DOI: 10.1002/jcp.1040890210. Unidirectional K<sup>+</sup> fluxes in rat thymocytes stimulated by concanavalin A

Unidirectional K+ fluxes in rat thymocytes stimulated by concanavalin A

10.1002/jcp.1040890210

Crossref journal-article

Wiley

Journal of Cellular Physiology (311)

Abstract

AbstractUnidirectional K+ fluxes were estimated in isolated rat thymocytes by 42K exchange kinetics. The cells were either preloaded with isotope and the release of it measured during incubation for one hour at 38°C, or the cellular uptake of isotope during a similar incubation was measured.The influx rate of untreated thymocytes was: 2.3·10−12 moles cm −2. S−1 and efflux rate: 1.8·10−12 moles cm −2· S−1·When con A was added to the cells, influx was raised 74% and efflux 65%. Maximal effect was obtained when the concentration of con A was 15 μg/ml, but concentrations as low as 0.75 μg/ml were effective.Hydrocortisone resistant thymocytes responded at least as well as untreated cells to con A, which also raised RNA synthesis rate in the former cells 2.5 times.Using an extracellular marker, 51CrEDTA, intracellular concentrations of some ions was estimated in the thymocytes after one hour incubation: Na+: 30 mmoles/kg water, K+: 177 mmoles/kg water and Cl−: 43 mmoles/kg water. Cellular water content: 69%. These values were not found significantly altered when con A was present.Since con A raised influx and efflux to the same extent and no net flux of K+ could be detected, it is proposed that both active and passive transport of K+ was increased by con A.The increased fluxes induced by con A, can apparently not be reversed by removal of con A from the incubation medium or by addition of the inhibiting hapten, α‐methyl‐D‐mannoside.

Bibliography

Iversen, J. (1976). Unidirectional K+ fluxes in rat thymocytes stimulated by concanavalin A. Journal of Cellular Physiology, 89(2), 267â276. Portico.

Authors 1

Jens‐Gustav Iversen (first)

References 21 Referenced 30

{'key': 'e_1_2_1_2_1', 'first-page': '509', 'article-title': 'The early uptake of radioactive calcium by human lymphocytes treated with phytohaemagglutinin', 'volume': '21', 'author': 'Allwood G.', 'year': '1971', 'journal-title': 'Immunology'} / Immunology / The early uptake of radioactive calcium by human lymphocytes treated with phytohaemagglutinin by Allwood G. (1971)
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10.1007/978-1-4613-4413-1_6
10.1016/0304-4157(75)90002-7
10.1002/jcp.1040800309
{'key': 'e_1_2_1_9_1', 'volume-title': 'Lymphocyte stimulation', 'author': 'Ling N. R.', 'year': '1975'} / Lymphocyte stimulation by Ling N. R. (1975)
10.1038/251324b0
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{'key': 'e_1_2_1_17_1', 'first-page': '1034', 'article-title': 'Accelerated potassium accumulation by lectin‐stimulated lymphocytes: A reassessment', 'volume': '46', 'author': 'Segel G. B.', 'year': '1975', 'journal-title': 'Blood'} / Blood / Accelerated potassium accumulation by lectin‐stimulated lymphocytes: A reassessment by Segel G. B. (1975)
{'key': 'e_1_2_1_18_1', 'first-page': '167', 'article-title': 'Equilibrium density gradient separation and analysis of lymphocyte populations', 'volume': '2', 'author': 'Shortman K.', 'year': '1969', 'journal-title': 'Progr. Separ. Purif.'} / Progr. Separ. Purif. / Equilibrium density gradient separation and analysis of lymphocyte populations by Shortman K. (1969)
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10.1002/jcp.1040800303

Dates

Type	When
Created	20 years, 5 months ago (Feb. 26, 2005, 12:26 p.m.)
Deposited	1 year, 10 months ago (Oct. 19, 2023, 4:54 a.m.)
Indexed	1 year, 2 months ago (June 17, 2024, 4:29 p.m.)
Issued	48 years, 10 months ago (Oct. 1, 1976)
Published	48 years, 10 months ago (Oct. 1, 1976)
Published Online	20 years, 6 months ago (Feb. 4, 2005)
Published Print	48 years, 10 months ago (Oct. 1, 1976)

Funders 0

None

BibTeX

@article{Iversen_1976, title={Unidirectional K+ fluxes in rat thymocytes stimulated by concanavalin A}, volume={89}, ISSN={1097-4652}, url={http://dx.doi.org/10.1002/jcp.1040890210}, DOI={10.1002/jcp.1040890210}, number={2}, journal={Journal of Cellular Physiology}, publisher={Wiley}, author={Iversen, Jens‐Gustav}, year={1976}, month=oct, pages={267–276} }

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