DOI: 10.1002/jemt.1060050305. Transmission and scanning electron microscopic examination of intracellular organelles in freeze‐substituted <i>Kloeckera</i> and <i>Saccharomyces</i> cerevisiae Yeast Cells

Transmission and scanning electron microscopic examination of intracellular organelles in freeze‐substituted Kloeckera and Saccharomyces cerevisiae Yeast Cells

10.1002/jemt.1060050305

Crossref journal-article

Wiley

Journal of Electron Microscopy Technique (311)

Abstract

AbstractThe application of the conventional double‐fixation method (glutaraldehyde and osmium tetroxide) to whole yeast cells is difficult because the thick cell wall of the yeast prevents the penetration of osmium tetroxide. However, this problem was solved by using the freeze‐substitution fixation method. Therefore, it was possible to examine the intracellular structures of the yeast cells without digestion of the cell wall. In the present method, specimens for transmission electron microscopy and for scanning electron microscopy were prepared simultaneously. By scanning electron microscopic observation, three‐dimensional information about internal structures was obtained. In the cytological analysis of the yeasts, intracellular structures were well preserved by using the freeze‐substitution fixation method. On the outer leaflet of the nuclear envelope, many ribosomes were attached. The rough endoplasmic reticulum and Golgi apparatus were clearly seen in the yeast cytoplasm. The Golgi stack appeared to consist of smooth membranes, and small vesicles were present beside it. The details of other structures such as the nuclear division apparatus, actinlike filaments, and viruslike particles were also revealed. The present technique can be applied to most species of yeast cells. With this new information, the previous model of a yeast cell was modified.

Bibliography

Baba, M., & Osumi, M. (1987). Transmission and scanning electron microscopic examination of intracellular organelles in freezeâsubstituted Kloeckera and Saccharomyces cerevisiae Yeast Cells. Journal of Electron Microscopy Technique, 5(3), 249â261. Portico.

Authors 2

Misuzu Baba (first)
Masako Osumi (additional)

References 48 Referenced 78

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{'key': 'e_1_2_1_45_1', 'first-page': '199', 'article-title': 'Plasma membrane ultrastructural differences between exponential and stationary phases of Saccharomyces cerevisiae as revealed by freeze‐etching', 'volume': '81', 'author': 'Takeo K.', 'year': '1976', 'journal-title': 'J. Gen. Micro.'} / J. Gen. Micro. / Plasma membrane ultrastructural differences between exponential and stationary phases of Saccharomyces cerevisiae as revealed by freeze‐etching by Takeo K. (1976)
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Dates

Type	When
Created	20 years, 6 months ago (Feb. 24, 2005, 2:32 a.m.)
Deposited	1 year, 10 months ago (Oct. 21, 2023, 12:14 p.m.)
Indexed	1 month, 3 weeks ago (July 2, 2025, 2:32 p.m.)
Issued	38 years, 5 months ago (March 1, 1987)
Published	38 years, 5 months ago (March 1, 1987)
Published Online	20 years, 6 months ago (Feb. 4, 2005)
Published Print	38 years, 5 months ago (March 1, 1987)

Funders 0

None

BibTeX

@article{Baba_1987, title={Transmission and scanning electron microscopic examination of intracellular organelles in freeze‐substituted Kloeckera and Saccharomyces cerevisiae Yeast Cells}, volume={5}, ISSN={1553-0817}, url={http://dx.doi.org/10.1002/jemt.1060050305}, DOI={10.1002/jemt.1060050305}, number={3}, journal={Journal of Electron Microscopy Technique}, publisher={Wiley}, author={Baba, Misuzu and Osumi, Masako}, year={1987}, month=mar, pages={249–261} }

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