DOI: 10.1002/jcp.1041430103. Extracellular calcium affects the membrane currents of cultured human keratinocytes

Extracellular calcium affects the membrane currents of cultured human keratinocytes

10.1002/jcp.1041430103

Crossref journal-article

Wiley

Journal of Cellular Physiology (311)

Abstract

AbstractElectrophysiologic properties of cultured human keratinocytes were studied using the patch voltage‐clamp technique. Undifferentiated, proliferative keratinocytes grown in low Ca2+ medium had an average resting membrane potential of −24 mV. Voltage‐clamp experiments showed that these cells had two membrane ionic currents: a large voltage‐independent leak conductance, and a smaller voltage‐dependent Cl− current that activated with depolarization. Increasing the extra‐cellular Ca2+ concentration from 0.15 to 2 mM resulted in a doubling of the magnitude of the voltage‐gated current and a shift in current activation to more negative potentials. Since levels of extracellular Ca2+ can alter the morphology and differentiation state of keratinocytes, the finding of a Ca2+ ‐activated Cl− current in these cells suggests a role for this conductance in the initiation of differentiation.

Bibliography

Mauro, T. M., Pappone, P. A., & Isseroff, R. R. (1990). Extracellular calcium affects the membrane currents of cultured human keratinocytes. Journal of Cellular Physiology, 143(1), 13â20. Portico.

Authors 3

Theodora M. Mauro (first)
Pamela A. Pappone (additional)
R. Rivkah Isseroff (additional)

References 45 Referenced 32

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Dates

Type	When
Created	20 years, 6 months ago (Feb. 26, 2005, 4:58 a.m.)
Deposited	1 year, 10 months ago (Oct. 22, 2023, 4:39 p.m.)
Indexed	1 month, 3 weeks ago (July 2, 2025, 2:46 p.m.)
Issued	35 years, 4 months ago (April 1, 1990)
Published	35 years, 4 months ago (April 1, 1990)
Published Online	20 years, 6 months ago (Feb. 4, 2005)
Published Print	35 years, 4 months ago (April 1, 1990)

Funders 0

None

BibTeX

@article{Mauro_1990, title={Extracellular calcium affects the membrane currents of cultured human keratinocytes}, volume={143}, ISSN={1097-4652}, url={http://dx.doi.org/10.1002/jcp.1041430103}, DOI={10.1002/jcp.1041430103}, number={1}, journal={Journal of Cellular Physiology}, publisher={Wiley}, author={Mauro, Theodora M. and Pappone, Pamela A. and Isseroff, R. Rivkah}, year={1990}, month=apr, pages={13–20} }

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