DOI: 10.1002/jbm.820120106. Protein sorption on polymer surfaces measured by fluorescence labels

Protein sorption on polymer surfaces measured by fluorescence labels

10.1002/jbm.820120106

Crossref journal-article

Wiley

Journal of Biomedical Materials Research (311)

Abstract

AbstractFluorescence labeling can be used in studying protein sorption on various surfaces with a sensitivity of about 10−8 g/cm2, commensurate with radioactive labeling. Fluorescamine proved to be the most suitable compound for studying protein sorption on hydrophilic gels, because, unlike fluoresceine isothiocyanate and dansylchloride, free fluorochrome does not interfere with measurements. Sorption properties of labeled serum albumin were tested on poly(2‐hydroxyethyl methacrylate), on the copolymer of 2‐hydroxyethyl methacrylate with methyl methacrylate, and on polyethylene. Labeling does not cause aggregation of the protein, but, as expected, it shifts and somewhat broadens its electrophoretic band while at the same time slightly raising its affinity toward hydrophobic surfaces.

Bibliography

Brynda, E., DrobnÃk, J., VacÃk, J., & KÃ¡lal, J. (1978). Protein sorption on polymer surfaces measured by fluorescence labels. Journal of Biomedical Materials Research, 12(1), 55â65. Portico.

Authors 4

E. Brynda (first)
J. Drobník (additional)
J. Vacík (additional)
J. Kálal (additional)

References 14 Referenced 19

10.1002/jbm.820030115
{'key': 'e_1_2_1_3_2', 'first-page': '225', 'volume': '48', 'author': 'Salzman E. W.', 'year': '1972', 'journal-title': 'Bull. N. Y. Acad. Med.'} / Bull. N. Y. Acad. Med. by Salzman E. W. (1972)
10.1021/ba-1975-0145.ch012
{'key': 'e_1_2_1_5_2', 'first-page': '686', 'volume': '73', 'author': 'Packham M. A.', 'year': '1969', 'journal-title': 'J. Lab. Clin. Med.'} / J. Lab. Clin. Med. by Packham M. A. (1969)
{'key': 'e_1_2_1_6_2', 'first-page': '280', 'volume': '81', 'author': 'Jenkins C. S. P.', 'year': '1973', 'journal-title': 'J. Lab. Clin. Med.'} / J. Lab. Clin. Med. by Jenkins C. S. P. (1973)
{'key': 'e_1_2_1_7_2', 'first-page': '109', 'volume': '42', 'author': 'Lyman D. J.', 'year': '1971', 'journal-title': 'Thromb. Hemorrh. Suppl.'} / Thromb. Hemorrh. Suppl. by Lyman D. J. (1971)
10.1097/00002480-197301900-00001
{'key': 'e_1_2_1_9_2', 'volume-title': 'Fluorescent Protein Tracing', 'author': 'Nairn R. C.', 'year': '1964'} / Fluorescent Protein Tracing by Nairn R. C. (1964)
T. P.Murtha Disertation University Microfilms Ann Arbor Mich. 69–9879(1968).
10.1021/ja00771a084
10.1016/S0003-9861(73)80023-2
10.1042/bj0510155
{'key': 'e_1_2_1_14_2', 'first-page': '3877', 'volume-title': 'Macromolecular Chemistry, Prague 1965 (J. Polym. Sci. C, 16)', 'author': 'Kopeček J.', 'year': '1968'} / Macromolecular Chemistry, Prague 1965 (J. Polym. Sci. C, 16) by Kopeček J. (1968)
10.1021/ac60326a001

Dates

Type	When
Created	20 years, 7 months ago (Dec. 31, 2004, 7:05 a.m.)
Deposited	1 year, 9 months ago (Nov. 12, 2023, 5:18 a.m.)
Indexed	1 year, 9 months ago (Nov. 12, 2023, 5:40 a.m.)
Issued	47 years, 7 months ago (Jan. 1, 1978)
Published	47 years, 7 months ago (Jan. 1, 1978)
Published Online	20 years, 11 months ago (Sept. 13, 2004)
Published Print	47 years, 7 months ago (Jan. 1, 1978)

Funders 0

None

BibTeX

@article{Brynda_1978, title={Protein sorption on polymer surfaces measured by fluorescence labels}, volume={12}, ISSN={1097-4636}, url={http://dx.doi.org/10.1002/jbm.820120106}, DOI={10.1002/jbm.820120106}, number={1}, journal={Journal of Biomedical Materials Research}, publisher={Wiley}, author={Brynda, E. and Drobník, J. and Vacík, J. and Kálal, J.}, year={1978}, month=jan, pages={55–65} }

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