DOI: 10.1002/jbmr.5650040403. One-day test using stable isotopes to measure true fractional calcium absorption

One-day test using stable isotopes to measure true fractional calcium absorption

10.1002/jbmr.5650040403

Crossref journal-article

Oxford University Press (OUP)

Journal of Bone and Mineral Research (286)

Abstract

Abstract True calcium absorption can only be measured by the time-consuming and expensive metabolic balance method and is not predicted well by measuring the fractional absorption (FA) of radiocalcium from a fixed calcium carrier. We describe here a 1 day method for measuring the actual fraction of calcium absorbed from the habitual diet (true fractional calcium absorption, TFCA) using stable isotopes of calcium. Oral and intravenous isotopes were administered with each of the three daily meals, and then the ratio of the two isotopes in the urine was measured by mass spectroscopy. In 12 subjects, TFCA determined from stable Ca correlated well with TFCA measured by the balance method (r = 0.71, P &lt; 0.01), and the mean values were not different (0.26 and 0.26). In contrast, no significant correlation was found between FA and TFCA. The weak relationship between FA and TFCA underscores the importance of tracing dietary calcium rather than a fixed calcium carrier in tests of calcium absorption. Using the new method, TFCA was inversely related to dietary calcium (r = −0.45, P &lt; 0.05), demonstrating that it could detect physiological changes in calcium absorption. Thus, this test has two important advantages: (1) it provides a simple way to measure TFCA and true fractional calcium absorption (the product of TFCA and dietary calcium), the physiologically relevant variables, and (2) because there is no radiation exposure, the test can be used in pregnant women and children, the isotopes can be prepared in advance, and several isotopes can be used simultaneously.

Bibliography

Eastell, R., Riggs, B. L., Vieira, N. E., & Yergey, A. L. (1989). One-day test using stable isotopes to measure true fractional calcium absorption. Journal of Bone and Mineral Research, 4(4), 463â468.

Authors 4

Richard Eastell (first)
B. Lawrence Riggs (additional)
Nancy E. Vieira (additional)
Alfred L. Yergey (additional)

References 16 Referenced 66

{'key': '2024012712242615600_bib1', 'first-page': '641', 'article-title': 'A simple method for measurement of intestinal calcium absorption in humans by double-isotope technique', 'volume': '105', 'author': 'Griessen', 'year': '1985', 'journal-title': 'J Lab Clin Med'} / J Lab Clin Med / A simple method for measurement of intestinal calcium absorption in humans by double-isotope technique by Griessen (1985)
10.1016/0169-6009(89)90027-5 / Bone Miner / Comparison of polyethylene glycol and chromium-51 chloride as nonabsorbable stool markers in calcium balance studies by Eastell (1989)
{'key': '2024012712242615600_bib3', 'first-page': '570', 'article-title': 'Intestinal calcium absorption: Concurrent use of oral and intravenous tracers and calculation by the inverse convolution method', 'volume': '79', 'author': 'Szymendera', 'year': '1972', 'journal-title': 'J Lab Clin Med'} / J Lab Clin Med / Intestinal calcium absorption: Concurrent use of oral and intravenous tracers and calculation by the inverse convolution method by Szymendera (1972)
10.1203/00006450-198519040-00001 / Pediatr Res / Dynamics of calcium metabolism in infancy and childhood. I. Methodology and quantification in the infant by Moore (1985)
10.1038/222146a0 / Nature / Bone turnover model based on a continuously expanding exchangeable calcium pool by Burkinshaw (1969)
10.1021/ac50062a008 / Anal Chem / Isotope ratio measurements of urinary calcium with a thermal ionization probe in a quadrupole mass spectrometer by Yergey (1980)
10.1016/0221-8747(80)90012-0 / Metab Bone Dis Relat Res / A comparison between the balance method and radiotracer methods for measuring calcium absorption in treated and untreated patients with osteoporosis by Reeve (1980)
10.1042/cs0610477 / Clin Sci / A comparison of radioactive calcium absorption tests with net calcium absorption by Marshall (1981)
10.1016/S0009-9120(73)80032-3 / Clin Biochem / An evaluation of isotopic calcium absorption tests by Harrison (1973)
10.1093/ajcn/43.2.299 / Am J Clin Nutr / Distribution of calcium absorption in middle-aged women by Heaney (1986)
10.1007/BF02013226 / Calcif Tissue Res / Use of stable 48Ca in the clinical measurement of intestinal calcium absorption by Neer (1978)
10.1007/BF01480086 / Klin Wochenschr / Bestimmung der enteralen resorption hoher calciumdosen mittels stabiler isotope by Behne (1978)
10.1210/jcem-66-2-294 / J Clin Endocrinol Metab / Absent intestinal response to calciferols in hereditary resistance to 1,25-dihydroxy-vitamin D: Documentation and effective therapy with high dose intravenous calcium infusions by Bliziotes (1988)
{'key': '2024012712242615600_bib14', 'first-page': '285', 'article-title': 'Study on the kinetics of calcium metabolism with the aid of 46Ca followed by neutron activation analysis', 'volume': '6', 'author': 'Szymendera', 'year': '1967', 'journal-title': 'Nucl Med'} / Nucl Med / Study on the kinetics of calcium metabolism with the aid of 46Ca followed by neutron activation analysis by Szymendera (1967)
10.1210/jcem-33-4-661 / J Clin Endocrinol Metab / Calcium metabolism in normal human pregnancy by Heaney (1971)
10.1021/bk-1984-0258.ch002 / Stable Isotopes in Nutrition by Yergey (1984)

Dates

Type	When
Created	15 years, 6 months ago (Feb. 3, 2010, 1:08 p.m.)
Deposited	1 year, 5 months ago (March 13, 2024, 10:50 a.m.)
Indexed	6 months, 1 week ago (Feb. 21, 2025, 8:34 a.m.)
Issued	36 years, 1 month ago (Aug. 1, 1989)
Published	36 years, 1 month ago (Aug. 1, 1989)
Published Online	36 years, 1 month ago (Aug. 1, 1989)
Published Print	36 years, 1 month ago (Aug. 1, 1989)

Funders 2

NIH
Awards1
1. AG-04875
U.S. Public Health Service 10.13039/100007197
Region: Americas
gov (National government)
Labels6
1. United States Public Health Service
2. Commissioned Corps of the U.S. Public Health Service
3. USPHS Commissioned Corps
4. U.S. Public Health Service Commissioned Corps
5. USPHS
6. PHS

BibTeX

@article{Eastell_1989, title={One-day test using stable isotopes to measure true fractional calcium absorption}, volume={4}, ISSN={1523-4681}, url={http://dx.doi.org/10.1002/jbmr.5650040403}, DOI={10.1002/jbmr.5650040403}, number={4}, journal={Journal of Bone and Mineral Research}, publisher={Oxford University Press (OUP)}, author={Eastell, Richard and Riggs, B. Lawrence and Vieira, Nancy E. and Yergey, Alfred L.}, year={1989}, month=aug, pages={463–468} }

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