DOI: 10.1002/jcp.1040740211. Early alterations in amino acid pools and protein synthesis of diploid fibroblasts stimulated to synthesize DNA by addition of serum

Early alterations in amino acid pools and protein synthesis of diploid fibroblasts stimulated to synthesize DNA by addition of serum

10.1002/jcp.1040740211

Crossref journal-article

Wiley

Journal of Cellular Physiology (311)

Abstract

AbstractDiploid human fibroblasts in culture (WI 38) were allowed to reach a stationary phase and were then stimulated to reenter DNA synthesis and cell division by addition of serum to the culture medium. The rate of protein synthesis increased during the first hours after addition of serum reaching at three hours a plateau value that continued for at least 24 hours after serum addition. Inhibition of protein synthesis during the early hours after serum addition abolished the stimulation of DNA synthesis occurring 20 to 28 hours later.Increased protein synthesis was preceded by a rapid decrease in the intracellular pool size of most amino acids. These changes were independent of concomitant protein synthesis. They suggest that serum exerts an immediate effect on the function of the cell membrane.

Bibliography

Wiebel, F., & Baserga, R. (1969). Early alterations in amino acid pools and protein synthesis of diploid fibroblasts stimulated to synthesize DNA by addition of serum. Journal of Cellular Physiology, 74(2), 191â202. Portico.

Authors 2

F. Wiebel (first)
R. Baserga (additional)

References 43 Referenced 117

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{'key': 'e_1_2_1_5_1', 'first-page': '167', 'article-title': 'Biochemistry of the cell cycle: a review', 'volume': '1', 'author': 'Baserga R.', 'year': '1968', 'journal-title': 'Cell Tissue Kin.'} / Cell Tissue Kin. / Biochemistry of the cell cycle: a review by Baserga R. (1968)
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{'key': 'e_1_2_1_8_1', 'first-page': '602', 'article-title': 'Effects of Ehrlich ascites antisera on the biochemical activities of Ehrlich ascites carcinoma cells in vitro', 'volume': '19', 'author': 'Bickis I. J.', 'year': '1959', 'journal-title': 'Cancer Res.'} / Cancer Res. / Effects of Ehrlich ascites antisera on the biochemical activities of Ehrlich ascites carcinoma cells in vitro by Bickis I. J. (1959)
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{'issue': '2', 'key': 'e_1_2_1_18_1', 'first-page': '644', 'article-title': 'Early alteration in phospholipid metabolism of lymphocytes by phytohemagglutinin (PHA)', 'volume': '27', 'author': 'Fisher D.', 'year': '1968', 'journal-title': 'Fed. Proc.'} / Fed. Proc. / Early alteration in phospholipid metabolism of lymphocytes by phytohemagglutinin (PHA) by Fisher D. (1968)
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10.1016/S0021-9258(18)97497-1 / J. Biol. Chem. / Liver nuclear ribonucleic acid polymerase formed after partial hepatectomy by Tsukada K. (1965)
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Dates

Type	When
Created	20 years, 6 months ago (Feb. 26, 2005, 1:25 p.m.)
Deposited	1 year, 9 months ago (Nov. 12, 2023, 9:45 a.m.)
Indexed	1 year, 9 months ago (Nov. 12, 2023, 10:10 a.m.)
Issued	55 years, 11 months ago (Oct. 1, 1969)
Published	55 years, 11 months ago (Oct. 1, 1969)
Published Online	20 years, 6 months ago (Feb. 4, 2005)
Published Print	55 years, 11 months ago (Oct. 1, 1969)

Funders 0

None

BibTeX

@article{Wiebel_1969, title={Early alterations in amino acid pools and protein synthesis of diploid fibroblasts stimulated to synthesize DNA by addition of serum}, volume={74}, ISSN={1097-4652}, url={http://dx.doi.org/10.1002/jcp.1040740211}, DOI={10.1002/jcp.1040740211}, number={2}, journal={Journal of Cellular Physiology}, publisher={Wiley}, author={Wiebel, F. and Baserga, R.}, year={1969}, month=oct, pages={191–202} }

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