Abstract
AbstractDetails are given concerning the construction and use of a simple device for preparing samples of frozen‐hydrated biological material embedded in thin films of vitreous ice. The control of humidity around the sample and rate of freezing are of prime importance to obtain good specimens for cryo‐electron microscopy. Symptoms of ill performance of such an apparatus and poor cryo‐manipulation are discussed.
References
8
Referenced
19
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Murray J. M. andWard R. (1986):Preparation of holey carbon grids suitable for cryo‐electron microscopy. J. Elec. Mic. Tech. (this edition).
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Ward R. andMurray J. M. (1986):Natural propane cryogen for frozen‐hydrated biological specimens. J. Elec. Mic. Tech. (this edition).
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Dates
Type | When |
---|---|
Created | 20 years, 5 months ago (Feb. 24, 2005, 2:32 a.m.) |
Deposited | 1 year, 10 months ago (Oct. 21, 2023, 12:14 p.m.) |
Indexed | 1 year, 9 months ago (Oct. 29, 2023, 4:19 a.m.) |
Issued | 38 years, 5 months ago (March 1, 1987) |
Published | 38 years, 5 months ago (March 1, 1987) |
Published Online | 20 years, 6 months ago (Feb. 4, 2005) |
Published Print | 38 years, 5 months ago (March 1, 1987) |
@article{Murray_1987, title={Principles for the construction and operation of a device for rapidly freezing suspensions for cryo‐electron microscopy}, volume={5}, ISSN={1553-0817}, url={http://dx.doi.org/10.1002/jemt.1060050308}, DOI={10.1002/jemt.1060050308}, number={3}, journal={Journal of Electron Microscopy Technique}, publisher={Wiley}, author={Murray, John M. and Ward, Rob}, year={1987}, month=mar, pages={279–284} }