DOI: 10.1002/jemt.1060110103. Image analysis of electron micrographs relating to mineralization in calcifying cartilage: Theoretical considerations

Image analysis of electron micrographs relating to mineralization in calcifying cartilage: Theoretical considerations

10.1002/jemt.1060110103

Crossref journal-article

Wiley

Journal of Electron Microscopy Technique (311)

Abstract

AbstractBiological mineralization is a cell‐mediated process which is believed to be triggered by a “nucleating agent.” Various matrix structures, such as matrix vesicles, collagen fibrils and macromolecules, have been claimed to be the source of this substance, since these components have been found by transmission electron microscopy (TEM) of thin sections to be associated with early mineral crystals. Systematic image analysis of the relationships revealed in electron micrographs between specific matrix components and early mineral deposits has shown that unequivocal image interpretation is not possible. This is due principally to the problems posed by overprojection and truncation phenomena, since the structures being analyzed lie within the same dimensional range as thin section thickness. Various examples are illustrated and discussed. The site at which mineral crystals are initially laid down thus cannot be identified with any matrix structure using thin section TEM. Possible technical approaches to resolve this problem of image analysis are discussed.

Bibliography

Hunziker, E. B., Herrmann, W., CruzâOrive, L. M., & Arsenault, A. L. (1989). Image analysis of electron micrographs relating to mineralization in calcifying cartilage: Theoretical considerations. Journal of Electron Microscopy Technique, 11(1), 9â15. Portico.

Authors 4

E. B. Hunziker (first)
W. Herrmann (additional)
L. M. Cruz‐Orive (additional)
A. L. Arsenault (additional)

References 26 Referenced 10

{'key': 'e_1_2_1_2_1', 'first-page': '135', 'article-title': 'Analysis of matrix vesicles and their role in the calcification of epiphyseal cartilage', 'volume': '35', 'author': 'Ali S. Y.', 'year': '1976', 'journal-title': 'Fed. Proc.'} / Fed. Proc. / Analysis of matrix vesicles and their role in the calcification of epiphyseal cartilage by Ali S. Y. (1976)
10.1083/jcb.41.1.59
{'key': 'e_1_2_1_4_1', 'first-page': '105', 'article-title': 'Matrix vesicle calcification', 'volume': '35', 'author': 'Anderson H. C.', 'year': '1976', 'journal-title': 'Fed. Proc.'} / Fed. Proc. / Matrix vesicle calcification by Anderson H. C. (1976)
{'key': 'e_1_2_1_5_1', 'first-page': '364', 'volume-title': 'The Chemistry and Biology of Mineralized Tissues', 'author': 'Arsenault A. L.', 'year': '1985'} / The Chemistry and Biology of Mineralized Tissues by Arsenault A. L. (1985)
10.1007/BF02555138 / Crystals in calcified epiphyseal cartilage and cortical bone of the rat by Arsenault A. L. (1988)
10.1007/BF02556344
10.1016/S0022-5320(67)80034-0
10.1007/BF00337312
10.1097/00003086-198106000-00036 / Clin. Orthop. Rel. Res. / Current concepts of the physiology and biochemistry of calcification by Boskey A. L. (1981)
10.1098/rspa.1970.0119
10.1111/j.1365-2818.1983.tb04255.x
10.1111/j.1365-2818.1986.tb02765.x
{'key': 'e_1_2_1_14_1', 'first-page': '35', 'article-title': 'The role of proteoglycans at the beginning of the calcification process: Histochemical and ultrastructural observations', 'volume': '26', 'author': "Dell'Orbo C.", 'year': '1982', 'journal-title': 'Bas. Appl. Histochem.'} / Bas. Appl. Histochem. / The role of proteoglycans at the beginning of the calcification process: Histochemical and ultrastructural observations by Dell'Orbo C. (1982)
10.1038/217130a0
10.1098/rspb.1957.0010
{'key': 'e_1_2_1_17_1', 'first-page': '25', 'volume-title': 'Handbook of Physiology—Endocrinology (VII)', 'author': 'Glimcher M. J.', 'year': '1974'} / Handbook of Physiology—Endocrinology (VII) by Glimcher M. J. (1974)
{'key': 'e_1_2_1_18_1', 'volume-title': 'Petrographic Methods and Calculations', 'author': 'Holmes A.', 'year': '1927'} / Petrographic Methods and Calculations by Holmes A. (1927)
10.1083/jcb.98.1.267
10.2307/2528765
10.1016/S0022-5320(82)80001-4
10.1016/0221-8747(83)90014-0
10.1097/00003086-198607000-00024 / Clin. Orthop. Rel. Res. / Chondrocalcin and the calcification of cartilage by Poole A. R. (1986)
10.1016/S0022-5320(82)80040-3
{'key': 'e_1_2_1_25_1', 'first-page': '609', 'volume-title': 'Proceedings of the 4th European Congress on Electron Microscopy', 'author': 'Small J. V.', 'year': '1968'} / Proceedings of the 4th European Congress on Electron Microscopy by Small J. V. (1968)
10.1016/S0022-5320(74)80056-0
10.1016/S0022-5320(70)90181-4

Dates

Type	When
Created	20 years, 6 months ago (Feb. 24, 2005, 4:18 a.m.)
Deposited	1 year, 10 months ago (Oct. 22, 2023, 12:03 a.m.)
Indexed	1 year, 10 months ago (Oct. 24, 2023, 12:59 a.m.)
Issued	36 years, 8 months ago (Jan. 1, 1989)
Published	36 years, 8 months ago (Jan. 1, 1989)
Published Online	20 years, 6 months ago (Feb. 4, 2005)
Published Print	36 years, 8 months ago (Jan. 1, 1989)

Funders 0

None

BibTeX

@article{Hunziker_1989, title={Image analysis of electron micrographs relating to mineralization in calcifying cartilage: Theoretical considerations}, volume={11}, ISSN={1553-0817}, url={http://dx.doi.org/10.1002/jemt.1060110103}, DOI={10.1002/jemt.1060110103}, number={1}, journal={Journal of Electron Microscopy Technique}, publisher={Wiley}, author={Hunziker, E. B. and Herrmann, W. and Cruz‐Orive, L. M. and Arsenault, A. L.}, year={1989}, month=jan, pages={9–15} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2023,
        10,
        24
      ]
    ],
    "date-time": "2023-10-24T04:59:42Z",
    "timestamp": 1698123582074
  },
  "reference-count": 26,
  "publisher": "Wiley",
  "issue": "1",
  "license": [
    {
      "start": {
        "date-parts": [
          [
            2005,
            2,
            4
          ]
        ],
        "date-time": "2005-02-04T00:00:00Z",
        "timestamp": 1107475200000
      },
      "content-version": "vor",
      "delay-in-days": 5878,
      "URL": "http://onlinelibrary.wiley.com/termsAndConditions#vor"
    }
  ],
  "content-domain": {
    "domain": [],
    "crossmark-restriction": false
  },
  "published-print": {
    "date-parts": [
      [
        1989,
        1
      ]
    ]
  },
  "abstract": "<jats:title>Abstract</jats:title><jats:p>Biological mineralization is a cell\u2010mediated process which is believed to be triggered by a \u201cnucleating agent.\u201d Various matrix structures, such as matrix vesicles, collagen fibrils and macromolecules, have been claimed to be the source of this substance, since these components have been found by transmission electron microscopy (TEM) of thin sections to be associated with early mineral crystals. Systematic image analysis of the relationships revealed in electron micrographs between specific matrix components and early mineral deposits has shown that unequivocal image interpretation is not possible. This is due principally to the problems posed by overprojection and truncation phenomena, since the structures being analyzed lie within the same dimensional range as thin section thickness. Various examples are illustrated and discussed. The site at which mineral crystals are initially laid down thus cannot be identified with any matrix structure using thin section TEM. Possible technical approaches to resolve this problem of image analysis are discussed.</jats:p>",
  "DOI": "10.1002/jemt.1060110103",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2005,
        2,
        24
      ]
    ],
    "date-time": "2005-02-24T09:18:12Z",
    "timestamp": 1109236692000
  },
  "page": "9-15",
  "source": "Crossref",
  "is-referenced-by-count": 10,
  "title": "Image analysis of electron micrographs relating to mineralization in calcifying cartilage: Theoretical considerations",
  "prefix": "10.1002",
  "volume": "11",
  "author": [
    {
      "given": "E. B.",
      "family": "Hunziker",
      "sequence": "first",
      "affiliation": []
    },
    {
      "given": "W.",
      "family": "Herrmann",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "L. M.",
      "family": "Cruz\u2010Orive",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "A. L.",
      "family": "Arsenault",
      "sequence": "additional",
      "affiliation": []
    }
  ],
  "member": "311",
  "published-online": {
    "date-parts": [
      [
        2005,
        2,
        4
      ]
    ]
  },
  "reference": [
    {
      "key": "e_1_2_1_2_1",
      "first-page": "135",
      "article-title": "Analysis of matrix vesicles and their role in the calcification of epiphyseal cartilage",
      "volume": "35",
      "author": "Ali S. Y.",
      "year": "1976",
      "journal-title": "Fed. Proc."
    },
    {
      "key": "e_1_2_1_3_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1083/jcb.41.1.59"
    },
    {
      "key": "e_1_2_1_4_1",
      "first-page": "105",
      "article-title": "Matrix vesicle calcification",
      "volume": "35",
      "author": "Anderson H. C.",
      "year": "1976",
      "journal-title": "Fed. Proc."
    },
    {
      "key": "e_1_2_1_5_1",
      "first-page": "364",
      "volume-title": "The Chemistry and Biology of Mineralized Tissues",
      "author": "Arsenault A. L.",
      "year": "1985"
    },
    {
      "key": "e_1_2_1_6_1",
      "doi-asserted-by": "crossref",
      "DOI": "10.1007/BF02555138",
      "volume-title": "Crystals in calcified epiphyseal cartilage and cortical bone of the rat",
      "author": "Arsenault A. L.",
      "year": "1988"
    },
    {
      "key": "e_1_2_1_7_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1007/BF02556344"
    },
    {
      "key": "e_1_2_1_8_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0022-5320(67)80034-0"
    },
    {
      "key": "e_1_2_1_9_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1007/BF00337312"
    },
    {
      "key": "e_1_2_1_10_1",
      "doi-asserted-by": "crossref",
      "first-page": "225",
      "DOI": "10.1097/00003086-198106000-00036",
      "article-title": "Current concepts of the physiology and biochemistry of calcification",
      "volume": "157",
      "author": "Boskey A. L.",
      "year": "1981",
      "journal-title": "Clin. Orthop. Rel. Res."
    },
    {
      "key": "e_1_2_1_11_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1098/rspa.1970.0119"
    },
    {
      "key": "e_1_2_1_12_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1365-2818.1983.tb04255.x"
    },
    {
      "key": "e_1_2_1_13_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1365-2818.1986.tb02765.x"
    },
    {
      "key": "e_1_2_1_14_1",
      "first-page": "35",
      "article-title": "The role of proteoglycans at the beginning of the calcification process: Histochemical and ultrastructural observations",
      "volume": "26",
      "author": "Dell'Orbo C.",
      "year": "1982",
      "journal-title": "Bas. Appl. Histochem."
    },
    {
      "key": "e_1_2_1_15_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/217130a0"
    },
    {
      "key": "e_1_2_1_16_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1098/rspb.1957.0010"
    },
    {
      "key": "e_1_2_1_17_1",
      "first-page": "25",
      "volume-title": "Handbook of Physiology\u2014Endocrinology (VII)",
      "author": "Glimcher M. J.",
      "year": "1974"
    },
    {
      "key": "e_1_2_1_18_1",
      "volume-title": "Petrographic Methods and Calculations",
      "author": "Holmes A.",
      "year": "1927"
    },
    {
      "key": "e_1_2_1_19_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1083/jcb.98.1.267"
    },
    {
      "key": "e_1_2_1_20_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.2307/2528765"
    },
    {
      "key": "e_1_2_1_21_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0022-5320(82)80001-4"
    },
    {
      "key": "e_1_2_1_22_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0221-8747(83)90014-0"
    },
    {
      "key": "e_1_2_1_23_1",
      "doi-asserted-by": "crossref",
      "first-page": "114",
      "DOI": "10.1097/00003086-198607000-00024",
      "article-title": "Chondrocalcin and the calcification of cartilage",
      "volume": "208",
      "author": "Poole A. R.",
      "year": "1986",
      "journal-title": "Clin. Orthop. Rel. Res."
    },
    {
      "key": "e_1_2_1_24_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0022-5320(82)80040-3"
    },
    {
      "key": "e_1_2_1_25_1",
      "first-page": "609",
      "volume-title": "Proceedings of the 4th European Congress on Electron Microscopy",
      "author": "Small J. V.",
      "year": "1968"
    },
    {
      "key": "e_1_2_1_26_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0022-5320(74)80056-0"
    },
    {
      "key": "e_1_2_1_27_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0022-5320(70)90181-4"
    }
  ],
  "container-title": "Journal of Electron Microscopy Technique",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fjemt.1060110103",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "text-mining"
    },
    {
      "URL": "https://onlinelibrary.wiley.com/doi/pdf/10.1002/jemt.1060110103",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2023,
        10,
        22
      ]
    ],
    "date-time": "2023-10-22T04:03:37Z",
    "timestamp": 1697947417000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://onlinelibrary.wiley.com/doi/10.1002/jemt.1060110103"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1989,
        1
      ]
    ]
  },
  "references-count": 26,
  "journal-issue": {
    "issue": "1",
    "published-print": {
      "date-parts": [
        [
          1989,
          1
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1002/jemt.1060110103"
  ],
  "URL": "http://dx.doi.org/10.1002/jemt.1060110103",
  "relation": {},
  "ISSN": [
    "0741-0581",
    "1553-0817"
  ],
  "subject": [],
  "container-title-short": "J. Elec. Microsc. Tech.",
  "published": {
    "date-parts": [
      [
        1989,
        1
      ]
    ]
  }
}