DOI: 10.1002/jbm.10110. Viability, adhesion, and bone phenotype of osteoblast‐like cells on polyelectrolyte multilayer films

Viability, adhesion, and bone phenotype of osteoblast‐like cells on polyelectrolyte multilayer films

10.1002/jbm.10110

Crossref journal-article

Wiley

Journal of Biomedical Materials Research (311)

Abstract

AbstractThe aim of this study was to develop new biocompatible coatings for bone implants by the alternating deposition of oppositely charged polyelectrolytes. Polyelectrolyte films were built up with different terminating layers on which SaOS‐2 osteoblast‐like cells and human periodontal ligament (PDL) cells were grown. The terminating layer was made of one of the following polyelectrolytes: poly(ethylene imine) (PEI), poly(sodium 4‐styrenesulfonate) (PSS), poly(allylamine hydrochloride) (PAH), poly(L‐glutamic acid) (PGA), or poly(L‐lysine) (PLL). Cell adherence, viability, stability of osteoblast phenotype, and inflammatory response were studied. Adherence and viability were good on all terminating layers except the PEI‐terminating layer, which was cytotoxic. Maintenance of osteoblast phenotype marker expression was observed on PSS‐ and PGA‐terminating films for both cell types, whereas downregulation, associated with the induction of Interleukin‐8 (IL‐8) secretion, was detected on PEI and PAH for both cell types and on PLL for PDL cells. These results suggested a good biocompatibility of PSS‐ and PGA‐ending films for PDL cells and of PSS‐, PGA‐, and PLL‐terminating films for SaOS‐2 cells. As a result, polyelectrolyte multilayer films could emerge as new alternatives for implant coatings. © 2002 Wiley Periodicals, Inc. J Biomed Mater Res 60: 657–667, 2002

Bibliography

TryoenâTÃ³th, P., Vautier, D., Haikel, Y., Voegel, J., Schaaf, P., Chluba, J., & Ogier, J. (2002). Viability, adhesion, and bone phenotype of osteoblastâlike cells on polyelectrolyte multilayer films. Journal of Biomedical Materials Research, 60(4), 657â667. Portico.

Authors 7

Petra Tryoen‐Tóth (first)
Dominique Vautier (additional)
Youssef Haikel (additional)
Jean‐Claude Voegel (additional)
Pierre Schaaf (additional)
Johanna Chluba (additional)
Joëlle Ogier (additional)

References 46 Referenced 184

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Dates

Type	When
Created	23 years ago (Aug. 25, 2002, 7:44 p.m.)
Deposited	1 year, 10 months ago (Oct. 16, 2023, 9:26 a.m.)
Indexed	1 week, 2 days ago (Aug. 21, 2025, 1:02 p.m.)
Issued	23 years, 5 months ago (March 29, 2002)
Published	23 years, 5 months ago (March 29, 2002)
Published Online	23 years, 5 months ago (March 29, 2002)
Published Print	23 years, 2 months ago (June 15, 2002)

Funders 4

INSERM-CNRS Ingéniérie Tissulaire
Institut Francais pour la Recherche Odontologique
Association pour la Recherche sur le Cancer 10.13039/100007391
Region: Europe
pri (Associations and societies (private and public))
Labels1
1. ARC
Ligue Nationale Contre le Cancer (Comité du Bas-Rhin).

BibTeX

@article{Tryoen_T_th_2002, title={Viability, adhesion, and bone phenotype of osteoblast‐like cells on polyelectrolyte multilayer films}, volume={60}, ISSN={1097-4636}, url={http://dx.doi.org/10.1002/jbm.10110}, DOI={10.1002/jbm.10110}, number={4}, journal={Journal of Biomedical Materials Research}, publisher={Wiley}, author={Tryoen‐Tóth, Petra and Vautier, Dominique and Haikel, Youssef and Voegel, Jean‐Claude and Schaaf, Pierre and Chluba, Johanna and Ogier, Joëlle}, year={2002}, month=mar, pages={657–667} }

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