DOI: 10.1002/jcp.1041510318. 1,25‐dihydroxy‐vitamin‐D<sub>3</sub> enhances antiproliferative effect and transcription of TGF‐β1 on human keratinocytes in culture

1,25‐dihydroxy‐vitamin‐D3 enhances antiproliferative effect and transcription of TGF‐β1 on human keratinocytes in culture

10.1002/jcp.1041510318

Crossref journal-article

Wiley

Journal of Cellular Physiology (311)

Abstract

AbstractBoth TGF‐β and 1,25‐dihydroxy‐vitamin‐D3 (1,25(OH)2D3) have been reported to decrease the proliferation of normal human keratinocytes. The effect and expression of TGF‐β in keratinocytes treated with 1,25(OH)2D3 was investigated. Human keratinocytes were grown in the presence of various concentrations of TGF‐β and/or 1,25(OH)2D3 prior to enumeration. TGF‐β, alone, has a half maximal dose of inhibition (ED50) of approximately 750 pg/ml after seven days in culture in Keratinocyte Growth Medium (KGM®; Clonetics) supplemented with 1.5 mM calcium. When 1,25(OH)2D3 (10−7M) was also added to cultures with various concentrations of TGF‐β, the ED50 shifted an average of 2‐fold less. The presence of TGF‐β (10 pg/ml) augmented the potency of 1,25(OH)2D3 by at least 10‐fold. In keratinocyte cultures, the antiproliferative effect of the two compounds together is synergistic. In keratinocytes grown for 1 week in the presence of 1,25(OH)2D3 at 10−6M, the TGF‐β1 message increased approximately 5‐fold. An increase is detected within 2 hours of exposure to 1,25(OH)2D3. There was only a 50% increase in the levels of TGF‐β2 and no detection of TGF‐β3. When keratinocyte cultures were treated with 1,25(OH)2D3 and neutralizing antibodies to TGF‐β, the induced‐antiproliferative activity was blocked by more than 50%. The keratinocytes produced more active than latent TGF‐β after growth with high doses of 1,25(OH)2D3. © 1992 Wiley‐Liss, Inc.

Bibliography

Kim, H., Abdelkader, N., Katz, M., & McLane, J. A. (1992). 1,25âdihydroxyâvitaminâD3 enhances antiproliferative effect and transcription of TGFâÎ²1 on human keratinocytes in culture. Journal of Cellular Physiology, 151(3), 579â587. Portico.

Authors 4

Hee‐Jong Kim (first)
Neamatallah Abdelkader (additional)
Marion Katz (additional)
John A. McLane (additional)

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Dates

Type	When
Created	20 years, 6 months ago (Feb. 26, 2005, 3:07 a.m.)
Deposited	1 year, 10 months ago (Sept. 27, 2023, 12:18 p.m.)
Indexed	4 months, 1 week ago (April 19, 2025, 4:48 a.m.)
Issued	33 years, 2 months ago (June 1, 1992)
Published	33 years, 2 months ago (June 1, 1992)
Published Online	20 years, 6 months ago (Feb. 4, 2005)
Published Print	33 years, 2 months ago (June 1, 1992)

Funders 0

None

BibTeX

@article{Kim_1992, title={1,25‐dihydroxy‐vitamin‐D3 enhances antiproliferative effect and transcription of TGF‐β1 on human keratinocytes in culture}, volume={151}, ISSN={1097-4652}, url={http://dx.doi.org/10.1002/jcp.1041510318}, DOI={10.1002/jcp.1041510318}, number={3}, journal={Journal of Cellular Physiology}, publisher={Wiley}, author={Kim, Hee‐Jong and Abdelkader, Neamatallah and Katz, Marion and McLane, John A.}, year={1992}, month=jun, pages={579–587} }

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