DOI: 10.1002/jcp.1040690111. Stimulation by normal and leukemic mouse sera of colony formation <i>in vitro</i> by mouse bone marrow cells

Stimulation by normal and leukemic mouse sera of colony formation in vitro by mouse bone marrow cells

10.1002/jcp.1040690111

Crossref journal-article

Wiley

Journal of Cellular Physiology (311)

Abstract

AbstractUsing a modification of the agar gel method for bone marrow culture, serum from various strains of mice has been tested for colony stimulating activity. Ninety percent of sera from AKR mice with spontaneous or transplanted lymphoid leukemia and 40–50% of sera from normal or preleukemic AKR mice stimulated colony formation by C57B1 bone marrow cells. Sera from 6% of C3H and 30% of C57B1 mice stimulated similar colony formation. The incidence of sera with colony stimulating activity rose with increasing age. All colonies were initially mainly granulocytic in nature but later became pure populations of mononuclear cells.Bone marrow cells exhibited considerable variation in their responsiveness to stimulation by mouse serum. Increasing the serum dose increased the number and size of bone marrow cell colonies and with optimal serum doses, 1 in 1000 bone marrow cells formed a cell colony. Preincubation of cells with active serum did not stimulate colony formation by washed bone marrow cells.The active factor in serum was filterable, non‐dialysable and heat and ether labile.

Bibliography

Robinson, W., Metcalf, D., & Bradley, T. R. (1967). Stimulation by normal and leukemic mouse sera of colony formation in vitro by mouse bone marrow cells. Journal of Cellular Physiology, 69(1), 83â91. Portico.

Authors 3

W. Robinson (first)
D. Metcalf (additional)
T. R. Bradley (additional)

References 9 Referenced 148

10.1038/icb.1966.28
Bradley T. R. D. MetcalfandW.Robinson1966Stimulation by leukemic sera of colony formation in solid agar cultures by proliferation of mouse bone marrow cells.Nature (in press). (10.1038/213926a0)
{'key': 'e_1_2_1_4_1', 'first-page': '551', 'article-title': 'Studies of thymic function with emphasis on the role of the thymus in oncogenesis', 'volume': '26', 'author': 'Law L. W.', 'year': '1966', 'journal-title': 'Cancer Res.'} / Cancer Res. / Studies of thymic function with emphasis on the role of the thymus in oncogenesis by Law L. W. (1966)
10.1038/149439a0
{'key': 'e_1_2_1_6_1', 'first-page': '1774', 'article-title': 'The fate of parental preleukemic cells in leukemia‐susceptible and leukemia‐resistant F1 hybrid mice', 'volume': '23', 'author': 'Metcalf D.', 'year': '1963', 'journal-title': 'Cancer Res.'} / Cancer Res. / The fate of parental preleukemic cells in leukemia‐susceptible and leukemia‐resistant F1 hybrid mice by Metcalf D. (1963)
{'key': 'e_1_2_1_7_1', 'first-page': '425', 'article-title': 'Histological and transplantation studies on the preleukemic AKR mouse thymus', 'volume': '37', 'author': 'Metcalf D.', 'year': '1966', 'journal-title': 'J. Nat. Cancer Instit.'} / J. Nat. Cancer Instit. / Histological and transplantation studies on the preleukemic AKR mouse thymus by Metcalf D. (1966)
Metcalf D. T. R. BradleyandW.Robinson1966Analysis of colonies developing in agar from mouse bone marrow cells stimulated by kidney feeder layers or leukemic serum.J. Cell. Comp. Physiol.(in press). (10.1002/jcp.1040690112)
10.1002/jcp.1030660309
Pluznik D. H. andL.Sachs1966The induction of clones of normal “mast” cells by a substance from conditioned medium.Exptl. Cell. Res.(in press). (10.1016/0014-4827(66)90026-7)

Dates

Type	When
Created	20 years, 6 months ago (Feb. 25, 2005, 10:48 p.m.)
Deposited	1 year, 9 months ago (Nov. 12, 2023, 7:43 a.m.)
Indexed	14 hours, 45 minutes ago (Sept. 4, 2025, 9:29 a.m.)
Issued	58 years, 7 months ago (Feb. 1, 1967)
Published	58 years, 7 months ago (Feb. 1, 1967)
Published Online	20 years, 7 months ago (Feb. 4, 2005)
Published Print	58 years, 7 months ago (Feb. 1, 1967)

Funders 0

None

BibTeX

@article{Robinson_1967, title={Stimulation by normal and leukemic mouse sera of colony formation in vitro by mouse bone marrow cells}, volume={69}, ISSN={1097-4652}, url={http://dx.doi.org/10.1002/jcp.1040690111}, DOI={10.1002/jcp.1040690111}, number={1}, journal={Journal of Cellular Physiology}, publisher={Wiley}, author={Robinson, W. and Metcalf, D. and Bradley, T. R.}, year={1967}, month=feb, pages={83–91} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        9,
        4
      ]
    ],
    "date-time": "2025-09-04T13:29:39Z",
    "timestamp": 1756992579218
  },
  "reference-count": 9,
  "publisher": "Wiley",
  "issue": "1",
  "license": [
    {
      "start": {
        "date-parts": [
          [
            2005,
            2,
            4
          ]
        ],
        "date-time": "2005-02-04T00:00:00Z",
        "timestamp": 1107475200000
      },
      "content-version": "vor",
      "delay-in-days": 13883,
      "URL": "http://onlinelibrary.wiley.com/termsAndConditions#vor"
    }
  ],
  "content-domain": {
    "domain": [],
    "crossmark-restriction": false
  },
  "published-print": {
    "date-parts": [
      [
        1967,
        2
      ]
    ]
  },
  "abstract": "<jats:title>Abstract</jats:title><jats:p>Using a modification of the agar gel method for bone marrow culture, serum from various strains of mice has been tested for colony stimulating activity. Ninety percent of sera from AKR mice with spontaneous or transplanted lymphoid leukemia and 40\u201350% of sera from normal or preleukemic AKR mice stimulated colony formation by C57B1 bone marrow cells. Sera from 6% of C3H and 30% of C57B1 mice stimulated similar colony formation. The incidence of sera with colony stimulating activity rose with increasing age. All colonies were initially mainly granulocytic in nature but later became pure populations of mononuclear cells.</jats:p><jats:p>Bone marrow cells exhibited considerable variation in their responsiveness to stimulation by mouse serum. Increasing the serum dose increased the number and size of bone marrow cell colonies and with optimal serum doses, 1 in 1000 bone marrow cells formed a cell colony. Preincubation of cells with active serum did not stimulate colony formation by washed bone marrow cells.</jats:p><jats:p>The active factor in serum was filterable, non\u2010dialysable and heat and ether labile.</jats:p>",
  "DOI": "10.1002/jcp.1040690111",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2005,
        2,
        26
      ]
    ],
    "date-time": "2005-02-26T03:48:28Z",
    "timestamp": 1109389708000
  },
  "page": "83-91",
  "source": "Crossref",
  "is-referenced-by-count": 148,
  "title": "Stimulation by normal and leukemic mouse sera of colony formation <i>in vitro</i> by mouse bone marrow cells",
  "prefix": "10.1002",
  "volume": "69",
  "author": [
    {
      "given": "W.",
      "family": "Robinson",
      "sequence": "first",
      "affiliation": []
    },
    {
      "given": "D.",
      "family": "Metcalf",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "T. R.",
      "family": "Bradley",
      "sequence": "additional",
      "affiliation": []
    }
  ],
  "member": "311",
  "published-online": {
    "date-parts": [
      [
        2005,
        2,
        4
      ]
    ]
  },
  "reference": [
    {
      "key": "e_1_2_1_2_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/icb.1966.28"
    },
    {
      "key": "e_1_2_1_3_1",
      "doi-asserted-by": "crossref",
      "unstructured": "Bradley T. R. D. MetcalfandW.Robinson1966Stimulation by leukemic sera of colony formation in solid agar cultures by proliferation of mouse bone marrow cells.Nature (in press).",
      "DOI": "10.1038/213926a0"
    },
    {
      "key": "e_1_2_1_4_1",
      "first-page": "551",
      "article-title": "Studies of thymic function with emphasis on the role of the thymus in oncogenesis",
      "volume": "26",
      "author": "Law L. W.",
      "year": "1966",
      "journal-title": "Cancer Res."
    },
    {
      "key": "e_1_2_1_5_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/149439a0"
    },
    {
      "key": "e_1_2_1_6_1",
      "first-page": "1774",
      "article-title": "The fate of parental preleukemic cells in leukemia\u2010susceptible and leukemia\u2010resistant F1 hybrid mice",
      "volume": "23",
      "author": "Metcalf D.",
      "year": "1963",
      "journal-title": "Cancer Res."
    },
    {
      "key": "e_1_2_1_7_1",
      "first-page": "425",
      "article-title": "Histological and transplantation studies on the preleukemic AKR mouse thymus",
      "volume": "37",
      "author": "Metcalf D.",
      "year": "1966",
      "journal-title": "J. Nat. Cancer Instit."
    },
    {
      "key": "e_1_2_1_8_1",
      "doi-asserted-by": "crossref",
      "unstructured": "Metcalf D. T. R. BradleyandW.Robinson1966Analysis of colonies developing in agar from mouse bone marrow cells stimulated by kidney feeder layers or leukemic serum.J. Cell. Comp. Physiol.(in press).",
      "DOI": "10.1002/jcp.1040690112"
    },
    {
      "key": "e_1_2_1_9_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/jcp.1030660309"
    },
    {
      "key": "e_1_2_1_10_1",
      "doi-asserted-by": "crossref",
      "unstructured": "Pluznik D. H. andL.Sachs1966The induction of clones of normal \u201cmast\u201d cells by a substance from conditioned medium.Exptl. Cell. Res.(in press).",
      "DOI": "10.1016/0014-4827(66)90026-7"
    }
  ],
  "container-title": "Journal of Cellular Physiology",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fjcp.1040690111",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "text-mining"
    },
    {
      "URL": "https://onlinelibrary.wiley.com/doi/pdf/10.1002/jcp.1040690111",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2023,
        11,
        12
      ]
    ],
    "date-time": "2023-11-12T12:43:11Z",
    "timestamp": 1699792991000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://onlinelibrary.wiley.com/doi/10.1002/jcp.1040690111"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1967,
        2
      ]
    ]
  },
  "references-count": 9,
  "journal-issue": {
    "issue": "1",
    "published-print": {
      "date-parts": [
        [
          1967,
          2
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1002/jcp.1040690111"
  ],
  "URL": "http://dx.doi.org/10.1002/jcp.1040690111",
  "relation": {},
  "ISSN": [
    "0021-9541",
    "1097-4652"
  ],
  "subject": [],
  "container-title-short": "Journal Cellular Physiology",
  "published": {
    "date-parts": [
      [
        1967,
        2
      ]
    ]
  }
}