DOI: 10.1002/jbmr.5650030503. Evidence of a probable role for 25-hydroxyvitamin D in the regulation of human calcium metabolism

Evidence of a probable role for 25-hydroxyvitamin D in the regulation of human calcium metabolism

10.1002/jbmr.5650030503

Crossref journal-article

Oxford University Press (OUP)

Journal of Bone and Mineral Research (286)

Abstract

Abstract 1,25-Dihydroxyvitamin D [1,25-(OH)2D] is the principal mediator of the biologic effects of vitamin D. We showed previously that obese white subjects have low serum vitamin D and 25-hydroxyvitamin D (25-OHD) with increased serum-immunoreactive parathyroid hormone (PTH) and 1,25-(OH)2D, low urinary calcium, and increased urinary cyclic adenosine 3′,5′-monophosphate (cyclic AMP) compared with nonobese white individuals. To determine whether 25-OHD modulates calcium metabolism, the effects of 25-OHD3, 40–100 μg/day for 9 days, were compared in seven obese and seven nonobese white subjects who were between the ages of 20 and 34 years. Each of them was hospitalized on a metabolic ward and given a constant daily diet that contained 400 mg calcium, 900 mg phosphate, and 18 mEq magnesium. Whereas 25-OHD3 increased mean serum 25-OHD from 7 ± 1 to 37 ± 5 ng/ml (P &lt; 0.01) and urinary calcium from 102 ± 18 to 146 ± 17 mg/day (P &lt; 0.001) and decreased mean serum 1,25-(OH)2D from 40 ± 2 to 28 ± 2 pg/ml (P &lt; 0.01) and urinary cyclic AMP from 3.23 ± 0.57 to 2.00 ± 0.17 nM/dl GF (P &lt; 0.05), it did not change mean serum calcium, ionized calcium, phosphate, magnesium, immunoreactive PTH or urinary phosphate, or creatinine clearance in the obese subjects. In contrast, 25-OHD3 increased mean serum 25-OHD from 16 ± 1 to 46 ± 4 pg/ml (P &lt; 0.001) but did not alter mean serum 1,25-(OH)2D or urinary calcium or cyclic AMP in the nonobese subjects. Metabolic balance studies carried out in two of the obese subjects showed that 25-OHD3 increased serum 25-OHD and urinary calcium and lowered serum 1,25-(OH)2D. The findings are interpreted to mean that elevated serum 1,25-(OH)2D and urinary cyclic AMP and low urinary calcium in obese subjects result from a deficiency of vitamin D and 25-OHD, that these changes are reversed by 25-OHD3, and that in humans 25-OHD has a modest role in the regulation of calcium metabolism that is independent of the regulation by 1,25-(OH)2D.

Bibliography

Dr. Bell, N. H., Epstein, S., Shary, J., Greene, V., Oexmann, M. J., & Shaw, S. (1988). Evidence of a probable role for 25-hydroxyvitamin D in the regulation of human calcium metabolism. Journal of Bone and Mineral Research, 3(5), 489â495.

Authors 6

Norman H. Dr. Bell (first)
Sol Epstein (additional)
Judith Shary (additional)
Virginia Greene (additional)
Mary Joan Oexmann (additional)
Sheryl Shaw (additional)

References 31 Referenced 48

10.1021/bi00790a023 / Biochemistry / Isolation and identification of 1,25-dihydroxycholecalciferol. A metabolite of vitamin D active in intestine. by Holick (1971)
10.1126/science.175.4023.768 / Science / 1,25-Dihydroxycholecalciferol: A potent stimulator of bone resorption in tissue culture. by Raisz (1972)
10.1007/BF02409404 / Calcif Tissue Int / A monolog on analogs: In vitro effects of vitamin D metabolites and consideration of the mineralization question. by Stern (1981)
{'key': '2024012712202713700_bib4', 'first-page': '7', 'volume-title': 'Bone and Mineral Research', 'author': 'DeLuca', 'year': '1983'} / Bone and Mineral Research by DeLuca (1983)
10.1172/JCI111930 / J Clin Invest / Vitamin D-endocrine system. by Bell (1985)
10.1210/edrv-6-4-491 / Endocrine Rev / Evidence that 1,25-dihydroxyvitamin D3 is the physiologically active metabolite of vitamin D3. by Brommage (1985)
{'key': '2024012712202713700_bib7', 'author': 'Liel'} by Liel
10.1172/JCI111971 / J Clin Invest / Evidence for alteration of the vitamin D-endocrine system in obese subjects. by Bell (1985)
{'key': '2024012712202713700_bib9', 'first-page': '49', 'article-title': 'Direct microdetermination of calcium.', 'volume': '46', 'author': 'Baginsky', 'year': '1973', 'journal-title': 'Clin Chim Acta'} / Clin Chim Acta / Direct microdetermination of calcium. by Baginsky (1973)
10.1016/S0021-9258(18)84756-1 / J Biol Chem / The colorimetric determination of phosphorus. by Fiske (1925)
10.1016/0009-8981(71)90467-0 / Clin Chem Acta / Eine mikromethode zur kreatinin bestimmung. by Bartels (1971)
{'key': '2024012712202713700_bib12', 'first-page': '662', 'article-title': 'Colorimetric determination with bound “Calmagite” of magnesium in human blood serum.', 'volume': '17', 'author': 'Gindler', 'year': '1971', 'journal-title': 'Clin Chem'} / Clin Chem / Colorimetric determination with bound “Calmagite” of magnesium in human blood serum. by Gindler (1971)
{'key': '2024012712202713700_bib13', 'first-page': '791', 'article-title': 'Importance of the isolation of 25-hydroxyvitamin D before assay.', 'volume': '91', 'author': 'Dorantes', 'year': '1978', 'journal-title': 'J Lab Clin Med'} / J Lab Clin Med / Importance of the isolation of 25-hydroxyvitamin D before assay. by Dorantes (1978)
10.1210/jcem-58-1-91 / J Clin Endocrinol Metab / A microassay for 1,25-dihydroxyvitamin D not requiring high performance liquid chromatography: Application to clinical studies. by Reinhardt (1984)
10.1002/jbmr.5650010203 / J Bone Min Res / Evidence that obesity does not influence the vitamin D-endocrine system in blacks. by Epstein (1986)
10.1073/pnas.67.1.305 / Proc Natl Acad Sci USA / A protein binding assay for adenosine 3′,5′-monophosphate. by Gilman (1970)
10.1172/JCI108831 / J Clin Invest / Nephrogenous cyclic adenosine monophosphate as a parathyroid function test. by Broadus (1977)
10.1172/JCI104673 / J Clin Invest / The effect of parathyroid extract on renal tubular calcium reabsorption in the dog. by Widrow (1962)
10.1007/978-1-4613-2839-1_5 / Vitamin D: Basic and Clinical Aspects by Henry (1984)
10.1172/JCI108459 / J Clin Invest / Radioligand receptor assay for 25-hydroxyvitamin D2/D3 and 1α,25-dihydroxyvitamin D2/D3: Application to hypervitaminosis D. by Hughes (1976)
10.1172/JCI112023 / J Clin Invest / Long-term control of plasma calcitriol concentrations in dogs and humans: Dominant role of plasma calcium concentration in experimental hyperparathyroidism. by Hulter (1985)
10.1210/jcem-48-6-1008 / J Clin Endocrinol Metab / The effects of oral CaCO3 loading and dietary calcium deprivation on plasma 1,25-dihydroxyvitamin D concentration in healthy adults. by Adams (1979)
10.1210/endo-90-3-605 / Endocrinology / The response of intestinal calcium transport to 25-hydroxy and 1,25-dihydroxyvitamin D3 in nephrectomized rats. by Boyle (1972)
10.1016/0024-3205(73)90120-3 / Life Sci / 1α,25-Dihydroxyvitamin D receptor: Competitive binding vitamin D analogs. by Brumbaugh (1973)
10.1126/science.163.3874.1450 / Science / 25-Hydroxycholecalciferol stimulation of bone resorption in tissue culture. by Trummel (1969)
10.1172/JCI106538 / J Clin Invest / Deposition in and release of vitamin D3 from body fat: Evidence for a storage site in the rat. by Rosenstreich (1971)
10.1042/cs0430413 / Clin Sci / The distribution and storage of vitamin D and its metabolites in human tissues. by Mawer (1972)
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Dates

Type	When
Created	15 years, 7 months ago (Feb. 3, 2010, 1:12 p.m.)
Deposited	1 year, 5 months ago (March 13, 2024, 10:49 a.m.)
Indexed	1 day, 13 hours ago (Sept. 4, 2025, 10:32 a.m.)
Issued	36 years, 11 months ago (Oct. 1, 1988)
Published	36 years, 11 months ago (Oct. 1, 1988)
Published Online	36 years, 11 months ago (Oct. 1, 1988)
Published Print	36 years, 11 months ago (Oct. 1, 1988)

Funders 2

Veterans Administration
Awards1
1. MO1 RR 01070
(General Clinical Research Center)
Awards1
1. AM 36066

BibTeX

@article{Dr_Bell_1988, title={Evidence of a probable role for 25-hydroxyvitamin D in the regulation of human calcium metabolism}, volume={3}, ISSN={1523-4681}, url={http://dx.doi.org/10.1002/jbmr.5650030503}, DOI={10.1002/jbmr.5650030503}, number={5}, journal={Journal of Bone and Mineral Research}, publisher={Oxford University Press (OUP)}, author={Dr. Bell, Norman H. and Epstein, Sol and Shary, Judith and Greene, Virginia and Oexmann, Mary Joan and Shaw, Sheryl}, year={1988}, month=oct, pages={489–495} }

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