Abstract
AbstractThe properties of natural propane, mixed with 0–4% isopentane, as a cryogen suitable for rapid freezing of this layers of aqueous biological specimen suspensions are discussed. Although natural propane has rather variable properties, its freezing point can be depressed below the temperature of liquid nitrogen by adding a smaller amount of isopentane than is required for depressing the freezing point of pure propane.
References
7
Referenced
7
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Murray J. M. andWard R. (1986a)Structure of eukaryotic flagellar components by cryo‐electron microscopy. Electron Microsc. Soc. Am. Proc. 1986 pp98–101.
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Murray J. M. andWard R. (1986b)Preparation of holey carbon grids suitable for cryo‐electron microscopy. J. Electron Microsc. Tech. (this edition).
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Murray J. M.andWard R.(1986c)Principles for the construction and operation of a device for rapidly freezing suspensions for cryoelectron microscopy. J. Electron Microsc. Tech. (this edition).
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Dates
Type | When |
---|---|
Created | 20 years, 6 months ago (Feb. 24, 2005, 2:32 a.m.) |
Deposited | 1 year, 10 months ago (Oct. 21, 2023, 12:14 p.m.) |
Indexed | 1 year, 10 months ago (Oct. 22, 2023, 9:41 a.m.) |
Issued | 38 years, 5 months ago (March 1, 1987) |
Published | 38 years, 5 months ago (March 1, 1987) |
Published Online | 20 years, 6 months ago (Feb. 4, 2005) |
Published Print | 38 years, 5 months ago (March 1, 1987) |
@article{Ward_1987, title={Natural propane cryogen for frozen‐hydrated biological specimens}, volume={5}, ISSN={1553-0817}, url={http://dx.doi.org/10.1002/jemt.1060050307}, DOI={10.1002/jemt.1060050307}, number={3}, journal={Journal of Electron Microscopy Technique}, publisher={Wiley}, author={Ward, Rob and Murray, John M.}, year={1987}, month=mar, pages={275–277} }