DOI: 10.1002/jbm.a.10021. Enhancing mechanical properties of tissue‐engineered constructs via lysyl oxidase crosslinking activity

Enhancing mechanical properties of tissue‐engineered constructs via lysyl oxidase crosslinking activity

10.1002/jbm.a.10021

Crossref journal-article

Wiley

Journal of Biomedical Materials Research Part A (311)

Abstract

AbstractA number of strategies have been investigated to enhance the mechanical stability of engineered tissues. In this study, we utilized lysyl oxidase (LO) to enzymatically crosslink extracellular matrix (ECM) proteins, particularly collagen and elastin, to enhance the mechanical integrity of the ECM and thereby impart mechanical strength to the engineered tissue. Vascular smooth muscle cells (VSMCs) were liposomally transfected with the LO gene. Both Northern and Western analyses confirmed increased LO expression. Increased LO activity was demonstrated using a fluorescent enzyme substrate assay and by observation of the presence of increased levels of desmosine, a product of LO crosslinking, in the ECM. The mechanical effects of altered crosslink densities within tissue‐engineered constructs were demonstrated in a VSMC‐populated collagen gel model. When smooth muscle cells transfected with lysyl oxidase were seeded in collagen gels, the tensile strength and elastic modulus in these constructs increased by approximately two‐fold compared to constructs seeded with mock‐transfected VSMCs. Also, desmosine levels in the LO‐populated collagen gels were higher than they were in mock‐seeded gels, as demonstrated via immunohistochemical staining. Compositional analysis of the ECM deposited by the transformed cells showed similar collagen and elastin levels, and cell proliferation rates were similar as well, thus attributing increased mechanical properties to ECM crosslinking. © 2003 Wiley Periodicals, Inc. J Biomed Mater Res 66A: 513–521, 2003

Bibliography

Elbjeirami, W. M., Yonter, E. O., Starcher, B. C., & West, J. L. (2003). Enhancing mechanical properties of tissueâengineered constructs via lysyl oxidase crosslinking activity. Journal of Biomedical Materials Research Part A, 66A(3), 513â521. Portico.

Authors 4

Wafa M. Elbjeirami (first)
Edward O. Yonter (additional)
Barry C. Starcher (additional)
Jennifer L. West (additional)

References 33 Referenced 126

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Dates

Type	When
Created	22 years ago (Aug. 11, 2003, 9:45 a.m.)
Deposited	1 year, 10 months ago (Oct. 16, 2023, 12:23 p.m.)
Indexed	3 days, 1 hour ago (Sept. 3, 2025, 6:35 a.m.)
Issued	22 years, 1 month ago (July 25, 2003)
Published	22 years, 1 month ago (July 25, 2003)
Published Online	22 years, 1 month ago (July 25, 2003)
Published Print	22 years ago (Sept. 1, 2003)

Funders 0

None

BibTeX

@article{Elbjeirami_2003, title={Enhancing mechanical properties of tissue‐engineered constructs via lysyl oxidase crosslinking activity}, volume={66A}, ISSN={1552-4965}, url={http://dx.doi.org/10.1002/jbm.a.10021}, DOI={10.1002/jbm.a.10021}, number={3}, journal={Journal of Biomedical Materials Research Part A}, publisher={Wiley}, author={Elbjeirami, Wafa M. and Yonter, Edward O. and Starcher, Barry C. and West, Jennifer L.}, year={2003}, month=jul, pages={513–521} }

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