DOI: 10.1002/jemt.1060160407. Evaporation during preparation of unsupported thin vitrified aqueous layers for cryo‐electron microscopy

Evaporation during preparation of unsupported thin vitrified aqueous layers for cryo‐electron microscopy

10.1002/jemt.1060160407

Crossref journal-article

Wiley

Journal of Electron Microscopy Technique (311)

Abstract

AbstractEvaporation of water cannot be fully avoided when an unsupported thin vitrified film of an aqueous suspension is prepared for cryo‐electron microscopy. This results in increasing concentration of solute which could affect the observed material. We have quantitatively studied this effect by measuring the contrast of polystyrene spheres in a metrizamide solution. The drying effect is generally negligible when specimens are prepared on a hydrophilic perforated support but it is frequently important when hydrophobic films are used instead. A flow of humid air, double blotting with minimal exposure of the thin liquid film to the atmosphere, or an automatic plunger optimizing the blotting conditions are simple methods for reducing drying effects. With this third device acting on a hydrophilic supporting film, the increase of solute concentration is limited to less than 20%.

Bibliography

Cyrklaff, M., Adrian, M., & Dubochet, J. (1990). Evaporation during preparation of unsupported thin vitrified aqueous layers for cryoâelectron microscopy. Journal of Electron Microscopy Technique, 16(4), 351â355. Portico.

Authors 3

Marek Cyrklaff (first)
Marc Adrian (additional)
Jacques Dubochet (additional)

References 11 Referenced 47

10.1038/308032a0
10.1002/jemt.1060100111
10.1017/S0033583500004297
10.1002/j.1460-2075.1986.tb04241.x
10.1016/0304-3991(82)90187-5
10.1111/j.1365-2818.1982.tb04625.x
10.1111/j.1365-2818.1982.tb04626.x
10.1016/S0022-5320(71)80152-1
10.1016/0304-3991(84)90051-2
{'key': 'e_1_2_1_11_1', 'volume-title': 'Centrifugation in Biology and Medical Science', 'author': 'Sheeler P.', 'year': '1981'} / Centrifugation in Biology and Medical Science by Sheeler P. (1981)
10.1016/0304-3991(89)90076-4

Dates

Type	When
Created	20 years, 6 months ago (Feb. 24, 2005, 3:43 a.m.)
Deposited	1 year, 10 months ago (Oct. 22, 2023, 3:37 p.m.)
Indexed	3 months, 2 weeks ago (May 12, 2025, 10:25 a.m.)
Issued	34 years, 8 months ago (Dec. 1, 1990)
Published	34 years, 8 months ago (Dec. 1, 1990)
Published Online	20 years, 6 months ago (Feb. 4, 2005)
Published Print	34 years, 8 months ago (Dec. 1, 1990)

Funders 0

None

BibTeX

@article{Cyrklaff_1990, title={Evaporation during preparation of unsupported thin vitrified aqueous layers for cryo‐electron microscopy}, volume={16}, ISSN={1553-0817}, url={http://dx.doi.org/10.1002/jemt.1060160407}, DOI={10.1002/jemt.1060160407}, number={4}, journal={Journal of Electron Microscopy Technique}, publisher={Wiley}, author={Cyrklaff, Marek and Adrian, Marc and Dubochet, Jacques}, year={1990}, month=dec, pages={351–355} }

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