DOI: 10.1002/jcp.1040680103. Potentiation of the colchicine effect on frog melanocytes by high hydrostatic pressure

Potentiation of the colchicine effect on frog melanocytes by high hydrostatic pressure

10.1002/jcp.1040680103

Crossref journal-article

Wiley

Journal of Cellular Physiology (311)

Abstract

AbstractIn dermal melanocytes of Rana pipiens, colchicine is known to produce a gradual, dosage‐dependent dispersion of melanin granules, irreversible over several hours. This effect is potentiated by a number of chemical agents that normally produce a reversible dispersion of granules. In the present study we examined the effect of high hydrostatic pressure on changes induced in melanocytes by colchicine.In Ringer's solution, samples of skin from a single frog were incubated for 30 minutes at room temperature with or without colchicine, 9 × 10−5 M. Then two samples, one of which had been pretreated with colchicine, were successively subjected to 12,000 psi for one hour at 25 to 26°C. The degree of dispersion of melanin granules in melanocytes was observed before, during and after the period of pressure.In frog skin pretreated with colchicine, the usually gradual, irreversible dispersion of melanin granules in melanocytes was potentiated.Since high pressure is known to produce solational changes in protoplasm, such changes may accompany dispersion of melanin granules in melanocytes. If this be so, then sol‐gel equilibria may be important in the action of dispersing and aggregating agents, many of which are hormones and other physiologically active agents. Finally, the present study supports the hypothesis that colchicine shifts protoplasmic sol‐gel equilibria toward a less gelated condition.

Bibliography

Malawista, S. E., Asterita, H., & Marsland, D. (1966). Potentiation of the colchicine effect on frog melanocytes by high hydrostatic pressure. Journal of Cellular Physiology, 68(1), 13â17. Portico.

Authors 3

Stephen E. Malawista (first)
Harvey Asterita (additional)
Douglas Marsland (additional)

References 19 Referenced 15

10.1002/art.1780080438
{'key': 'e_1_2_1_3_1', 'first-page': '305', 'article-title': 'The effect of colchicine on the microscopic and submicroscopic structure of the mitotic spindle', 'volume': '2', 'author': 'Inoué S.', 'year': '1952', 'journal-title': 'Exp. Cell Res., Supp.'} / Exp. Cell Res., Supp. / The effect of colchicine on the microscopic and submicroscopic structure of the mitotic spindle by Inoué S. (1952)
10.1002/jcp.1030440206
10.1038/184674a0
10.1038/jid.1959.38
10.1084/jem.122.2.361
10.1002/art.1780080437
Malawista S. E.1965cUnpublished data.
{'key': 'e_1_2_1_10_1', 'first-page': '414', 'article-title': 'Melatonin reversibly augments mitotic spindle birefringence and inhibits the colchicine effect', 'volume': '129', 'author': 'Malawista S. E.', 'year': '1965', 'journal-title': 'Biol. Bull.'} / Biol. Bull. / Melatonin reversibly augments mitotic spindle birefringence and inhibits the colchicine effect by Malawista S. E. (1965)
10.7326/0003-4819-62-4-648
10.2307/1537960
10.1002/jcp.1030360207
10.1016/S0074-7696(08)62571-1
{'key': 'e_1_2_1_15_1', 'first-page': '182', 'article-title': 'Protoplasmic contractility in relation to cytokinesis', 'volume': '28', 'author': 'Marsland D. A.', 'year': '1956', 'journal-title': 'Publ. Statz. Zool. Napoli'} / Publ. Statz. Zool. Napoli / Protoplasmic contractility in relation to cytokinesis by Marsland D. A. (1956)
{'key': 'e_1_2_1_16_1', 'first-page': '396', 'article-title': 'Pressure induced enhancement of the anti‐mitotic effects of colchicine in the cleaving eggs of Lytechinus variegatus', 'volume': '129', 'author': 'Marsland D. A.', 'year': '1965', 'journal-title': 'Biol. Bull.'} / Biol. Bull. / Pressure induced enhancement of the anti‐mitotic effects of colchicine in the cleaving eggs of Lytechinus variegatus by Marsland D. A. (1965)
10.1002/jmor.1050690302
10.2307/1538257
10.1210/endo-66-4-599
10.1016/0014-4827(64)90096-5

Dates

Type	When
Created	20 years, 6 months ago (Feb. 25, 2005, 10:48 p.m.)
Deposited	1 year, 9 months ago (Nov. 12, 2023, 7:03 a.m.)
Indexed	1 year, 7 months ago (Jan. 11, 2024, 11:39 a.m.)
Issued	59 years, 1 month ago (Aug. 1, 1966)
Published	59 years, 1 month ago (Aug. 1, 1966)
Published Online	20 years, 7 months ago (Feb. 4, 2005)
Published Print	59 years, 1 month ago (Aug. 1, 1966)

Funders 0

None

BibTeX

@article{Malawista_1966, title={Potentiation of the colchicine effect on frog melanocytes by high hydrostatic pressure}, volume={68}, ISSN={1097-4652}, url={http://dx.doi.org/10.1002/jcp.1040680103}, DOI={10.1002/jcp.1040680103}, number={1}, journal={Journal of Cellular Physiology}, publisher={Wiley}, author={Malawista, Stephen E. and Asterita, Harvey and Marsland, Douglas}, year={1966}, month=aug, pages={13–17} }

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