DOI: 10.1002/jcp.1041370216. Specific binding of basic fibroblast growth factor to basement membrane‐like structures and to purified heparan sulfate proteoglycan of the EHS tumor

Specific binding of basic fibroblast growth factor to basement membrane‐like structures and to purified heparan sulfate proteoglycan of the EHS tumor

10.1002/jcp.1041370216

Crossref journal-article

Wiley

Journal of Cellular Physiology (311)

Abstract

AbstractThe binding of iodinated basic fibroblast growth factor (bFGF) to low‐density heparan sulfate proteoglycan purified from the Engelbreth Holm Swarm (EHS) sarcoma was investigated using different techniques. The tumor clearly contained bFGF, the level being comparable to that found in other tissues such as human or bovine brain. 125I bFGF strongly bound to the basement membrane‐like matrix of EHS frozen sections as revealed by autoradiography. Iodinated bFGF bound to purified heparan sulfate proteoglycan but not to laminin or collagen type IV, three components isolated from the same tumor. In contrast, acidic fibroblast growth factor (aFGF) displayed negligible binding to heparan sulfate proteoglycan. Binding of bFGF to frozen sections and to purified proteoglycan could be strongly inhibited by heparin and was displaced by an excess of unlabeled factor and completely suppressed after heparitinase and heparinase treatments. Binding was a function of the salt concentration and was abolished at 0.6 M NaCl. Scatchard analysis indicated the affinity site had a Kd of about 30 nM, a value 10–15 higher than that recently reported by Moscatelli (J. Cell. Physiol., 131:123–130, 1987) in the case of the low‐affinity binding sites present on the surface of baby hamster kidney (BHK) cells.

Bibliography

Vigny, M., OllierâHartmann, M. P., Lavigne, M., Fayein, N., Jeanny, J. C., Laurent, M., & Courtois, Y. (1988). Specific binding of basic fibroblast growth factor to basement membraneâlike structures and to purified heparan sulfate proteoglycan of the EHS tumor. Journal of Cellular Physiology, 137(2), 321â328. Portico.

Authors 7

M. Vigny (first)
M. P. Ollier‐Hartmann (additional)
M. Lavigne (additional)
N. Fayein (additional)
J. C. Jeanny (additional)
M. Laurent (additional)
Y. Courtois (additional)

References 23 Referenced 135

10.1016/0006-291X(85)90942-8
10.1042/bj2300665
10.1016/0045-6039(86)90021-7
10.1016/S0021-9258(17)39569-8
{'key': 'e_1_2_1_6_1', 'first-page': '393', 'article-title': 'A heparin‐binding angiogenic protein—bFGF—is stored within basement membrane', 'volume': '130', 'author': 'Folkman J.', 'year': '1988', 'journal-title': 'Am. J. Pathol.'} / Am. J. Pathol. / A heparin‐binding angiogenic protein—bFGF—is stored within basement membrane by Folkman J. (1988)
10.1016/0014-4827(87)90251-5
10.1016/0014-4827(83)90382-8
10.1016/S0021-9258(17)39570-4 / J. Biol. Chem. / Identification of the precursor protein to basement membrane heparan sulfate proteoglycans by Ledbetter S. R. (1985)
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Dates

Type	When
Created	20 years, 5 months ago (Feb. 26, 2005, 5:57 a.m.)
Deposited	1 year, 10 months ago (Oct. 22, 2023, 11:08 a.m.)
Indexed	1 year, 1 month ago (June 26, 2024, 10:29 a.m.)
Issued	36 years, 9 months ago (Nov. 1, 1988)
Published	36 years, 9 months ago (Nov. 1, 1988)
Published Online	20 years, 6 months ago (Feb. 4, 2005)
Published Print	36 years, 9 months ago (Nov. 1, 1988)

Funders 0

None

BibTeX

@article{Vigny_1988, title={Specific binding of basic fibroblast growth factor to basement membrane‐like structures and to purified heparan sulfate proteoglycan of the EHS tumor}, volume={137}, ISSN={1097-4652}, url={http://dx.doi.org/10.1002/jcp.1041370216}, DOI={10.1002/jcp.1041370216}, number={2}, journal={Journal of Cellular Physiology}, publisher={Wiley}, author={Vigny, M. and Ollier‐Hartmann, M. P. and Lavigne, M. and Fayein, N. and Jeanny, J. C. and Laurent, M. and Courtois, Y.}, year={1988}, month=nov, pages={321–328} }

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