DOI: 10.1002/jcp.1041570209. Maturation‐dependent regulation of protein kinase C activity by vitamin D<sub>3</sub> metabolites in chondrocyte cultures

Maturation‐dependent regulation of protein kinase C activity by vitamin D3 metabolites in chondrocyte cultures

10.1002/jcp.1041570209

Crossref journal-article

Wiley

Journal of Cellular Physiology (311)

Abstract

AbstractVitamin D3 metabolites regulate the differentiation of chondrocytes isolated from the growth zone or resting zone of rat costochondral cartilage. Since some of the direct membrane effects of vitamin D metabolites are nongenomic, we hypothesized that protein kinase C (PKC) plays a role in signal transduction for these chondrocyte differentiation factors and that the regulation of PKC by the vitamin D metabolites is cell maturation dependent. Confluent, fourth passage cultures of growth zone and resting zone chondrocytes were treated with vitamin D3 metabolites for up to 24 h, lysed, and cell extracts assayed for kinase activity using a specific PKC substrate peptide. The addition of 1,25‐(OH)2D3 to growth zone cell cultures resulted in a rapid dose‐dependent stimulation of PKC, significant at 10−9‐10−7 M, beginning at 3 min and sustained until 90 min; 1,25‐(OH)2D3 had no effect on PKC activity in resting zone chondrocyte cultures. The addition of 24,25‐(OH)2D3 to resting zone cultures showed a slower PKC activation, with significant stimulation seen at 90–360 min for 10−8‐10−7 M 24,25‐(OH)2D3. However, 24,25‐(OH)2D3 had no effect on PKC activity in growth zone cell cultures at all times and concentrations examined. The specificity of PKC stimulation by the vitamin D3 metabolites was verified using a specific pseudosubstrate region peptide inhibitor, which reduced PKC activity when included in the reaction mixture. Pretreatment of the cultures with U73,122, a phospholipase C inhibitor, decreased 1,25‐(OH)2D3—stimulated PKC activity but had no effect upon 24,25‐(OH)2D3–induced activity. The tyrosine kinase inhibitor, genistein, did not inhibit the PKC response in either vitamin D3 metabolites‐treated culture. Neither actinomycin D nor cycloheximide affected 1,25‐(OH)2D3–induced PKC activity in growth zone chondrocyte cultures, while both compounds inhibited 24,25‐(OH)2D3—indiuced activity in resting zone chondrocyte cultures. The results of this study indicate that vitamin D metabolites stimulate PKC activity in a metabolite‐ and cell‐maturation‐specific manner. Effects of 1,25‐(OH)2D3 appear to be nongenomic, whereas the effects of 24,25‐(OH)2D3 probably involve a genomic mechanism. © 1993 Wiley‐Liss, Inc.

Bibliography

Sylvia, V. L., Schwartz, Z., Schuman, L., Morgan, R. T., Mackey, S., Gomez, R., & Boyan, B. D. (1993). Maturationâdependent regulation of protein kinase C activity by vitamin D3 metabolites in chondrocyte cultures. Journal of Cellular Physiology, 157(2), 271â278. Portico.

Authors 7

V. L. Sylvia (first)
Z. Schwartz (additional)
L. Schuman (additional)
R. T. Morgan (additional)
S. Mackey (additional)
R. Gomez (additional)
B. D. Boyan (additional)

References 35 Referenced 85

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Dates

Type	When
Created	20 years, 6 months ago (Feb. 26, 2005, 12:35 a.m.)
Deposited	1 year, 10 months ago (Oct. 25, 2023, 7:07 a.m.)
Indexed	9 months, 1 week ago (Nov. 19, 2024, 10:53 a.m.)
Issued	31 years, 9 months ago (Nov. 1, 1993)
Published	31 years, 9 months ago (Nov. 1, 1993)
Published Online	20 years, 6 months ago (Feb. 4, 2005)
Published Print	31 years, 9 months ago (Nov. 1, 1993)

Funders 0

None

BibTeX

@article{Sylvia_1993, title={Maturation‐dependent regulation of protein kinase C activity by vitamin D3 metabolites in chondrocyte cultures}, volume={157}, ISSN={1097-4652}, url={http://dx.doi.org/10.1002/jcp.1041570209}, DOI={10.1002/jcp.1041570209}, number={2}, journal={Journal of Cellular Physiology}, publisher={Wiley}, author={Sylvia, V. L. and Schwartz, Z. and Schuman, L. and Morgan, R. T. and Mackey, S. and Gomez, R. and Boyan, B. D.}, year={1993}, month=nov, pages={271–278} }

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