Owen R.L.', 'year': '1977', 'journal-title': 'Gastroenterology'} / Gastroenterology / Jejunal morphological consequences of vegetarian diets in humans by Owen R.L. (1977)
10.1093/jn/117.2.253
{'key': 'e_1_2_1_22_1', 'first-page': '1', 'volume-title': 'Gastrointestinal Ultrastructure: An Overview of Scanning and Transmission Electron Micrographs', 'author': 'Pfeiffer C.J.', 'year': '1974'} / Gastrointestinal Ultrastructure: An Overview of Scanning and Transmission Electron Micrographs by Pfeiffer C.J. (1974)
{'key': 'e_1_2_1_23_1', 'first-page': '179', 'article-title': "The gross light microscopic and scanning electron microscopic appearance of the early lesions of Crohn's disease", 'author': 'Rickert R.R.', 'year': '1977', 'journal-title': 'Scanning Electron Microsc.'} / Scanning Electron Microsc. / The gross light microscopic and scanning electron microscopic appearance of the early lesions of Crohn's disease by Rickert R.R. (1977)
10.1002/1097-0142(197711)40:5 <2509::AID-CNCR2820400918>3.0.CO;2-K
{'key': 'e_1_2_1_25_1', 'first-page': '1911', 'article-title': 'The application of electron microscopy in the clinical investigation of the human colon', 'author': 'Siew S.', 'year': '1983', 'journal-title': 'Scanning Electron Microsc.'} / Scanning Electron Microsc. / The application of electron microscopy in the clinical investigation of the human colon by Siew S. (1983)
10.1111/j.1365-2818.1984.tb00487.x
10.3109/01913128709048437

Dates

Type	When
Created	20 years, 6 months ago (Feb. 24, 2005, 3:28 a.m.)
Deposited	1 year, 10 months ago (Oct. 23, 2023, 3:08 a.m.)
Indexed	1 year, 2 months ago (June 6, 2024, 7:19 a.m.)
Issued	34 years, 1 month ago (July 1, 1991)
Published	34 years, 1 month ago (July 1, 1991)
Published Online	20 years, 6 months ago (Feb. 4, 2005)
Published Print	34 years, 1 month ago (July 1, 1991)

Funders 0

None

BibTeX

@article{Kendall_1991, title={Tridimensional ultrastructure of perfusion fixed gastrointestinal epithelial cells by high resolution scanning electron microscopy}, volume={18}, ISSN={1553-0817}, url={http://dx.doi.org/10.1002/jemt.1060180304}, DOI={10.1002/jemt.1060180304}, number={3}, journal={Journal of Electron Microscopy Technique}, publisher={Wiley}, author={Kendall, Cyril W. and Venketeshwer Rao, A. and Janezic, Susan A. and Temkin, Robert J. and Hollenberg, Martin J. and Lea, Peter J.}, year={1991}, month=jul, pages={223–230} }

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