DOI: 10.1002/jcb.10380. Immunohistochemical analysis of Bin1/Amphiphysin II in human tissues: Diverse sites of nuclear expression and losses in prostate cancer

Immunohistochemical analysis of Bin1/Amphiphysin II in human tissues: Diverse sites of nuclear expression and losses in prostate cancer

10.1002/jcb.10380

Crossref journal-article

Wiley

Journal of Cellular Biochemistry (311)

Abstract

AbstractThe Bin1/Amphiphysin II gene encodes at least seven alternately spliced adapter proteins that have been implicated in membrane dynamics and nuclear processes. Nuclear localized Bin1 polypeptides have tumor suppressor and proapoptotic activities, suggesting that Bin1 may suppress cancer in tissues where nuclear expression may occur. One question is the extent to which human tissues express nuclear Bin1 isoforms. A secondary issue has been the need for a specific antibody that can detect all the splice isoforms expressed by the human, mouse, and rat Bin1 genes. Using a novel mouse monoclonal antibody with these characteristics, we performed an immunohistochemical analysis of Bin1 expression in a panel of normal human tissues. We also compared the expression profile of Bin1 in normal or malignant tissues derived from human prostate, where Bin1 is a candidate tumor suppressor gene. In brain, a distinct nuclear staining pattern overlapped with a cytosolic staining pattern present in certain layers of the cerebral cortex and cerebellum. Bone marrow cells displayed mainly nuclear localization whereas peripheral lymphoid cells exhibited mainly cytosolic localization. In several epithelial tissues, nuclear or nucleocytosolic staining patterns were displayed by basal cells in skin, breast, or prostate, whereas cytosolic or plasma membrane‐associated staining patterns were noted in gastrointestinal cells. Interestingly, a striking gradient of expression was observed in gastrointestinal epithelia, particularly in the large intestine, with the strongest staining displayed by cells destined to undergo apoptosis at the villus tip. In prostate, Bin1 staining was frequently absent in cases of primary prostate adenocarcinoma. This study used a novel reagent to document the extent of expression of nuclear Bin1 isoforms, which exhibit cancer suppression and proapoptotic activity in human cells. © 2002 Wiley‐Liss, Inc.

Bibliography

DuHadaway, J. B., Lynch, F. J., Brisbay, S., BuesoâRamos, C., Troncoso, P., McDonnell, T., & Prendergast, G. C. (2002). Immunohistochemical analysis of Bin1/Amphiphysin II in human tissues: Diverse sites of nuclear expression and losses in prostate cancer. Journal of Cellular Biochemistry, 88(3), 635â642. Portico.

Authors 7

James B. DuHadaway (first)
Frank J. Lynch (additional)
Shawn Brisbay (additional)
Carlos Bueso‐Ramos (additional)
Patricia Troncoso (additional)
Timothy McDonnell (additional)
George C. Prendergast (additional)

References 20 Referenced 36

10.1083/jcb.137.6.1355
10.1073/pnas.93.1.331
{'key': 'e_1_2_4_4_1', 'first-page': '3151', 'article-title': 'Bin1 mediates apoptosis by c‐Myc in transformed primary cells', 'volume': '16', 'author': 'DuHadaway JB', 'year': '2001', 'journal-title': 'Cancer Res'} / Cancer Res / Bin1 mediates apoptosis by c‐Myc in transformed primary cells by DuHadaway JB (2001)
10.1038/sj.onc.1202670
10.1038/sj.onc.1203681
10.1073/pnas.96.17.9689
10.1002/(SICI)1097-0215(20000201)85:3<376::AID-IJC14>3.0.CO;2-1
10.1002/(SICI)1097-0215(20000415)86:2<155::AID-IJC2>3.0.CO;2-M
10.1073/pnas.94.23.12390
10.1007/BF01542654
10.1002/j.1460-2075.1992.tb05317.x
10.1046/j.1471-4159.1998.70062369.x
10.1074/jbc.272.26.16700
RouthierEL DonoverPS PrendergastGC. In presshob1+ the homolog of Bin1/Amphiphysin II in fission yeast is dispensable for endocytosis but required for starvation or genotoxic stress. Oncogene.
10.1038/ng0996-69
10.1006/bbrc.1997.6927
{'key': 'e_1_2_4_18_1', 'first-page': '3258', 'article-title': 'The putative tumor suppressor BIN1 is a short‐lived nuclear phosphoprotein whose localization is altered in malignant cells', 'volume': '57', 'author': 'Wechsler‐Reya R', 'year': '1997', 'journal-title': 'Cancer Res'} / Cancer Res / The putative tumor suppressor BIN1 is a short‐lived nuclear phosphoprotein whose localization is altered in malignant cells by Wechsler‐Reya R (1997)
10.1074/jbc.272.50.31453
10.1128/MCB.18.1.566
10.1091/mbc.8.10.2003

Dates

Type	When
Created	22 years, 7 months ago (Jan. 17, 2003, 11:46 a.m.)
Deposited	1 year, 9 months ago (Nov. 17, 2023, 9:30 a.m.)
Indexed	1 day, 1 hour ago (Aug. 31, 2025, 7:25 p.m.)
Issued	22 years, 9 months ago (Nov. 27, 2002)
Published	22 years, 9 months ago (Nov. 27, 2002)
Published Online	22 years, 9 months ago (Nov. 27, 2002)
Published Print	22 years, 6 months ago (Feb. 15, 2003)

Funders 0

None

BibTeX

@article{DuHadaway_2002, title={Immunohistochemical analysis of Bin1/Amphiphysin II in human tissues: Diverse sites of nuclear expression and losses in prostate cancer}, volume={88}, ISSN={1097-4644}, url={http://dx.doi.org/10.1002/jcb.10380}, DOI={10.1002/jcb.10380}, number={3}, journal={Journal of Cellular Biochemistry}, publisher={Wiley}, author={DuHadaway, James B. and Lynch, Frank J. and Brisbay, Shawn and Bueso‐Ramos, Carlos and Troncoso, Patricia and McDonnell, Timothy and Prendergast, George C.}, year={2002}, month=nov, pages={635–642} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        8,
        31
      ]
    ],
    "date-time": "2025-08-31T23:25:22Z",
    "timestamp": 1756682722626
  },
  "reference-count": 20,
  "publisher": "Wiley",
  "issue": "3",
  "license": [
    {
      "start": {
        "date-parts": [
          [
            2002,
            11,
            27
          ]
        ],
        "date-time": "2002-11-27T00:00:00Z",
        "timestamp": 1038355200000
      },
      "content-version": "vor",
      "delay-in-days": 0,
      "URL": "http://onlinelibrary.wiley.com/termsAndConditions#vor"
    }
  ],
  "content-domain": {
    "domain": [],
    "crossmark-restriction": false
  },
  "published-print": {
    "date-parts": [
      [
        2003,
        2,
        15
      ]
    ]
  },
  "abstract": "<jats:title>Abstract</jats:title><jats:p>The <jats:italic>Bin1</jats:italic>/<jats:italic>Amphiphysin II</jats:italic> gene encodes at least seven alternately spliced adapter proteins that have been implicated in membrane dynamics and nuclear processes. Nuclear localized Bin1 polypeptides have tumor suppressor and proapoptotic activities, suggesting that Bin1 may suppress cancer in tissues where nuclear expression may occur. One question is the extent to which human tissues express nuclear Bin1 isoforms. A secondary issue has been the need for a specific antibody that can detect all the splice isoforms expressed by the human, mouse, and rat <jats:italic>Bin1</jats:italic> genes. Using a novel mouse monoclonal antibody with these characteristics, we performed an immunohistochemical analysis of Bin1 expression in a panel of normal human tissues. We also compared the expression profile of Bin1 in normal or malignant tissues derived from human prostate, where <jats:italic>Bin1</jats:italic> is a candidate tumor suppressor gene. In brain, a distinct nuclear staining pattern overlapped with a cytosolic staining pattern present in certain layers of the cerebral cortex and cerebellum. Bone marrow cells displayed mainly nuclear localization whereas peripheral lymphoid cells exhibited mainly cytosolic localization. In several epithelial tissues, nuclear or nucleocytosolic staining patterns were displayed by basal cells in skin, breast, or prostate, whereas cytosolic or plasma membrane\u2010associated staining patterns were noted in gastrointestinal cells. Interestingly, a striking gradient of expression was observed in gastrointestinal epithelia, particularly in the large intestine, with the strongest staining displayed by cells destined to undergo apoptosis at the villus tip. In prostate, Bin1 staining was frequently absent in cases of primary prostate adenocarcinoma. This study used a novel reagent to document the extent of expression of nuclear Bin1 isoforms, which exhibit cancer suppression and proapoptotic activity in human cells. \u00a9 2002 Wiley\u2010Liss, Inc.</jats:p>",
  "DOI": "10.1002/jcb.10380",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2003,
        1,
        17
      ]
    ],
    "date-time": "2003-01-17T16:46:53Z",
    "timestamp": 1042822013000
  },
  "page": "635-642",
  "source": "Crossref",
  "is-referenced-by-count": 36,
  "title": "Immunohistochemical analysis of Bin1/Amphiphysin II in human tissues: Diverse sites of nuclear expression and losses in prostate cancer",
  "prefix": "10.1002",
  "volume": "88",
  "author": [
    {
      "given": "James B.",
      "family": "DuHadaway",
      "sequence": "first",
      "affiliation": []
    },
    {
      "given": "Frank J.",
      "family": "Lynch",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Shawn",
      "family": "Brisbay",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Carlos",
      "family": "Bueso\u2010Ramos",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Patricia",
      "family": "Troncoso",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Timothy",
      "family": "McDonnell",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "George C.",
      "family": "Prendergast",
      "sequence": "additional",
      "affiliation": []
    }
  ],
  "member": "311",
  "published-online": {
    "date-parts": [
      [
        2002,
        11,
        27
      ]
    ]
  },
  "reference": [
    {
      "key": "e_1_2_4_2_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1083/jcb.137.6.1355"
    },
    {
      "key": "e_1_2_4_3_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.93.1.331"
    },
    {
      "key": "e_1_2_4_4_1",
      "first-page": "3151",
      "article-title": "Bin1 mediates apoptosis by c\u2010Myc in transformed primary cells",
      "volume": "16",
      "author": "DuHadaway JB",
      "year": "2001",
      "journal-title": "Cancer Res"
    },
    {
      "key": "e_1_2_4_5_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/sj.onc.1202670"
    },
    {
      "key": "e_1_2_4_6_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/sj.onc.1203681"
    },
    {
      "key": "e_1_2_4_7_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.96.17.9689"
    },
    {
      "key": "e_1_2_4_8_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/(SICI)1097-0215(20000201)85:3<376::AID-IJC14>3.0.CO;2-1"
    },
    {
      "key": "e_1_2_4_9_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/(SICI)1097-0215(20000415)86:2<155::AID-IJC2>3.0.CO;2-M"
    },
    {
      "key": "e_1_2_4_10_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.94.23.12390"
    },
    {
      "key": "e_1_2_4_11_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1007/BF01542654"
    },
    {
      "key": "e_1_2_4_12_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/j.1460-2075.1992.tb05317.x"
    },
    {
      "key": "e_1_2_4_13_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1046/j.1471-4159.1998.70062369.x"
    },
    {
      "key": "e_1_2_4_14_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1074/jbc.272.26.16700"
    },
    {
      "key": "e_1_2_4_15_1",
      "unstructured": "RouthierEL DonoverPS PrendergastGC. In presshob1+ the homolog of Bin1/Amphiphysin II in fission yeast is dispensable for endocytosis but required for starvation or genotoxic stress. Oncogene."
    },
    {
      "key": "e_1_2_4_16_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/ng0996-69"
    },
    {
      "key": "e_1_2_4_17_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1006/bbrc.1997.6927"
    },
    {
      "key": "e_1_2_4_18_1",
      "first-page": "3258",
      "article-title": "The putative tumor suppressor BIN1 is a short\u2010lived nuclear phosphoprotein whose localization is altered in malignant cells",
      "volume": "57",
      "author": "Wechsler\u2010Reya R",
      "year": "1997",
      "journal-title": "Cancer Res"
    },
    {
      "key": "e_1_2_4_19_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1074/jbc.272.50.31453"
    },
    {
      "key": "e_1_2_4_20_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1128/MCB.18.1.566"
    },
    {
      "key": "e_1_2_4_21_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1091/mbc.8.10.2003"
    }
  ],
  "container-title": "Journal of Cellular Biochemistry",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://onlinelibrary.wiley.com/doi/pdf/10.1002/jcb.10380",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2023,
        11,
        17
      ]
    ],
    "date-time": "2023-11-17T14:30:22Z",
    "timestamp": 1700231422000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://onlinelibrary.wiley.com/doi/10.1002/jcb.10380"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        2002,
        11,
        27
      ]
    ]
  },
  "references-count": 20,
  "journal-issue": {
    "issue": "3",
    "published-print": {
      "date-parts": [
        [
          2003,
          2,
          15
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1002/jcb.10380"
  ],
  "URL": "http://dx.doi.org/10.1002/jcb.10380",
  "relation": {},
  "ISSN": [
    "0730-2312",
    "1097-4644"
  ],
  "subject": [],
  "container-title-short": "J of Cellular Biochemistry",
  "published": {
    "date-parts": [
      [
        2002,
        11,
        27
      ]
    ]
  }
}