DOI: 10.1002/jbmr.5650020411. A comparative immunocytochemical study on the subcellular distributions of 44 kDa bone phosphoprotein and bone γ-carboxyglutamic acid (Gla)-containing protein in osteoblasts

A comparative immunocytochemical study on the subcellular distributions of 44 kDa bone phosphoprotein and bone γ-carboxyglutamic acid (Gla)-containing protein in osteoblasts

10.1002/jbmr.5650020411

Crossref journal-article

Oxford University Press (OUP)

Journal of Bone and Mineral Research (286)

Abstract

Abstract Bone γ-carboxyglutamic acid (Gla)-containing protein (BGP or osteocalcin) and 44 kDa bone phosphoprotein (44K BPP, also called Sialoprotein I or osteopontin) have been localized at the ultrastructural level in osteoblasts from woven bones of newborn rats. Frozen, undecalcified sections of periodate-lysine-paraformal-dehyde fixed specimens were incubated with affinity purified, monospecific antibodies against BGP or 44K BPP. The sites of the antigen-antibody reaction were demonstrated by the avidin-biotin-peroxidase complex method using the Hanker-Yates reagent as a peroxidase substrate. In some cases immunostaining could only be achieved after detergent treatment. The immunostained sections were then flat-embedded in Epon 812 and processed for electron microscopy. Strong specific intracellular labeling was obtained with both antibodies, but the patterns of staining differed significantly: BGP antigenicity was mainly located in the endoplasmic reticulum (ER), whereas 44K BPP behaved as a Golgi-specific antigen. In both cases, however, we found no evidence for immunostained secretory vesicles. There was no correlation between the expression of BGP by osteoblasts and the morphological aspect of these cells, their apparent degree of polarization with respect to the bone matrix, or their relation with the mineralized phase.

Bibliography

Mark, M. P., Prince, C. W., Gay, S., Austin, R. L., Bhown, M., Finkelman, R. D., & Dr. Butler, W. T. (1987). A comparative immunocytochemical study on the subcellular distributions of 44 kDa bone phosphoprotein and bone Î³-carboxyglutamic acid (Gla)-containing protein in osteoblasts. Journal of Bone and Mineral Research, 2(4), 337â346.

Authors 7

Manuel P. Mark (first)
Charles W. Prince (additional)
Steffen Gay (additional)
Ronald L. Austin (additional)
Merra Bhown (additional)
Richard D. Finkelman (additional)
William T. Dr. Butler (additional)

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Dates

Type	When
Created	15 years, 7 months ago (Feb. 3, 2010, 1:22 p.m.)
Deposited	1 year, 5 months ago (March 13, 2024, 10:49 a.m.)
Indexed	3 months, 2 weeks ago (May 19, 2025, 8:31 a.m.)
Issued	38 years, 1 month ago (Aug. 1, 1987)
Published	38 years, 1 month ago (Aug. 1, 1987)
Published Online	38 years, 1 month ago (Aug. 1, 1987)
Published Print	38 years, 1 month ago (Aug. 1, 1987)

Funders 3

Institut de Biologie Médicale, Faculté de Médecine, Université Louis Pasteur, Strasbourg, France
National Institute of Dental Research 10.13039/100000072 National Institute of Dental and Craniofacial Research
Region: Americas
gov (National government)
Labels6
1. NIH National Institute of Dental and Craniofacial Research
2. Instituto Nacional de Investigación Dental y Craneofacial
3. El Instituto Nacional de Investigación Dental y Craneofacial
4. National Institute of Dental Research
5. NIDCR
6. NIDR
Awards2
1. DE-02670
2. DE-05092
Foundation pour la Recherche Médicale

BibTeX

@article{Mark_1987, title={A comparative immunocytochemical study on the subcellular distributions of 44 kDa bone phosphoprotein and bone γ-carboxyglutamic acid (Gla)-containing protein in osteoblasts}, volume={2}, ISSN={1523-4681}, url={http://dx.doi.org/10.1002/jbmr.5650020411}, DOI={10.1002/jbmr.5650020411}, number={4}, journal={Journal of Bone and Mineral Research}, publisher={Oxford University Press (OUP)}, author={Mark, Manuel P. and Prince, Charles W. and Gay, Steffen and Austin, Ronald L. and Bhown, Merra and Finkelman, Richard D. and Dr. Butler, William T.}, year={1987}, month=aug, pages={337–346} }

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