DOI: 10.1002/jbm.820280605. Correlation between corneal epithelial cell outgrowth and monoclonal antibody binding to the cell binding domain of adsorbed fibronectin

Correlation between corneal epithelial cell outgrowth and monoclonal antibody binding to the cell binding domain of adsorbed fibronectin

10.1002/jbm.820280605

Crossref journal-article

Wiley

Journal of Biomedical Materials Research (311)

Abstract

AbstractThe ability of corneal epithelial cells to attach, spread, and migrate on synthetic surfaces is largely determined by the characteristics of the adsorbed protein layer. In previous studies we have described an in vitro model for quantitating epithelial cell outgrowth from explanted corneal buttons onto synthetic materials (Pettit et al., Invest. Ophthalmol. Vis. Sci., 31, 2269 [1990]). We have also described the role of fibronectin (fn) adsorption and binding strength on epithelial cell outgrowth (Pettit et al., J. Biomed. Mater. Res., 26, 1259 [1992]). In the current study we have used a monoclonal antibody against the RGD cell binding domain of fn (mAb 3E3) to further characterize the role of adsorbed fn in promoting epithelial cell outgrowth. Ten materials of diverse chemical and physical properties were adsorbed with fn (0.1 mg/ml) or mixtures of fn and albumin (concentrations totaling 0.1 mg/ml) and tested for antibody recognition of the cell binding domain. The surface density of bound anti‐cell binding domain antibody varied from a low of 0.66 ± 0.11 for fluorinated ethylene propylene copolymer (FEP) to a high of 1.90 ± 0.26 for tissue culture polystyrene dish substrates (units are OD at 450 nm measured in the ELISA technique normalized to polyethylene). A general increase in cell outgrowth areas was noted, with increases in recognizable cell binding domain. However, several exceptions to this trend were noted as well (e. g., low cell outgrowth but high antibody recognizability for glass). These results suggest that, although the number of cell binding domains exposed on adsorbed fn molecules may influence cell outgrowth, other characteristics of the adsorbed protein, such as the binding strength to the underlying substrate, may be equally important in characterizing epithelial cell‐substrate interactions. © 1994 John Wiley & Sons, Inc.

Bibliography

Pettit, D. K., Hoffman, A. S., & Horbett, T. A. (1994). Correlation between corneal epithelial cell outgrowth and monoclonal antibody binding to the cell binding domain of adsorbed fibronectin. Journal of Biomedical Materials Research, 28(6), 685â691. Portico.

Authors 3

Dean K. Pettit (first)
Allan S. Hoffman (additional)
Thomas A. Horbett (additional)

References 23 Referenced 48

10.1002/jbm.820150308
10.1016/S0021-9258(18)34609-X / J. Biol. Chem. / Fibronectin adsorption on hydrophilic and hydrophobic surfaces detected by antibody binding and analyzed during cell adhesion in serum‐containing medium by Grinnell F. (1982)
10.1002/jcp.1041410215
10.1002/jcp.1041090314
10.1002/jbm.820190704
10.1126/science.2821619
{'key': 'e_1_2_1_8_2', 'first-page': '2269', 'article-title': 'Quantitation of rabbit corneal epithelial cell outgrowth on polymeric substrates in vitro', 'volume': '31', 'author': 'Pettit D. K.', 'year': '1990', 'journal-title': 'Invest. Ophthalmol. Vis. Sci.'} / Invest. Ophthalmol. Vis. Sci. / Quantitation of rabbit corneal epithelial cell outgrowth on polymeric substrates in vitro by Pettit D. K. (1990)
10.1002/jbm.820261002
10.1016/0092-8674(81)90308-1
S. E.Posso “A direct enzyme‐linked immunosorbent assay (ELISA) for the measurement of fibrinogen adsorption to polymeric surfaces: A comparison between ELISA and radiolabeled fibrinogen.” Master's Thesis University of Washington Seattle Washington 1989.
10.1163/156856292X00079
10.1002/jbm.820261007
{'key': 'e_1_2_1_14_2', 'first-page': '3034', 'article-title': 'Rabbit corneal epithelial cells adhere to two distinct heparin‐binding synthetic peptides derived from fibronectin', 'volume': '33', 'author': 'Mooradian D. L.', 'year': '1992', 'journal-title': 'Invest. Ophthalmol. Vis. Sci.'} / Invest. Ophthalmol. Vis. Sci. / Rabbit corneal epithelial cells adhere to two distinct heparin‐binding synthetic peptides derived from fibronectin by Mooradian D. L. (1992)
{'key': 'e_1_2_1_15_2', 'first-page': '153', 'article-title': 'Characterization of FN‐C/H‐V, a novel synthetic peptide from fibronectin that promotes rabbit corneal epithelial cell adhesion, spreading, and motility', 'volume': '34', 'author': 'Mooradian D. L.', 'year': '1993', 'journal-title': 'Invest. Ophthalmol. Vis. Sci.'} / Invest. Ophthalmol. Vis. Sci. / Characterization of FN‐C/H‐V, a novel synthetic peptide from fibronectin that promotes rabbit corneal epithelial cell adhesion, spreading, and motility by Mooradian D. L. (1993)
10.1021/bi00159a008
10.3109/02713688809047035
{'key': 'e_1_2_1_18_2', 'first-page': '2766', 'article-title': 'Type IV collagen and corneal epithelial cell adhesion and migration', 'volume': '32', 'author': 'Cameron J. D.', 'year': '1991', 'journal-title': 'Invest. Ophthalmol. Vis. Sci.'} / Invest. Ophthalmol. Vis. Sci. / Type IV collagen and corneal epithelial cell adhesion and migration by Cameron J. D. (1991)
10.1097/00003226-199004000-00008
10.1002/jbm.820150506
{'key': 'e_1_2_1_21_2', 'first-page': '1', 'volume-title': 'Proteins at Interfaces Physiochemical and Biochemical studies, ACS Symposium Series', 'author': 'Horbett T. A.', 'year': '1987'} / Proteins at Interfaces Physiochemical and Biochemical studies, ACS Symposium Series by Horbett T. A. (1987)
10.1182/blood.V68.2.355.355 / Blood / Does the conformation of adsorbed fibrinogen dictate platelet interactions with artificial surfaces? by Lindon J. N. (1986)
{'key': 'e_1_2_1_23_2', 'article-title': 'Antibody‐detectable changes in fibrinogen adsorption affecting platelet activation on polymer surfaces', 'volume': '260', 'author': 'Shiba E.', 'journal-title': 'Am. J. Physiol.'} / Am. J. Physiol. / Antibody‐detectable changes in fibrinogen adsorption affecting platelet activation on polymer surfaces by Shiba E.
{'key': 'e_1_2_1_23_3', 'first-page': 'C965', 'volume': '29', 'year': '1991', 'journal-title': 'Cell Physiol.'} / Cell Physiol. (1991)

Dates

Type	When
Created	20 years, 3 months ago (May 27, 2005, 3:34 p.m.)
Deposited	1 year, 11 months ago (Sept. 27, 2023, 3:05 p.m.)
Indexed	2 months, 1 week ago (June 26, 2025, 8:30 a.m.)
Issued	31 years, 3 months ago (June 1, 1994)
Published	31 years, 3 months ago (June 1, 1994)
Published Online	20 years, 11 months ago (Sept. 13, 2004)
Published Print	31 years, 3 months ago (June 1, 1994)

Funders 0

None

BibTeX

@article{Pettit_1994, title={Correlation between corneal epithelial cell outgrowth and monoclonal antibody binding to the cell binding domain of adsorbed fibronectin}, volume={28}, ISSN={1097-4636}, url={http://dx.doi.org/10.1002/jbm.820280605}, DOI={10.1002/jbm.820280605}, number={6}, journal={Journal of Biomedical Materials Research}, publisher={Wiley}, author={Pettit, Dean K. and Hoffman, Allan S. and Horbett, Thomas A.}, year={1994}, month=jun, pages={685–691} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        6,
        26
      ]
    ],
    "date-time": "2025-06-26T12:30:20Z",
    "timestamp": 1750941020117
  },
  "reference-count": 23,
  "publisher": "Wiley",
  "issue": "6",
  "license": [
    {
      "start": {
        "date-parts": [
          [
            2004,
            9,
            13
          ]
        ],
        "date-time": "2004-09-13T00:00:00Z",
        "timestamp": 1095033600000
      },
      "content-version": "vor",
      "delay-in-days": 3757,
      "URL": "http://onlinelibrary.wiley.com/termsAndConditions#vor"
    }
  ],
  "content-domain": {
    "domain": [],
    "crossmark-restriction": false
  },
  "published-print": {
    "date-parts": [
      [
        1994,
        6
      ]
    ]
  },
  "abstract": "<jats:title>Abstract</jats:title><jats:p>The ability of corneal epithelial cells to attach, spread, and migrate on synthetic surfaces is largely determined by the characteristics of the adsorbed protein layer. In previous studies we have described an <jats:italic>in vitro</jats:italic> model for quantitating epithelial cell outgrowth from explanted corneal buttons onto synthetic materials (Pettit <jats:italic>et al., Invest. Ophthalmol. Vis. Sci.</jats:italic>, <jats:bold>31</jats:bold>, 2269 [1990]). We have also described the role of fibronectin (fn) adsorption and binding strength on epithelial cell outgrowth (Pettit <jats:italic>et al., J. Biomed. Mater. Res.</jats:italic>, <jats:bold>26</jats:bold>, 1259 [1992]). In the current study we have used a monoclonal antibody against the RGD cell binding domain of fn (mAb 3E3) to further characterize the role of adsorbed fn in promoting epithelial cell outgrowth. Ten materials of diverse chemical and physical properties were adsorbed with fn (0.1 mg/ml) or mixtures of fn and albumin (concentrations totaling 0.1 mg/ml) and tested for antibody recognition of the cell binding domain. The surface density of bound anti\u2010cell binding domain antibody varied from a low of 0.66 \u00b1 0.11 for fluorinated ethylene propylene copolymer (FEP) to a high of 1.90 \u00b1 0.26 for tissue culture polystyrene dish substrates (units are OD at 450 nm measured in the ELISA technique normalized to polyethylene). A general increase in cell outgrowth areas was noted, with increases in recognizable cell binding domain. However, several exceptions to this trend were noted as well (e. g., low cell outgrowth but high antibody recognizability for glass). These results suggest that, although the number of cell binding domains exposed on adsorbed fn molecules may influence cell outgrowth, other characteristics of the adsorbed protein, such as the binding strength to the underlying substrate, may be equally important in characterizing epithelial cell\u2010substrate interactions. \u00a9 1994 John Wiley &amp; Sons, Inc.</jats:p>",
  "DOI": "10.1002/jbm.820280605",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2005,
        5,
        27
      ]
    ],
    "date-time": "2005-05-27T19:34:41Z",
    "timestamp": 1117222481000
  },
  "page": "685-691",
  "source": "Crossref",
  "is-referenced-by-count": 48,
  "title": "Correlation between corneal epithelial cell outgrowth and monoclonal antibody binding to the cell binding domain of adsorbed fibronectin",
  "prefix": "10.1002",
  "volume": "28",
  "author": [
    {
      "given": "Dean K.",
      "family": "Pettit",
      "sequence": "first",
      "affiliation": []
    },
    {
      "given": "Allan S.",
      "family": "Hoffman",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Thomas A.",
      "family": "Horbett",
      "sequence": "additional",
      "affiliation": []
    }
  ],
  "member": "311",
  "published-online": {
    "date-parts": [
      [
        2004,
        9,
        13
      ]
    ]
  },
  "reference": [
    {
      "key": "e_1_2_1_2_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/jbm.820150308"
    },
    {
      "key": "e_1_2_1_3_2",
      "doi-asserted-by": "crossref",
      "first-page": "4888",
      "DOI": "10.1016/S0021-9258(18)34609-X",
      "article-title": "Fibronectin adsorption on hydrophilic and hydrophobic surfaces detected by antibody binding and analyzed during cell adhesion in serum\u2010containing medium",
      "volume": "257",
      "author": "Grinnell F.",
      "year": "1982",
      "journal-title": "J. Biol. Chem."
    },
    {
      "key": "e_1_2_1_4_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/jcp.1041410215"
    },
    {
      "key": "e_1_2_1_5_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/jcp.1041090314"
    },
    {
      "key": "e_1_2_1_6_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/jbm.820190704"
    },
    {
      "key": "e_1_2_1_7_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.2821619"
    },
    {
      "key": "e_1_2_1_8_2",
      "first-page": "2269",
      "article-title": "Quantitation of rabbit corneal epithelial cell outgrowth on polymeric substrates in vitro",
      "volume": "31",
      "author": "Pettit D. K.",
      "year": "1990",
      "journal-title": "Invest. Ophthalmol. Vis. Sci."
    },
    {
      "key": "e_1_2_1_9_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/jbm.820261002"
    },
    {
      "key": "e_1_2_1_10_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(81)90308-1"
    },
    {
      "key": "e_1_2_1_11_2",
      "unstructured": "S. E.Posso \u201cA direct enzyme\u2010linked immunosorbent assay (ELISA) for the measurement of fibrinogen adsorption to polymeric surfaces: A comparison between ELISA and radiolabeled fibrinogen.\u201d Master's Thesis University of Washington Seattle Washington 1989."
    },
    {
      "key": "e_1_2_1_12_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1163/156856292X00079"
    },
    {
      "key": "e_1_2_1_13_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/jbm.820261007"
    },
    {
      "key": "e_1_2_1_14_2",
      "first-page": "3034",
      "article-title": "Rabbit corneal epithelial cells adhere to two distinct heparin\u2010binding synthetic peptides derived from fibronectin",
      "volume": "33",
      "author": "Mooradian D. L.",
      "year": "1992",
      "journal-title": "Invest. Ophthalmol. Vis. Sci."
    },
    {
      "key": "e_1_2_1_15_2",
      "first-page": "153",
      "article-title": "Characterization of FN\u2010C/H\u2010V, a novel synthetic peptide from fibronectin that promotes rabbit corneal epithelial cell adhesion, spreading, and motility",
      "volume": "34",
      "author": "Mooradian D. L.",
      "year": "1993",
      "journal-title": "Invest. Ophthalmol. Vis. Sci."
    },
    {
      "key": "e_1_2_1_16_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1021/bi00159a008"
    },
    {
      "key": "e_1_2_1_17_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.3109/02713688809047035"
    },
    {
      "key": "e_1_2_1_18_2",
      "first-page": "2766",
      "article-title": "Type IV collagen and corneal epithelial cell adhesion and migration",
      "volume": "32",
      "author": "Cameron J. D.",
      "year": "1991",
      "journal-title": "Invest. Ophthalmol. Vis. Sci."
    },
    {
      "key": "e_1_2_1_19_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1097/00003226-199004000-00008"
    },
    {
      "key": "e_1_2_1_20_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/jbm.820150506"
    },
    {
      "key": "e_1_2_1_21_2",
      "first-page": "1",
      "volume-title": "Proteins at Interfaces Physiochemical and Biochemical studies, ACS Symposium Series",
      "author": "Horbett T. A.",
      "year": "1987"
    },
    {
      "key": "e_1_2_1_22_2",
      "doi-asserted-by": "crossref",
      "first-page": "355",
      "DOI": "10.1182/blood.V68.2.355.355",
      "article-title": "Does the conformation of adsorbed fibrinogen dictate platelet interactions with artificial surfaces?",
      "volume": "68",
      "author": "Lindon J. N.",
      "year": "1986",
      "journal-title": "Blood"
    },
    {
      "key": "e_1_2_1_23_2",
      "article-title": "Antibody\u2010detectable changes in fibrinogen adsorption affecting platelet activation on polymer surfaces",
      "volume": "260",
      "author": "Shiba E.",
      "journal-title": "Am. J. Physiol."
    },
    {
      "key": "e_1_2_1_23_3",
      "first-page": "C965",
      "volume": "29",
      "year": "1991",
      "journal-title": "Cell Physiol."
    }
  ],
  "container-title": "Journal of Biomedical Materials Research",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fjbm.820280605",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "text-mining"
    },
    {
      "URL": "https://onlinelibrary.wiley.com/doi/pdf/10.1002/jbm.820280605",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2023,
        9,
        27
      ]
    ],
    "date-time": "2023-09-27T19:05:00Z",
    "timestamp": 1695841500000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://onlinelibrary.wiley.com/doi/10.1002/jbm.820280605"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1994,
        6
      ]
    ]
  },
  "references-count": 23,
  "journal-issue": {
    "issue": "6",
    "published-print": {
      "date-parts": [
        [
          1994,
          6
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1002/jbm.820280605"
  ],
  "URL": "http://dx.doi.org/10.1002/jbm.820280605",
  "relation": {},
  "ISSN": [
    "0021-9304",
    "1097-4636"
  ],
  "subject": [],
  "container-title-short": "J. Biomed. Mater. Res.",
  "published": {
    "date-parts": [
      [
        1994,
        6
      ]
    ]
  }
}