DOI: 10.1002/jcp.1041590107. Coordinate and independent regulation of αB‐crystallin and HSP27 expression in response to physiological stress

Coordinate and independent regulation of αB‐crystallin and HSP27 expression in response to physiological stress

10.1002/jcp.1041590107

Crossref journal-article

Wiley

Journal of Cellular Physiology (311)

Abstract

Abstractα‐Crystallins share structural and functional properties with the stress protein hsp27. These polypeptides are expressed at low constitutive levels in many tissues including brain, and αB‐crystallin and hsp27 can accumulate in central nervous system glia in a variety of neurological conditions. We report here that heat shock and exposure to transition metals result in an increase in the steady state mRNA level of αB‐crystallin and hsp27 in primary cultures of rat forebrain astrocytes. Both exposure to tumour necrosis factor‐α and hypertonic conditions result in αB‐crystallin mRNA accumulation but no change in the hsp27 mRNA level. Under some of these conditions increased synthesis and accumulation of αB‐crystallin and hsp27 protein are also evident. We are unable to detect αA‐crystallin mRNA in resting or stressed astrocytes. A novel phenomenon involving a transitory change in stress protein mRNA mobility in Northern blots during induction is reported, which is stress type and cell type independent. The results demonstrate multiple stress regulation of αB‐crystallin and hsp27 in cultured astrocytes, suggesting that they can legitimately be regarded as stress proteins in the central nervous system. © 1994 wiley‐Liss, Inc.

Bibliography

Head, M. W., Corbin, E., & Goldman, J. E. (1994). Coordinate and independent regulation of Î±Bâcrystallin and HSP27 expression in response to physiological stress. Journal of Cellular Physiology, 159(1), 41â50. Portico.

Authors 3

Mark W. Head (first)
Elaine Corbin (additional)
James E. Goldman (additional)

References 63 Referenced 95

10.1093/nar/18.20.6049
{'key': 'e_1_2_1_3_1', 'first-page': '73', 'volume-title': 'Nucleic Acid Hybridisation', 'author': 'Anderson M. L. M.', 'year': '1985'} / Nucleic Acid Hybridisation by Anderson M. L. M. (1985)
10.1128/MCB.13.3.1824
10.1128/MCB.8.12.5059
10.1016/S0006-291X(89)80215-3
10.1016/0003-2697(87)90021-2
10.1016/0014-4835(92)90058-Z
10.1128/MCB.9.3.1083
10.1016/0888-7543(90)90204-8
10.1002/jnr.490280306
10.1002/glia.440050407
10.1016/0378-1119(93)90574-M
10.1016/0378-1119(93)90575-N
10.1002/dvg.1020140204
{'key': 'e_1_2_1_16_1', 'first-page': '933', 'article-title': 'Rosenthal fibers contain ubiquitinated αB‐crystallin', 'volume': '139', 'author': 'Goldman J. E.', 'year': '1991', 'journal-title': 'Am. J. Pathol.'} / Am. J. Pathol. / Rosenthal fibers contain ubiquitinated αB‐crystallin by Goldman J. E. (1991)
{'key': 'e_1_2_1_17_1', 'first-page': '363', 'article-title': 'Localization of δ‐crystallin RNA during lens morphogenesis and differentiation in the normal and Talpia3 chick embryo', 'volume': '36', 'author': 'Head M. W.', 'year': '1992', 'journal-title': 'Int. J. Dev. Biol.'} / Int. J. Dev. Biol. / Localization of δ‐crystallin RNA during lens morphogenesis and differentiation in the normal and Talpia3 chick embryo by Head M. W. (1992)
{'key': 'e_1_2_1_18_1', 'first-page': '1743', 'article-title': 'Overexpression and abnormal modification of the stress proteins αB‐crystallin and hsp27 in Alexander disease', 'volume': '143', 'author': 'Head M. W.', 'year': '1993', 'journal-title': 'Am J. Pathol.'} / Am J. Pathol. / Overexpression and abnormal modification of the stress proteins αB‐crystallin and hsp27 in Alexander disease by Head M. W. (1993)
10.1093/nar/14.10.4127
10.1016/0378-1119(86)90018-1
10.1073/pnas.89.21.10449
10.1016/0006-291X(92)91809-5
10.1073/pnas.79.7.2360
10.1016/0092-8674(89)90173-6
10.1177/38.1.2294148
10.1016/S0021-9258(18)45689-X / J. Biol. Chem. / Multiple mRNAs of rat brain α‐crystallin B chain results from alternative transcriptional initiation by Iwaki A. (1990)
10.1002/1097-0142(19911115)68:10<2230::AID-CNCR2820681023>3.0.CO;2-7
{'key': 'e_1_2_1_28_1', 'first-page': '345', 'article-title': 'Accumulation of αB‐crystallin in central nervous system glia and neurons in pathologic conditions', 'volume': '140', 'author': 'Iwaki T.', 'year': '1992', 'journal-title': 'Am. J. Pathol.'} / Am. J. Pathol. / Accumulation of αB‐crystallin in central nervous system glia and neurons in pathologic conditions by Iwaki T. (1992)
{'key': 'e_1_2_1_29_1', 'first-page': '487', 'article-title': 'αB‐crystallin and 27 kilodalton heat shock protein are regulated by stress conditions in the central nervous system and accumulate in Rosenthal fibers', 'volume': '143', 'author': 'Iwaki T.', 'year': '1993', 'journal-title': 'Am. J. Pathol.'} / Am. J. Pathol. / αB‐crystallin and 27 kilodalton heat shock protein are regulated by stress conditions in the central nervous system and accumulate in Rosenthal fibers by Iwaki T. (1993)
10.1016/S0021-9258(18)53882-5 / J. Biol. Chem. / Small heat shock proteins are molecular chaperones by Jakob U. (1993)
10.1016/0167-4838(91)90146-Q
10.1016/S0021-9258(18)42574-4 / J. Biol. Chem. / Copurification of small heat shock protein with αB‐crystallin from human skeletal muscle by Kato K. (1992)
10.1016/0167-4889(93)90214-A
10.1007/BF00713535
10.1111/j.1365-2990.1992.tb00795.x
10.1126/science.7156978
10.1073/pnas.88.9.3652
10.1083/jcb.120.3.639
{'key': 'e_1_2_1_39_1', 'first-page': '2457', 'article-title': 'Synthesis and degradation of heat shock proteins during development and decay of thermotolerance', 'volume': '42', 'author': 'Landry J.', 'year': '1982', 'journal-title': 'Cancer Res.'} / Cancer Res. / Synthesis and degradation of heat shock proteins during development and decay of thermotolerance by Landry J. (1982)
10.1083/jcb.109.1.7
10.1002/jcp.1041470113
10.1016/S0021-9258(18)53711-X / J. Biol. Chem. / Induction of Chinese hamster HSP27 gene expression in mouse cells confers resistance to heat shock by Lavoie J. (1993)
10.1002/jcp.1041500302
{'key': 'e_1_2_1_44_1', 'first-page': '309', 'volume-title': 'Culturing Nerve Cells', 'author': 'Levison S. W.', 'year': '1991'} / Culturing Nerve Cells by Levison S. W. (1991)
{'key': 'e_1_2_1_45_1', 'first-page': '2352', 'article-title': 'The effects of hypertonicity on aldose reductase, AlphaB‐crystallin and organic osmolytes in retinal pigmented epithelial', 'volume': '34', 'author': 'Lin L‐R.', 'year': '1993', 'journal-title': 'Invest. Ophthalmol. Vis. Sci.'} / Invest. Ophthalmol. Vis. Sci. / The effects of hypertonicity on aldose reductase, AlphaB‐crystallin and organic osmolytes in retinal pigmented epithelial by Lin L‐R. (1993)
10.1016/S0021-9258(18)54039-4 / J. Biol. Chem. / Structural and functional similarities of bovine α‐crystallin and mouse small heat‐shock protein by Merck K. B. (1993)
10.1016/S0021-9258(18)41621-3
10.1007/BF00276930
10.1016/S0021-9258(18)42826-8 / J. Biol. Chem. / Lens crystallins: Innovation associated with changes in gene regulation by Piatigorsky J. (1992)
10.1007/BF00160214
10.1073/pnas.82.17.5819
10.1016/0092-8674(92)90553-O
10.1016/0092-8674(91)90452-5
10.1016/S0021-9258(18)50096-X / J. Biol. Chem. / αA‐crystallin is expressed in non‐ocular tissues by Srinivasan A. N. (1992)
10.1002/dvg.1020140205
10.1016/0014-5793(92)81075-W
10.1152/physrev.1992.72.4.1063
{'key': 'e_1_2_1_58_1', 'first-page': '139', 'volume-title': 'Nucleic Acid Hybridisation', 'author': 'Williams J. G.', 'year': '1985'} / Nucleic Acid Hybridisation by Williams J. G. (1985)
10.1016/0014-5793(85)81102-9
10.1006/exer.1993.1090
10.1146/annurev.bi.57.070188.002403
10.1007/BF00227718
{'key': 'e_1_2_1_63_1', 'first-page': '379', 'volume-title': 'Stress Proteins in Biology and Medicine', 'author': 'Yost H. J.', 'year': '1990'} / Stress Proteins in Biology and Medicine by Yost H. J. (1990)
10.1016/S0021-9258(18)42364-2 / J. Biol. Chem. / Heat shock protein 27 and αB‐crystallin can form a complex, which dissociates by heat shock by Zantema A. (1992)

Dates

Type	When
Created	20 years, 6 months ago (Feb. 25, 2005, 11:35 p.m.)
Deposited	1 year, 10 months ago (Oct. 23, 2023, 7:23 p.m.)
Indexed	5 months ago (March 30, 2025, 1:42 p.m.)
Issued	31 years, 4 months ago (April 1, 1994)
Published	31 years, 4 months ago (April 1, 1994)
Published Online	20 years, 6 months ago (Feb. 4, 2005)
Published Print	31 years, 4 months ago (April 1, 1994)

Funders 0

None

BibTeX

@article{Head_1994, title={Coordinate and independent regulation of αB‐crystallin and HSP27 expression in response to physiological stress}, volume={159}, ISSN={1097-4652}, url={http://dx.doi.org/10.1002/jcp.1041590107}, DOI={10.1002/jcp.1041590107}, number={1}, journal={Journal of Cellular Physiology}, publisher={Wiley}, author={Head, Mark W. and Corbin, Elaine and Goldman, James E.}, year={1994}, month=apr, pages={41–50} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        3,
        30
      ]
    ],
    "date-time": "2025-03-30T17:42:58Z",
    "timestamp": 1743356578389
  },
  "reference-count": 63,
  "publisher": "Wiley",
  "issue": "1",
  "license": [
    {
      "start": {
        "date-parts": [
          [
            2005,
            2,
            4
          ]
        ],
        "date-time": "2005-02-04T00:00:00Z",
        "timestamp": 1107475200000
      },
      "content-version": "vor",
      "delay-in-days": 3962,
      "URL": "http://onlinelibrary.wiley.com/termsAndConditions#vor"
    }
  ],
  "content-domain": {
    "domain": [],
    "crossmark-restriction": false
  },
  "published-print": {
    "date-parts": [
      [
        1994,
        4
      ]
    ]
  },
  "abstract": "<jats:title>Abstract</jats:title><jats:p>\u03b1\u2010Crystallins share structural and functional properties with the stress protein hsp27. These polypeptides are expressed at low constitutive levels in many tissues including brain, and \u03b1B\u2010crystallin and hsp27 can accumulate in central nervous system glia in a variety of neurological conditions. We report here that heat shock and exposure to transition metals result in an increase in the steady state mRNA level of \u03b1B\u2010crystallin and hsp27 in primary cultures of rat forebrain astrocytes. Both exposure to tumour necrosis factor\u2010\u03b1 and hypertonic conditions result in \u03b1B\u2010crystallin mRNA accumulation but no change in the hsp27 mRNA level. Under some of these conditions increased synthesis and accumulation of \u03b1B\u2010crystallin and hsp27 protein are also evident. We are unable to detect \u03b1A\u2010crystallin mRNA in resting or stressed astrocytes. A novel phenomenon involving a transitory change in stress protein mRNA mobility in Northern blots during induction is reported, which is stress type and cell type independent. The results demonstrate multiple stress regulation of \u03b1B\u2010crystallin and hsp27 in cultured astrocytes, suggesting that they can legitimately be regarded as stress proteins in the central nervous system. \u00a9 1994 wiley\u2010Liss, Inc.</jats:p>",
  "DOI": "10.1002/jcp.1041590107",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2005,
        2,
        26
      ]
    ],
    "date-time": "2005-02-26T04:35:59Z",
    "timestamp": 1109392559000
  },
  "page": "41-50",
  "source": "Crossref",
  "is-referenced-by-count": 95,
  "title": "Coordinate and independent regulation of \u03b1B\u2010crystallin and HSP27 expression in response to physiological stress",
  "prefix": "10.1002",
  "volume": "159",
  "author": [
    {
      "given": "Mark W.",
      "family": "Head",
      "sequence": "first",
      "affiliation": []
    },
    {
      "given": "Elaine",
      "family": "Corbin",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "James E.",
      "family": "Goldman",
      "sequence": "additional",
      "affiliation": []
    }
  ],
  "member": "311",
  "published-online": {
    "date-parts": [
      [
        2005,
        2,
        4
      ]
    ]
  },
  "reference": [
    {
      "key": "e_1_2_1_2_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1093/nar/18.20.6049"
    },
    {
      "key": "e_1_2_1_3_1",
      "first-page": "73",
      "volume-title": "Nucleic Acid Hybridisation",
      "author": "Anderson M. L. M.",
      "year": "1985"
    },
    {
      "key": "e_1_2_1_4_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/MCB.13.3.1824"
    },
    {
      "key": "e_1_2_1_5_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/MCB.8.12.5059"
    },
    {
      "key": "e_1_2_1_6_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0006-291X(89)80215-3"
    },
    {
      "key": "e_1_2_1_7_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0003-2697(87)90021-2"
    },
    {
      "key": "e_1_2_1_8_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0014-4835(92)90058-Z"
    },
    {
      "key": "e_1_2_1_9_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/MCB.9.3.1083"
    },
    {
      "key": "e_1_2_1_10_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0888-7543(90)90204-8"
    },
    {
      "key": "e_1_2_1_11_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/jnr.490280306"
    },
    {
      "key": "e_1_2_1_12_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/glia.440050407"
    },
    {
      "key": "e_1_2_1_13_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0378-1119(93)90574-M"
    },
    {
      "key": "e_1_2_1_14_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0378-1119(93)90575-N"
    },
    {
      "key": "e_1_2_1_15_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/dvg.1020140204"
    },
    {
      "key": "e_1_2_1_16_1",
      "first-page": "933",
      "article-title": "Rosenthal fibers contain ubiquitinated \u03b1B\u2010crystallin",
      "volume": "139",
      "author": "Goldman J. E.",
      "year": "1991",
      "journal-title": "Am. J. Pathol."
    },
    {
      "key": "e_1_2_1_17_1",
      "first-page": "363",
      "article-title": "Localization of \u03b4\u2010crystallin RNA during lens morphogenesis and differentiation in the normal and Talpia3 chick embryo",
      "volume": "36",
      "author": "Head M. W.",
      "year": "1992",
      "journal-title": "Int. J. Dev. Biol."
    },
    {
      "key": "e_1_2_1_18_1",
      "first-page": "1743",
      "article-title": "Overexpression and abnormal modification of the stress proteins \u03b1B\u2010crystallin and hsp27 in Alexander disease",
      "volume": "143",
      "author": "Head M. W.",
      "year": "1993",
      "journal-title": "Am J. Pathol."
    },
    {
      "key": "e_1_2_1_19_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1093/nar/14.10.4127"
    },
    {
      "key": "e_1_2_1_20_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0378-1119(86)90018-1"
    },
    {
      "key": "e_1_2_1_21_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.89.21.10449"
    },
    {
      "key": "e_1_2_1_22_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0006-291X(92)91809-5"
    },
    {
      "key": "e_1_2_1_23_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.79.7.2360"
    },
    {
      "key": "e_1_2_1_24_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(89)90173-6"
    },
    {
      "key": "e_1_2_1_25_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1177/38.1.2294148"
    },
    {
      "key": "e_1_2_1_26_1",
      "doi-asserted-by": "crossref",
      "first-page": "22197",
      "DOI": "10.1016/S0021-9258(18)45689-X",
      "article-title": "Multiple mRNAs of rat brain \u03b1\u2010crystallin B chain results from alternative transcriptional initiation",
      "volume": "265",
      "author": "Iwaki A.",
      "year": "1990",
      "journal-title": "J. Biol. Chem."
    },
    {
      "key": "e_1_2_1_27_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/1097-0142(19911115)68:10<2230::AID-CNCR2820681023>3.0.CO;2-7"
    },
    {
      "key": "e_1_2_1_28_1",
      "first-page": "345",
      "article-title": "Accumulation of \u03b1B\u2010crystallin in central nervous system glia and neurons in pathologic conditions",
      "volume": "140",
      "author": "Iwaki T.",
      "year": "1992",
      "journal-title": "Am. J. Pathol."
    },
    {
      "key": "e_1_2_1_29_1",
      "first-page": "487",
      "article-title": "\u03b1B\u2010crystallin and 27 kilodalton heat shock protein are regulated by stress conditions in the central nervous system and accumulate in Rosenthal fibers",
      "volume": "143",
      "author": "Iwaki T.",
      "year": "1993",
      "journal-title": "Am. J. Pathol."
    },
    {
      "key": "e_1_2_1_30_1",
      "doi-asserted-by": "crossref",
      "first-page": "1517",
      "DOI": "10.1016/S0021-9258(18)53882-5",
      "article-title": "Small heat shock proteins are molecular chaperones",
      "volume": "25",
      "author": "Jakob U.",
      "year": "1993",
      "journal-title": "J. Biol. Chem."
    },
    {
      "key": "e_1_2_1_31_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0167-4838(91)90146-Q"
    },
    {
      "key": "e_1_2_1_32_1",
      "doi-asserted-by": "crossref",
      "first-page": "7718",
      "DOI": "10.1016/S0021-9258(18)42574-4",
      "article-title": "Copurification of small heat shock protein with \u03b1B\u2010crystallin from human skeletal muscle",
      "volume": "267",
      "author": "Kato K.",
      "year": "1992",
      "journal-title": "J. Biol. Chem."
    },
    {
      "key": "e_1_2_1_33_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0167-4889(93)90214-A"
    },
    {
      "key": "e_1_2_1_34_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1007/BF00713535"
    },
    {
      "key": "e_1_2_1_35_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1365-2990.1992.tb00795.x"
    },
    {
      "key": "e_1_2_1_36_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.7156978"
    },
    {
      "key": "e_1_2_1_37_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.88.9.3652"
    },
    {
      "key": "e_1_2_1_38_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1083/jcb.120.3.639"
    },
    {
      "key": "e_1_2_1_39_1",
      "first-page": "2457",
      "article-title": "Synthesis and degradation of heat shock proteins during development and decay of thermotolerance",
      "volume": "42",
      "author": "Landry J.",
      "year": "1982",
      "journal-title": "Cancer Res."
    },
    {
      "key": "e_1_2_1_40_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1083/jcb.109.1.7"
    },
    {
      "key": "e_1_2_1_41_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/jcp.1041470113"
    },
    {
      "key": "e_1_2_1_42_1",
      "doi-asserted-by": "crossref",
      "first-page": "3420",
      "DOI": "10.1016/S0021-9258(18)53711-X",
      "article-title": "Induction of Chinese hamster HSP27 gene expression in mouse cells confers resistance to heat shock",
      "volume": "268",
      "author": "Lavoie J.",
      "year": "1993",
      "journal-title": "J. Biol. Chem."
    },
    {
      "key": "e_1_2_1_43_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/jcp.1041500302"
    },
    {
      "key": "e_1_2_1_44_1",
      "first-page": "309",
      "volume-title": "Culturing Nerve Cells",
      "author": "Levison S. W.",
      "year": "1991"
    },
    {
      "key": "e_1_2_1_45_1",
      "first-page": "2352",
      "article-title": "The effects of hypertonicity on aldose reductase, AlphaB\u2010crystallin and organic osmolytes in retinal pigmented epithelial",
      "volume": "34",
      "author": "Lin L\u2010R.",
      "year": "1993",
      "journal-title": "Invest. Ophthalmol. Vis. Sci."
    },
    {
      "key": "e_1_2_1_46_1",
      "doi-asserted-by": "crossref",
      "first-page": "1046",
      "DOI": "10.1016/S0021-9258(18)54039-4",
      "article-title": "Structural and functional similarities of bovine \u03b1\u2010crystallin and mouse small heat\u2010shock protein",
      "volume": "268",
      "author": "Merck K. B.",
      "year": "1993",
      "journal-title": "J. Biol. Chem."
    },
    {
      "key": "e_1_2_1_47_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0021-9258(18)41621-3"
    },
    {
      "key": "e_1_2_1_48_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1007/BF00276930"
    },
    {
      "key": "e_1_2_1_49_1",
      "doi-asserted-by": "crossref",
      "first-page": "4277",
      "DOI": "10.1016/S0021-9258(18)42826-8",
      "article-title": "Lens crystallins: Innovation associated with changes in gene regulation",
      "volume": "4267",
      "author": "Piatigorsky J.",
      "year": "1992",
      "journal-title": "J. Biol. Chem."
    },
    {
      "key": "e_1_2_1_50_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1007/BF00160214"
    },
    {
      "key": "e_1_2_1_51_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.82.17.5819"
    },
    {
      "key": "e_1_2_1_52_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(92)90553-O"
    },
    {
      "key": "e_1_2_1_53_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(91)90452-5"
    },
    {
      "key": "e_1_2_1_54_1",
      "doi-asserted-by": "crossref",
      "first-page": "23337",
      "DOI": "10.1016/S0021-9258(18)50096-X",
      "article-title": "\u03b1A\u2010crystallin is expressed in non\u2010ocular tissues",
      "volume": "267",
      "author": "Srinivasan A. N.",
      "year": "1992",
      "journal-title": "J. Biol. Chem."
    },
    {
      "key": "e_1_2_1_55_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/dvg.1020140205"
    },
    {
      "key": "e_1_2_1_56_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0014-5793(92)81075-W"
    },
    {
      "key": "e_1_2_1_57_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1152/physrev.1992.72.4.1063"
    },
    {
      "key": "e_1_2_1_58_1",
      "first-page": "139",
      "volume-title": "Nucleic Acid Hybridisation",
      "author": "Williams J. G.",
      "year": "1985"
    },
    {
      "key": "e_1_2_1_59_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0014-5793(85)81102-9"
    },
    {
      "key": "e_1_2_1_60_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1006/exer.1993.1090"
    },
    {
      "key": "e_1_2_1_61_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1146/annurev.bi.57.070188.002403"
    },
    {
      "key": "e_1_2_1_62_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1007/BF00227718"
    },
    {
      "key": "e_1_2_1_63_1",
      "first-page": "379",
      "volume-title": "Stress Proteins in Biology and Medicine",
      "author": "Yost H. J.",
      "year": "1990"
    },
    {
      "key": "e_1_2_1_64_1",
      "doi-asserted-by": "crossref",
      "first-page": "12936",
      "DOI": "10.1016/S0021-9258(18)42364-2",
      "article-title": "Heat shock protein 27 and \u03b1B\u2010crystallin can form a complex, which dissociates by heat shock",
      "volume": "267",
      "author": "Zantema A.",
      "year": "1992",
      "journal-title": "J. Biol. Chem."
    }
  ],
  "container-title": "Journal of Cellular Physiology",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fjcp.1041590107",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "text-mining"
    },
    {
      "URL": "https://onlinelibrary.wiley.com/doi/pdf/10.1002/jcp.1041590107",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2023,
        10,
        23
      ]
    ],
    "date-time": "2023-10-23T23:23:26Z",
    "timestamp": 1698103406000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://onlinelibrary.wiley.com/doi/10.1002/jcp.1041590107"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1994,
        4
      ]
    ]
  },
  "references-count": 63,
  "journal-issue": {
    "issue": "1",
    "published-print": {
      "date-parts": [
        [
          1994,
          4
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1002/jcp.1041590107"
  ],
  "URL": "http://dx.doi.org/10.1002/jcp.1041590107",
  "relation": {},
  "ISSN": [
    "0021-9541",
    "1097-4652"
  ],
  "subject": [],
  "container-title-short": "Journal Cellular Physiology",
  "published": {
    "date-parts": [
      [
        1994,
        4
      ]
    ]
  }
}