DOI: 10.1002/jcp.1040990314. Control of the Balb/c‐3T3 cell cycle by nutrients and serum factors: Analysis using platelet‐derived growth factor and platelet‐poor plasma

Control of the Balb/c‐3T3 cell cycle by nutrients and serum factors: Analysis using platelet‐derived growth factor and platelet‐poor plasma

10.1002/jcp.1040990314

Crossref journal-article

Wiley

Journal of Cellular Physiology (311)

Abstract

AbstractMuch controversy regarding the relationship between nutrients and serum in regulation of cell growth can be reconciled by recognizing that serum contains multiple factors which regulate different events in the cell cycle. Serum was fractioned into a platelet‐derived growth factor (PDGF), which induces cells to become competent to synthesize DNA, and plasma which allows competent cells to traverse G0/G1 and enter the S phase. Nutrients are not required for the cellular response to PDGF; however amino acids are required for plasma to promote the entry of PDGF‐treated, competent cells into S phase. The nutrient independent, PDGF‐modulated, growth regulatory event (competence) is located 12 hours prior to the G1/S phase boundary in quiescent, density‐arrested Balb/c‐3T3 cells. The nutrient dependent, plasma‐modulated event is located six hours prior to the G1/S phase boundary and corresponds in time to a plasma dependent growth arrest point. Moreover, plasma controls the concentration of amino acids required for DNA synthesis. Infection of density‐arrested Balb/c‐3T3 cells with SV40 overrides both the nutrient independent and the nutrient dependent growth regulatory events.

Bibliography

Stiles, C. D., Isberg, R. R., Pledger, W. J., Antoniades, H. N., & Scher, C. D. (1979). Control of the Balb/câ3T3 cell cycle by nutrients and serum factors: Analysis using plateletâderived growth factor and plateletâpoor plasma. Journal of Cellular Physiology, 99(3), 395â405. Portico.

Authors 5

C. D. Stiles (first)
R. R. Isberg (additional)
W. J. Pledger (additional)
H. N. Antoniades (additional)
C. D. Scher (additional)

References 42 Referenced 87

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{'key': 'e_1_2_1_29_1', 'first-page': '541', 'article-title': 'Different survival of normal and transformed cells exposed to nutritional conditions nonpermissive for growth', 'volume': '37', 'author': 'Schiaffonati L.', 'year': '1977', 'journal-title': 'Cancer Res.'} / Cancer Res. / Different survival of normal and transformed cells exposed to nutritional conditions nonpermissive for growth by Schiaffonati L. (1977)
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{'key': 'e_1_2_1_35_1', 'first-page': '15a', 'article-title': 'Control of the Bald/c‐3T3 cell cycle by nutrients and serum factors: analysis using platelet derived growth factor and platelet‐poor plasma', 'volume': '79', 'author': 'Stiles C. D.', 'year': '1978', 'journal-title': 'J. Cell. Biol.'} / J. Cell. Biol. / Control of the Bald/c‐3T3 cell cycle by nutrients and serum factors: analysis using platelet derived growth factor and platelet‐poor plasma by Stiles C. D. (1978)
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Dates

Type	When
Created	20 years, 5 months ago (Feb. 26, 2005, 11:18 a.m.)
Deposited	1 year, 9 months ago (Nov. 11, 2023, 10:56 p.m.)
Indexed	2 days, 15 hours ago (Aug. 23, 2025, 1:01 a.m.)
Issued	46 years, 2 months ago (June 1, 1979)
Published	46 years, 2 months ago (June 1, 1979)
Published Online	20 years, 6 months ago (Feb. 4, 2005)
Published Print	46 years, 2 months ago (June 1, 1979)

Funders 0

None

BibTeX

@article{Stiles_1979, title={Control of the Balb/c‐3T3 cell cycle by nutrients and serum factors: Analysis using platelet‐derived growth factor and platelet‐poor plasma}, volume={99}, ISSN={1097-4652}, url={http://dx.doi.org/10.1002/jcp.1040990314}, DOI={10.1002/jcp.1040990314}, number={3}, journal={Journal of Cellular Physiology}, publisher={Wiley}, author={Stiles, C. D. and Isberg, R. R. and Pledger, W. J. and Antoniades, H. N. and Scher, C. D.}, year={1979}, month=jun, pages={395–405} }

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