DOI: 10.1002/jbio.200910062. Scoring of collagen organization in healthy and diseased human dermis by multiphoton microscopy

Scoring of collagen organization in healthy and diseased human dermis by multiphoton microscopy

10.1002/jbio.200910062

Crossref journal-article

Wiley

Journal of Biophotonics (311)

Abstract

AbstractWe have used nonlinear imaging to evaluate collagen organization in connective tissue ex‐vivo samples. Image analysis methods were tested on healthy dermis, normal scars, and keloids. The evaluation of the second harmonic to autofluorescence aging index of dermis (SAAID) has allowed a first characterization of tissues by scoring the collagen/elastin content. Further analyses on collagen morphology in healthy dermis and keloids were performed by image‐pattern analysis of SHG images. The gray‐level co‐occurrence matrix (GLCM) analysis method has allowed classification of different tissues based on the evaluation of geometrical arrangement of collagen fibrillar bundles, whereas a pattern analysis of the FFT images has allowed the discrimination of different tissues based on the anisotropy of collagen fibers distribution. This multiple scoring method represents a promising tool to be extended to other collagen disorders, as well as to be used in in‐vivo skin‐imaging applications. (© 2010 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)

Bibliography

Cicchi, R., Kapsokalyvas, D., De Giorgi, V., Maio, V., Van Wiechen, A., Massi, D., Lotti, T., & Pavone, F. S. (2009). Scoring of collagen organization in healthy and diseased human dermis by multiphoton microscopy. Journal of Biophotonics, 3(1â2), 34â43. Portico.

Authors 8

Riccardo Cicchi (first)
Dimitrios Kapsokalyvas (additional)
Vincenzo De Giorgi (additional)
Vincenza Maio (additional)
Annelies Van Wiechen (additional)
Daniela Massi (additional)
Torello Lotti (additional)
Francesco S. Pavone (additional)

References 40 Referenced 183

10.1126/science.2321027
10.1038/nbt899
10.1073/pnas.172368799
10.1073/pnas.0832308100
10.1007/s101030050074
10.1364/OE.3.000339
10.1034/j.1600-0560.2002.290802.x
10.1364/OE.8.000002
10.1117/1.1577349
10.1111/j.1600-0625.2007.00669.x
10.1111/j.1600-0625.2008.00815.x
10.1038/jid.2008.439
10.1016/S0006-3495(01)76129-2
10.1038/nbt894
10.1016/S0006-3495(02)75414-3
10.1063/1.437677
10.1016/j.biomaterials.2004.02.059
10.1529/biophysj.104.047308
{'key': 'e_1_2_1_20_2', 'first-page': '4054', 'volume': '15', 'author': 'Strupler M.', 'year': '2007', 'journal-title': 'Second harmonic imaging and scoring of collagen in fibrotic tissues, Opt. Exp.'} / Second harmonic imaging and scoring of collagen in fibrotic tissues, Opt. Exp. by Strupler M. (2007)
10.1016/S0006-3495(02)75673-7
10.1117/1.1644116
10.1529/biophysj.106.085902
10.1364/OE.17.011161
10.1001/archfacial.2007.18
10.1364/OPEX.13.005791
10.1364/OE.17.004868
10.1038/nm879
10.1364/OL.31.002756
10.1186/1741-7015-4-38
10.1002/jbio.200710003
10.1364/OL.30.002275
10.1364/OL.31.002879
10.1364/OE.15.010135
10.1038/sj.jid.5701139
H.Lodish A.Berk S. L.Zipursky P.Matsudaira D.BaltimoreandJ.Darnell Molecular Cell Biology 4th edn. (W. H. Freeman & Co. New York 2000) pp. 979–985.
10.1073/pnas.0603216103
10.1561/0600000013
10.1529/biophysj.107.111047
10.1046/j.1524-4725.1999.98257.x
M.Hall‐Beyer The GLCM Tutorial Homepage 2.10 (2007)http://www.fp.ucalgary.ca/mhallbey/tutorial.htm.

Dates

Type	When
Created	15 years, 11 months ago (Sept. 21, 2009, 8:45 a.m.)
Deposited	1 year, 11 months ago (Sept. 1, 2023, 2:03 p.m.)
Indexed	4 weeks ago (Aug. 2, 2025, 1:13 a.m.)
Issued	15 years, 8 months ago (Dec. 22, 2009)
Published	15 years, 8 months ago (Dec. 22, 2009)
Published Online	15 years, 8 months ago (Dec. 22, 2009)
Published Print	15 years, 7 months ago (Jan. 1, 2010)

Funders 0

None

BibTeX

@article{Cicchi_2009, title={Scoring of collagen organization in healthy and diseased human dermis by multiphoton microscopy}, volume={3}, ISSN={1864-0648}, url={http://dx.doi.org/10.1002/jbio.200910062}, DOI={10.1002/jbio.200910062}, number={1–2}, journal={Journal of Biophotonics}, publisher={Wiley}, author={Cicchi, Riccardo and Kapsokalyvas, Dimitrios and De Giorgi, Vincenzo and Maio, Vincenza and Van Wiechen, Annelies and Massi, Daniela and Lotti, Torello and Pavone, Francesco S.}, year={2009}, month=dec, pages={34–43} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        8,
        2
      ]
    ],
    "date-time": "2025-08-02T05:13:09Z",
    "timestamp": 1754111589656
  },
  "reference-count": 40,
  "publisher": "Wiley",
  "issue": "1-2",
  "license": [
    {
      "start": {
        "date-parts": [
          [
            2009,
            9,
            21
          ]
        ],
        "date-time": "2009-09-21T00:00:00Z",
        "timestamp": 1253491200000
      },
      "content-version": "vor",
      "delay-in-days": 0,
      "URL": "http://onlinelibrary.wiley.com/termsAndConditions#vor"
    }
  ],
  "content-domain": {
    "domain": [],
    "crossmark-restriction": false
  },
  "published-print": {
    "date-parts": [
      [
        2010,
        1
      ]
    ]
  },
  "abstract": "<jats:title>Abstract</jats:title><jats:p>We have used nonlinear imaging to evaluate collagen organization in connective tissue <jats:italic>ex\u2010vivo</jats:italic> samples. Image analysis methods were tested on healthy dermis, normal scars, and keloids. The evaluation of the second harmonic to autofluorescence aging index of dermis (SAAID) has allowed a first characterization of tissues by scoring the collagen/elastin content. Further analyses on collagen morphology in healthy dermis and keloids were performed by image\u2010pattern analysis of SHG images. The gray\u2010level co\u2010occurrence matrix (GLCM) analysis method has allowed classification of different tissues based on the evaluation of geometrical arrangement of collagen fibrillar bundles, whereas a pattern analysis of the FFT images has allowed the discrimination of different tissues based on the anisotropy of collagen fibers distribution. This multiple scoring method represents a promising tool to be extended to other collagen disorders, as well as to be used in <jats:italic>in\u2010vivo</jats:italic> skin\u2010imaging applications. (\u00a9 2010 WILEY\u2010VCH Verlag GmbH &amp; Co. KGaA, Weinheim)</jats:p>",
  "DOI": "10.1002/jbio.200910062",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2009,
        9,
        21
      ]
    ],
    "date-time": "2009-09-21T12:45:01Z",
    "timestamp": 1253537101000
  },
  "page": "34-43",
  "source": "Crossref",
  "is-referenced-by-count": 183,
  "title": "Scoring of collagen organization in healthy and diseased human dermis by multiphoton microscopy",
  "prefix": "10.1002",
  "volume": "3",
  "author": [
    {
      "given": "Riccardo",
      "family": "Cicchi",
      "sequence": "first",
      "affiliation": []
    },
    {
      "given": "Dimitrios",
      "family": "Kapsokalyvas",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Vincenzo",
      "family": "De Giorgi",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Vincenza",
      "family": "Maio",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Annelies",
      "family": "Van Wiechen",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Daniela",
      "family": "Massi",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Torello",
      "family": "Lotti",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Francesco S.",
      "family": "Pavone",
      "sequence": "additional",
      "affiliation": []
    }
  ],
  "member": "311",
  "published-online": {
    "date-parts": [
      [
        2009,
        12,
        22
      ]
    ]
  },
  "reference": [
    {
      "key": "e_1_2_1_2_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.2321027"
    },
    {
      "key": "e_1_2_1_3_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/nbt899"
    },
    {
      "key": "e_1_2_1_4_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.172368799"
    },
    {
      "key": "e_1_2_1_5_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.0832308100"
    },
    {
      "key": "e_1_2_1_6_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1007/s101030050074"
    },
    {
      "key": "e_1_2_1_7_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1364/OE.3.000339"
    },
    {
      "key": "e_1_2_1_8_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1034/j.1600-0560.2002.290802.x"
    },
    {
      "key": "e_1_2_1_9_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1364/OE.8.000002"
    },
    {
      "key": "e_1_2_1_10_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1117/1.1577349"
    },
    {
      "key": "e_1_2_1_11_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1600-0625.2007.00669.x"
    },
    {
      "key": "e_1_2_1_12_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1600-0625.2008.00815.x"
    },
    {
      "key": "e_1_2_1_13_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/jid.2008.439"
    },
    {
      "key": "e_1_2_1_14_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0006-3495(01)76129-2"
    },
    {
      "key": "e_1_2_1_15_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/nbt894"
    },
    {
      "key": "e_1_2_1_16_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0006-3495(02)75414-3"
    },
    {
      "key": "e_1_2_1_17_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1063/1.437677"
    },
    {
      "key": "e_1_2_1_18_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/j.biomaterials.2004.02.059"
    },
    {
      "key": "e_1_2_1_19_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1529/biophysj.104.047308"
    },
    {
      "key": "e_1_2_1_20_2",
      "first-page": "4054",
      "volume": "15",
      "author": "Strupler M.",
      "year": "2007",
      "journal-title": "Second harmonic imaging and scoring of collagen in fibrotic tissues, Opt. Exp."
    },
    {
      "key": "e_1_2_1_21_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0006-3495(02)75673-7"
    },
    {
      "key": "e_1_2_1_22_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1117/1.1644116"
    },
    {
      "key": "e_1_2_1_23_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1529/biophysj.106.085902"
    },
    {
      "key": "e_1_2_1_24_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1364/OE.17.011161"
    },
    {
      "key": "e_1_2_1_25_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1001/archfacial.2007.18"
    },
    {
      "key": "e_1_2_1_26_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1364/OPEX.13.005791"
    },
    {
      "key": "e_1_2_1_27_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1364/OE.17.004868"
    },
    {
      "key": "e_1_2_1_28_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/nm879"
    },
    {
      "key": "e_1_2_1_29_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1364/OL.31.002756"
    },
    {
      "key": "e_1_2_1_30_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1186/1741-7015-4-38"
    },
    {
      "key": "e_1_2_1_31_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/jbio.200710003"
    },
    {
      "key": "e_1_2_1_32_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1364/OL.30.002275"
    },
    {
      "key": "e_1_2_1_33_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1364/OL.31.002879"
    },
    {
      "key": "e_1_2_1_34_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1364/OE.15.010135"
    },
    {
      "key": "e_1_2_1_35_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/sj.jid.5701139"
    },
    {
      "key": "e_1_2_1_36_2",
      "unstructured": "H.Lodish A.Berk S. L.Zipursky P.Matsudaira D.BaltimoreandJ.Darnell Molecular Cell Biology 4th edn. (W. H. Freeman & Co. New York 2000) pp. 979\u2013985."
    },
    {
      "key": "e_1_2_1_37_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.0603216103"
    },
    {
      "key": "e_1_2_1_38_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1561/0600000013"
    },
    {
      "key": "e_1_2_1_39_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1529/biophysj.107.111047"
    },
    {
      "key": "e_1_2_1_40_2",
      "doi-asserted-by": "publisher",
      "DOI": "10.1046/j.1524-4725.1999.98257.x"
    },
    {
      "key": "e_1_2_1_41_2",
      "unstructured": "M.Hall\u2010Beyer The GLCM Tutorial Homepage 2.10 (2007)http://www.fp.ucalgary.ca/mhallbey/tutorial.htm."
    }
  ],
  "container-title": "Journal of Biophotonics",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fjbio.200910062",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "text-mining"
    },
    {
      "URL": "https://onlinelibrary.wiley.com/doi/pdf/10.1002/jbio.200910062",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2023,
        9,
        1
      ]
    ],
    "date-time": "2023-09-01T18:03:09Z",
    "timestamp": 1693591389000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://onlinelibrary.wiley.com/doi/10.1002/jbio.200910062"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        2009,
        12,
        22
      ]
    ]
  },
  "references-count": 40,
  "journal-issue": {
    "issue": "1-2",
    "published-print": {
      "date-parts": [
        [
          2010,
          1
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1002/jbio.200910062"
  ],
  "URL": "http://dx.doi.org/10.1002/jbio.200910062",
  "relation": {},
  "ISSN": [
    "1864-063X",
    "1864-0648"
  ],
  "subject": [],
  "container-title-short": "Journal of Biophotonics",
  "published": {
    "date-parts": [
      [
        2009,
        12,
        22
      ]
    ]
  }
}