DOI: 10.1002/jemt.10421. Fluorescence lifetime imaging by time‐correlated single‐photon counting

Fluorescence lifetime imaging by time‐correlated single‐photon counting

10.1002/jemt.10421

Crossref journal-article

Wiley

Microscopy Research and Technique (311)

Abstract

AbstractWe present a time‐correlated single photon counting (TCPSC) technique that allows time‐resolved multi‐wavelength imaging in conjunction with a laser scanning microscope and a pulsed excitation source. The technique is based on a four‐dimensional histogramming process that records the photon density over the time of the fluorescence decay, the x‐y coordinates of the scanning area, and the wavelength. The histogramming process avoids any time gating or wavelength scanning and, therefore, yields a near‐perfect counting efficiency. The time resolution is limited only by the transit time spread of the detector. The technique can be used with almost any confocal or two‐photon laser scanning microscope and works at any scanning rate. We demonstrate the application to samples stained with several dyes and to CFP‐YFP FRET. Microsc. Res. Tech. 63:58–66, 2004. © 2003 Wiley‐Liss, Inc.

Bibliography

Becker, W., Bergmann, A., Hink, M. A., KÃ¶nig, K., Benndorf, K., & Biskup, C. (2003). Fluorescence lifetime imaging by timeâcorrelated singleâphoton counting. Microscopy Research and Technique, 63(1), 58â66. Portico.

Authors 6

W. Becker (first)
A. Bergmann (additional)
M.A. Hink (additional)
K. König (additional)
K. Benndorf (additional)
C. Biskup (additional)

References 60 Referenced 411

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Dates

Type	When
Created	21 years, 8 months ago (Dec. 11, 2003, 4:01 a.m.)
Deposited	1 year, 11 months ago (Sept. 11, 2023, 11:06 a.m.)
Indexed	4 days, 2 hours ago (Aug. 30, 2025, 12:27 p.m.)
Issued	21 years, 8 months ago (Dec. 10, 2003)
Published	21 years, 8 months ago (Dec. 10, 2003)
Published Online	21 years, 8 months ago (Dec. 10, 2003)
Published Print	21 years, 8 months ago (Jan. 1, 2004)

Funders 0

None

BibTeX

@article{Becker_2003, title={Fluorescence lifetime imaging by time‐correlated single‐photon counting}, volume={63}, ISSN={1097-0029}, url={http://dx.doi.org/10.1002/jemt.10421}, DOI={10.1002/jemt.10421}, number={1}, journal={Microscopy Research and Technique}, publisher={Wiley}, author={Becker, W. and Bergmann, A. and Hink, M.A. and König, K. and Benndorf, K. and Biskup, C.}, year={2003}, month=dec, pages={58–66} }

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  "container-title-short": "Microscopy Res &amp; Technique",
  "published": {
    "date-parts": [
      [
        2003,
        12,
        10
      ]
    ]
  }
}