DOI: 10.1002/jcb.240420403. DNA‐binding activity of jun is increased through its interaction with Fos

DNA‐binding activity of jun is increased through its interaction with Fos

10.1002/jcb.240420403

Crossref journal-article

Wiley

Journal of Cellular Biochemistry (311)

Abstract

AbstractTranscription factor AP‐1 mediates induction of a set of genes in response to the phorbol ester tumor promoter TPA. Recently, AP‐I preparations from HeLa cells were shown to contain a product of the c‐JUN protooncogene (Jun/AP‐l) which forms a tight complex with the Fos protein. In this paper, we examine the role of the Fos protein in the DNA‐binding activity of the AP‐I complex. We show that the DNA‐binding activity of bacterially expressed trpE‐Jun fusion proteins is increased many‐fold upon their interaction with Fos (or a Fos‐relaied antigen) expressed from a baculovirus vector. The site of Fos interaction is within the DNA‐binding domain of Jun/AP‐l, and anti‐Fos antibodies interfere with the binding of affinity purified AP‐1 to DNA. These results suggest that, by associating with Jun/AP‐l, Fos is responsible for the formation of a multimeric protein complex that has greater affinity for the target sequence than does Jun/AP‐l alone.

Authors 5

Elizabeth A. Allegretto (first)
Tod Smeal (additional)
Peter Angel (additional)
Bruce M. Spiegelman (additional)
Michael Karin (additional)

References 36 Referenced 27

10.1016/0092-8674(87)90611-8
10.1016/0092-8674(87)90612-X
10.1038/329648a0
10.1126/science.2825349
10.1038/332166a0
10.1016/0092-8674(88)90076-1
10.1073/pnas.84.10.3316
10.1016/0092-8674(88)90408-4
10.1016/0092-8674(88)90143-2
10.1016/0092-8674(87)90621-0
10.1016/S0092-8674(88)80039-4
10.1126/science.3130660
10.1016/0092-8674(88)90195-X
10.1016/0092-8674(88)90146-8
10.1016/0092-8674(88)90147-X
10.1038/336646a0
10.1038/336692a0
10.1101/gad.2.12b.1687
{'key': 'e_1_2_1_20_2', 'volume-title': 'A Manual of Methods for Baculovirus Vectors and Insect Cell Culture Procedures', 'author': 'Summers MD', 'year': '1981'} / A Manual of Methods for Baculovirus Vectors and Insect Cell Culture Procedures by Summers MD (1981)
10.1073/pnas.76.9.4350
10.1128/MCB.5.1.167
10.1093/nar/9.23.6505
10.1016/0092-8674(88)90037-2
10.1038/325368a0
10.1128/MCB.7.6.2201
10.1016/0092-8674(85)90022-4
10.1126/science.2494700
10.1126/science.2494701
10.1126/science.2494702
10.1016/0092-8674(89)90253-5
10.1126/science.3289117
{'key': 'e_1_2_1_33_2', 'first-page': '43', 'volume': '3', 'author': 'Lucibello FC', 'year': '1988', 'journal-title': 'Oncogene'} / Oncogene by Lucibello FC (1988)
10.1016/0092-8674(87)90328-X
10.1016/0092-8674(87)90326-6
10.1016/0092-8674(87)90582-4
10.1101/SQB.1988.053.01.019

Dates

Type	When
Created	20 years, 8 months ago (Dec. 30, 2004, 10:25 a.m.)
Deposited	1 year, 10 months ago (Oct. 22, 2023, 3:41 p.m.)
Indexed	1 year, 2 months ago (June 17, 2024, 2:38 p.m.)
Issued	35 years, 5 months ago (April 1, 1990)
Published	35 years, 5 months ago (April 1, 1990)
Published Online	21 years, 6 months ago (Feb. 19, 2004)
Published Print	35 years, 5 months ago (April 1, 1990)

Funders 0

None

BibTeX

@article{Allegretto_1990, title={DNA‐binding activity of jun is increased through its interaction with Fos}, volume={42}, ISSN={1097-4644}, url={http://dx.doi.org/10.1002/jcb.240420403}, DOI={10.1002/jcb.240420403}, number={4}, journal={Journal of Cellular Biochemistry}, publisher={Wiley}, author={Allegretto, Elizabeth A. and Smeal, Tod and Angel, Peter and Spiegelman, Bruce M. and Karin, Michael}, year={1990}, month=apr, pages={193–206} }

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