DOI: 10.1002/jcp.1040900302. Growth and biochemical characteristics of a detachment variant of CHO cells

Growth and biochemical characteristics of a detachment variant of CHO cells

10.1002/jcp.1040900302

Crossref journal-article

Wiley

Journal of Cellular Physiology (311)

Abstract

AbstractA variant subline of Chinese hamster cells (line CHO) was isolated that had increased resistance to detachment from the substratum. Comparisons between parental and variant cells of the complex carbohydrates liberated during trypsin detachment showed that the variant cells synthesized little or no hyaluronic acid. These cells also had reduced amounts of other complex carbohydrates in the cell periphery. However, parental and variant cells did not differ in morphology, growth control, or cyclic AMP concentration. Profound changes in the physical and chemical nature of the cell periphery, in themselves, evidently are insufficient to cause changes in many aspects of cell behavior.

Bibliography

Atherly, A. G., Barnhart, B. J., & Kraemer, P. M. (1977). Growth and biochemical characteristics of a detachment variant of CHO cells. Journal of Cellular Physiology, 90(3), 375â385. Portico.

Authors 3

Alan G. Atherly (first)
Benjamin J. Barnhart (additional)
Paul M. Kraemer (additional)

References 38 Referenced 48

{'key': 'e_1_2_1_2_1', 'first-page': '2382', 'article-title': 'Properties of acidic saccharides produced by B16 melanoma cells treated with 1‐methyl‐3‐isobutylxanthine', 'volume': '35', 'author': 'Banks J.', 'year': '1975', 'journal-title': 'Cancer Res.'} / Cancer Res. / Properties of acidic saccharides produced by B16 melanoma cells treated with 1‐methyl‐3‐isobutylxanthine by Banks J. (1975)
10.1126/science.1114343
10.1073/pnas.48.1.41
10.1038/252501a0
10.1021/bi00761a024
{'key': 'e_1_2_1_7_1', 'first-page': '179', 'volume-title': 'Methods in Cell Biology', 'author': 'Deaven L. L.', 'year': '1974'} / Methods in Cell Biology by Deaven L. L. (1974)
10.1073/pnas.68.9.2165
10.1016/S0021-9258(19)44824-2 / J. Biol. Chem. / The effect of dibutyryl cyclic adenosine monophosphate on synthesis of sulfated acid mucopolysaccharides by transformed fibroblasts by Goggins J. F. (1972)
{'key': 'e_1_2_1_10_1', 'first-page': '517', 'volume-title': 'Control of Proliferation in Animal Cells', 'author': 'Grimes W. J.', 'year': '1974'} / Control of Proliferation in Animal Cells by Grimes W. J. (1974)
10.1016/S0014-4827(63)80014-2
{'key': 'e_1_2_1_12_1', 'first-page': '19', 'volume-title': 'Cellular Recognition', 'author': 'Holtzer H.', 'year': '1968'} / Cellular Recognition by Holtzer H. (1968)
10.1002/path.1700930112
10.1083/jcb.62.3.881
10.1038/newbio236247a0 / Nature New Biol. / Cyclic AMP increases the adhesion of fibroblasts to substratum by Johnson G. S. (1972)
10.1007/978-1-4684-3327-2_2
10.1021/bi00784a026
{'key': 'e_1_2_1_18_1', 'first-page': '372', 'volume-title': 'Growth, Nutrition, and Metabolism in Cells in Culture', 'author': 'Kraemer P. M.', 'year': '1972'} / Growth, Nutrition, and Metabolism in Cells in Culture by Kraemer P. M. (1972)
{'key': 'e_1_2_1_19_1', 'volume-title': 'Surfaces of Normal, and Malignant Cells', 'author': 'Kraemer P. M.', 'year': '1977'} / Surfaces of Normal, and Malignant Cells by Kraemer P. M. (1977)
{'key': 'e_1_2_1_20_1', 'first-page': '703', 'volume-title': 'Chemistry, and Molecular Biology of the Intracellular Matrix', 'author': 'Laurent T. C.', 'year': '1970'} / Chemistry, and Molecular Biology of the Intracellular Matrix by Laurent T. C. (1970)
10.1038/219033a0
10.1038/254695a0
10.1073/pnas.71.6.2310
10.1073/pnas.69.8.2069
10.1016/S0006-291X(71)80212-7
10.1073/pnas.73.2.544
10.1021/bi00673a022
10.1016/0092-8674(74)90034-8
10.1016/0092-8674(74)90133-0
10.1002/ijc.2910030517
10.1038/246200a0
10.1016/0014-4827(71)90590-8
10.1021/bi00700a004
10.1084/jem.108.2.259
10.1126/science.169570
10.1016/0012-1606(72)90071-1
10.1073/pnas.69.6.1384
10.1002/jcp.1040750307
10.1101/SQB.1974.039.01.050

Dates

Type	When
Created	20 years, 6 months ago (Feb. 26, 2005, 12:31 p.m.)
Deposited	1 year, 9 months ago (Nov. 12, 2023, 3:03 a.m.)
Indexed	1 day, 13 hours ago (Sept. 4, 2025, 9:11 a.m.)
Issued	48 years, 6 months ago (March 1, 1977)
Published	48 years, 6 months ago (March 1, 1977)
Published Online	20 years, 7 months ago (Feb. 4, 2005)
Published Print	48 years, 6 months ago (March 1, 1977)

Funders 0

None

BibTeX

@article{Atherly_1977, title={Growth and biochemical characteristics of a detachment variant of CHO cells}, volume={90}, ISSN={1097-4652}, url={http://dx.doi.org/10.1002/jcp.1040900302}, DOI={10.1002/jcp.1040900302}, number={3}, journal={Journal of Cellular Physiology}, publisher={Wiley}, author={Atherly, Alan G. and Barnhart, Benjamin J. and Kraemer, Paul M.}, year={1977}, month=mar, pages={375–385} }

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