DOI: 10.1002/jemt.1060170205. Scale formation in algae

10.1002/jemt.1060170205

Crossref journal-article

Wiley

Journal of Electron Microscopy Technique (311)

Abstract

AbstractScale biogenesis in algae represents a unique model system to study the transport of secretory macromolecules through the Golgi apparatus (GA) and their exocytosis. The larger scales can be visualized in the light microscope, and thus the kinetics of scale assembly, transport, and secretion can be studied in vivo. In addition, scales are osmiophilic and readily visible in conventional transmission electron microscopy; thus, details of scale assembly and sorting can be studied without invoking immunolabeling techniques. The following are distinctive features of scale biogenesis in algae: (1) transport of scales through the GA‐stack occurs by cisternal progression; (2) scale secretion may be very rapid (in some cases a single GA‐cisterna leaves the stack every 15–20 s); (3) sorting of different scale types does not occur in the GA, but in a post‐GA‐compartment. Recent progress in the analysis of scale formation in the green flagellates Tetraselmis and Scherffelia is reviewed.

Bibliography

Melkonian, M., Becker, B., & Becker, D. (1991). Scale formation in algae. Journal of Electron Microscopy Technique, 17(2), 165â178. Portico.

Authors 3

Michael Melkonian (first)
Burkhard Becker (additional)
Dieter Becker (additional)

References 64 Referenced 68

10.1016/S0254-6299(16)31617-9
10.1007/BF01418596
10.1111/j.1432-1033.1989.tb14811.x
10.1042/bj1420139
10.1083/jcb.41.1.109
{'key': 'e_1_2_1_7_1', 'first-page': '361', 'article-title': 'Movement of the protoplast and function of the Golgi apparatus in algae', 'volume': '7', 'author': 'Brown R. M.', 'year': '1971', 'journal-title': 'Publ. Wiss. Film Sektion Biologie'} / Publ. Wiss. Film Sektion Biologie / Movement of the protoplast and function of the Golgi apparatus in algae by Brown R. M. (1971)
{'key': 'e_1_2_1_8_1', 'first-page': '369', 'article-title': 'The Golgi apparatus and endomembrane system: its role in the biosynthesis, transport and secretion of wall constituents in Pleurochrysis', 'volume': '14', 'author': 'Brown R. M.', 'year': '1974', 'journal-title': 'Port. Acta Biol.'} / Port. Acta Biol. / The Golgi apparatus and endomembrane system: its role in the biosynthesis, transport and secretion of wall constituents in Pleurochrysis by Brown R. M. (1974)
10.1007/BF00386949
{'key': 'e_1_2_1_10_1', 'first-page': '537', 'article-title': 'Biogenesis and structure of Golgi‐derived cellulosic scales in Pleurochrysis. I. Role of the endomembrane system in scale assembly and exocytosis', 'volume': '28', 'author': 'Brown R. M.', 'year': '1976', 'journal-title': 'Applied Polymer Symposium'} / Applied Polymer Symposium / Biogenesis and structure of Golgi‐derived cellulosic scales in Pleurochrysis. I. Role of the endomembrane system in scale assembly and exocytosis by Brown R. M. (1976)
10.1083/jcb.45.2.246
10.1016/B978-0-12-455350-7.50017-1
10.1126/science.166.3907.894
10.1111/j.0022-3646.1984.00415.x
10.1093/jxb/38.8.1399
10.1242/jcs.52.1.351
10.1016/S0092-8674(85)80097-0
10.1146/annurev.cb.01.110185.002311
10.1083/jcb.91.3.77s
10.1093/jxb/22.4.959
10.1126/science.2945253
10.1083/jcb.108.2.277
10.1007/BF00385167
10.1007/BF01275788
{'key': 'e_1_2_1_25_1', 'first-page': '45', 'article-title': 'Antigenic properties of the Golgi derived scales of Pleurochrysis scherffelii', 'volume': '23', 'author': 'Kavookjian A. M. A.', 'year': '1978', 'journal-title': 'Cytobios'} / Cytobios / Antigenic properties of the Golgi derived scales of Pleurochrysis scherffelii by Kavookjian A. M. A. (1978)
10.1099/00221287-19-1-87
10.1017/S0025315400054266
10.1017/S0025315400016568
10.1017/S0025315400005385
10.1093/jxb/24.1.223
10.1007/BF01276600
10.1111/j.1756-1051.1986.tb00875.x
10.1007/BF01285039
10.1007/BF01281970
{'key': 'e_1_2_1_35_1', 'first-page': '73', 'volume-title': 'The Systematics of Green Algae', 'author': 'Melkonian M.', 'year': '1984'} / The Systematics of Green Algae by Melkonian M. (1984)
{'key': 'e_1_2_1_36_1', 'first-page': '297', 'volume-title': 'Cell‐Free Analysis of Membrane Traffic', 'author': 'Melkonian M.', 'year': '1988'} / Cell‐Free Analysis of Membrane Traffic by Melkonian M. (1988)
10.1111/j.1438-8677.1986.tb02967.x
10.1111/j.1756-1051.1986.tb00876.x
10.2216/i0031-8884-20-4-365.1
{'key': 'e_1_2_1_40_1', 'first-page': '261', 'volume-title': 'Cell Walls 81', 'author': 'Melkonian M.', 'year': '1981'} / Cell Walls 81 by Melkonian M. (1981)
{'key': 'e_1_2_1_41_1', 'first-page': '207', 'article-title': 'Isolation and characterization of flagellar scales from the scaly green flagellate Tetraselmis. / B', 'volume': '18', 'author': 'Melkonian M.', 'year': '1983', 'journal-title': 'Phycol. J.'} / Phycol. J. / Isolation and characterization of flagellar scales from the scaly green flagellate Tetraselmis. / B by Melkonian M. (1983)
10.2216/i0031-8884-21-4-427.1
10.1083/jcb.49.1.212
{'key': 'e_1_2_1_44_1', 'first-page': '25', 'article-title': 'Kinetics of Golgi apparatus membrane flux following monensin treatment of embryogenic carrot cells', 'volume': '30', 'author': 'Morré D. J.', 'year': '1983', 'journal-title': 'Eur. J. Cell Biol.'} / Eur. J. Cell Biol. / Kinetics of Golgi apparatus membrane flux following monensin treatment of embryogenic carrot cells by Morré D. J. (1983)
10.1007/BF00384849
10.1016/0304-4157(79)90008-X
{'key': 'e_1_2_1_47_1', 'first-page': '84', 'volume-title': 'Dynamic Aspects of Plant Ultrastructure', 'author': 'Morré D. J.', 'year': '1974'} / Dynamic Aspects of Plant Ultrastructure by Morré D. J. (1974)
10.1007/978-3-540-36396-5_4
{'key': 'e_1_2_1_49_1', 'first-page': '85', 'volume-title': 'Phytoflagellates. Developments in Marine Biol.', 'author': 'Norris R. E.', 'year': '1980'} / Phytoflagellates. Developments in Marine Biol. by Norris R. E. (1980)
10.1126/science.1096303
10.1017/S0025315400054989
10.1007/BF02364697
10.1093/jxb/28.2.416
10.1080/00071617900650241
10.1016/S0074-7696(08)62465-1
10.1007/978-3-7091-8602-2_2
{'key': 'e_1_2_1_57_1', 'first-page': '587', 'article-title': 'Biogenesis and structure of Golgi‐derived cellulosic scales in Pleurochrysis. II. Scale composition and supramolecular structure', 'volume': '28', 'author': 'Romanovicz D. K.', 'year': '1976', 'journal-title': 'Applied Polymer Symposium'} / Applied Polymer Symposium / Biogenesis and structure of Golgi‐derived cellulosic scales in Pleurochrysis. II. Scale composition and supramolecular structure by Romanovicz D. K. (1976)
{'key': 'e_1_2_1_58_1', 'first-page': '542', 'article-title': 'Secretory activities of the root cap', 'volume': '13', 'author': 'Rougier M.', 'year': '1981', 'journal-title': 'Encyclopedia of Plant Physiology New Series'} / Encyclopedia of Plant Physiology New Series / Secretory activities of the root cap by Rougier M. (1981)
10.1007/BF01284093
10.1111/j.1399-3054.1988.tb06646.x
10.1016/0968-0004(82)90131-1
10.1016/S0065-2318(08)60313-3
10.1016/S0074-7696(08)61476-X
10.1083/jcb.54.3.507
10.1016/0008-6215(85)85228-9

Dates

Type	When
Created	20 years, 5 months ago (Feb. 24, 2005, 3:37 a.m.)
Deposited	1 year, 9 months ago (Oct. 22, 2023, 10:37 p.m.)
Indexed	1 year, 2 months ago (June 13, 2024, 3:48 a.m.)
Issued	34 years, 6 months ago (Feb. 1, 1991)
Published	34 years, 6 months ago (Feb. 1, 1991)
Published Online	20 years, 6 months ago (Feb. 4, 2005)
Published Print	34 years, 6 months ago (Feb. 1, 1991)

Funders 0

None

BibTeX

@article{Melkonian_1991, title={Scale formation in algae}, volume={17}, ISSN={1553-0817}, url={http://dx.doi.org/10.1002/jemt.1060170205}, DOI={10.1002/jemt.1060170205}, number={2}, journal={Journal of Electron Microscopy Technique}, publisher={Wiley}, author={Melkonian, Michael and Becker, Burkhard and Becker, Dieter}, year={1991}, month=feb, pages={165–178} }

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