DOI: 10.1002/jcp.1040840106. A mammalian somatic “cell cycle” mutant defective in G<sub>1</sub>

A mammalian somatic “cell cycle” mutant defective in G1

10.1002/jcp.1040840106

Crossref journal-article

Wiley

Journal of Cellular Physiology (311)

Abstract

AbstractVariants or “mutants” temperature‐sensitive (ts) for growth have been isolated by selection from a near‐diploid mouse cell line. Thus far. 10 ts mutants which grow normally at 33° C, but not at 39° C, have been isolated. These ts mutants were then studied to determine if any manifested their defect at a unique point or stage in the cell cycle. This type of ts mutant is termed a “cell cycle” mutant.The first screen involves observing individual cells of an asynchronous culture for residual division after a shift from 33° C (permissive temperature) to 39° (nonpermissive temperature). A cell cycle mutant should show some fraction of the cells dividing only once at a normal rate after the shift. The ts variant B54 met this first criterion for a cell cycle mutant (i.e., 50% residual division) and was further analyzed.The second screening technique monitors (1) the rate of entry into S, (2) the length of G2, and (3) the rate and duration of cells entering mitosis after a shift of an asynchronous culture to 39°. This experiment with B54 revealed that cells in G1 at the time of the shift to 39° failed to enter S while cells already into S completed the cycle at 39°. These results suggest that B54 is defective in a G1 function which is required for entry into S, but which is no longer needed once cells have entered S. Other results are presented which also support this hypothesis.In addition the ts function of B54 is apparently required for recovery from a “high density” G1 arrest.

Bibliography

Michael Liskay, R. (1974). A mammalian somatic âcell cycleâ mutant defective in G1. Journal of Cellular Physiology, 84(1), 49â55. Portico.

Authors 1

R. Michael Liskay (first)

References 23 Referenced 46

{'key': 'e_1_2_1_2_1', 'first-page': '167', 'article-title': 'Biochemistry of the cell cycle; a review', 'volume': '1', 'author': 'Baserga R.', 'year': '1968', 'journal-title': 'Cell Tissue Kinet.'} / Cell Tissue Kinet. / Biochemistry of the cell cycle; a review by Baserga R. (1968)
{'key': 'e_1_2_1_3_1', 'first-page': '75', 'article-title': 'On the resting periods in the cell life cycle', 'volume': '2', 'author': 'Epifanova O. I.', 'year': '1969', 'journal-title': 'Cell Tissue Kinet.'} / Cell Tissue Kinet. / On the resting periods in the cell life cycle by Epifanova O. I. (1969)
Farber R. A. andR. M.Liskay1974Cytogenetics in press.
10.1093/genetics/74.2.267 / Genetics / Cenetic control of the cell division cycle in yeast by Hartwell L. H. (1973)
10.1083/jcb.57.3.760
{'issue': '1', 'key': 'e_1_2_1_7_1', 'first-page': '143', 'article-title': 'On the concept of the cell cycle', 'volume': '62', 'author': 'Lajtha L. G.', 'year': '1963', 'journal-title': 'J. Cell. Physiol.'} / J. Cell. Physiol. / On the concept of the cell cycle by Lajtha L. G. (1963)
10.1038/newbio239066a0
{'key': 'e_1_2_1_9_1', 'first-page': '21', 'volume-title': 'The biology of the cell cycle', 'author': 'Witchison J. M.', 'year': '1971'} / The biology of the cell cycle by Witchison J. M. (1971)
{'key': 'e_1_2_1_10_1', 'first-page': '269', 'volume-title': 'Biochemistry of Disease', 'author': 'Mueller G. C.', 'year': '1971'} / Biochemistry of Disease by Mueller G. C. (1971)
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10.1152/physrev.1963.43.3.357
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10.1016/0027-5107(73)90259-5
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Wang R.1974Nature in press.

Dates

Type	When
Created	20 years, 6 months ago (Feb. 26, 2005, 12:48 p.m.)
Deposited	1 year, 9 months ago (Nov. 12, 2023, 5:47 a.m.)
Indexed	1 month, 3 weeks ago (July 11, 2025, 6:15 a.m.)
Issued	51 years, 1 month ago (Aug. 1, 1974)
Published	51 years, 1 month ago (Aug. 1, 1974)
Published Online	20 years, 7 months ago (Feb. 4, 2005)
Published Print	51 years, 1 month ago (Aug. 1, 1974)

Funders 0

None

BibTeX

@article{Michael_Liskay_1974, title={A mammalian somatic “cell cycle” mutant defective in G1}, volume={84}, ISSN={1097-4652}, url={http://dx.doi.org/10.1002/jcp.1040840106}, DOI={10.1002/jcp.1040840106}, number={1}, journal={Journal of Cellular Physiology}, publisher={Wiley}, author={Michael Liskay, R.}, year={1974}, month=aug, pages={49–55} }

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