DOI: 10.1002/immu.200310022. Biochemical and functional characterization of the interaction between pentraxin 3 and C1q

Biochemical and functional characterization of the interaction between pentraxin 3 and C1q

10.1002/immu.200310022

Crossref journal-article

Wiley

European Journal of Immunology (311)

Abstract

AbstractPentraxin 3 (PTX3) is a recently characterized member of the pentraxin family of acute‐phase proteins produced during inflammation. Classical short pentraxins, C‐reactive protein, and serum amyloid P component can bind to C1q and thereby activate the classical complement pathway. Since PTX3 can also bind C1q, the present study was designed to define the interaction between PTX3 and C1q and to examine the functional consequences of this interaction. A dose‐dependent binding of both C1q and the C1 complex to PTX3 was observed. Experiments with recombinant globular head domains of human C1q A, B, and C chains indicated that C1q interacts with PTX3 via its globular head region. Binding of C1q to immobilized PTX3 induced activation of the classical complement pathway as assessed by C4 deposition. Furthermore, PTX3 enhanced C1q binding and complement activation on apoptotic cells. However, in the fluid‐phase, pre‐incubation of PTX3 with C1q resulted in inhibition of complement activation by blocking the interaction of C1q with immunoglobulins. These results indicate that PTX3 can both inhibit and activate the classical complement pathway by binding C1q, depending on the way it is presented. PTX3 may therefore be involved in the regulation of the innate immune response.

Bibliography

Nauta, A. J., Bottazzi, B., Mantovani, A., Salvatori, G., Kishore, U., Schwaeble, W. J., Gingras, A. R., Tzima, S., Vivanco, F., Egido, J., Tijsma, O., Hack, E. C., Daha, M. R., & Roos, A. (2003). Biochemical and functional characterization of the interaction between pentraxin 3 and C1q. European Journal of Immunology, 33(2), 465â473. Portico.

Authors 14

Alma J. Nauta (first)
Barbara Bottazzi (additional)
Alberto Mantovani (additional)
Giovanni Salvatori (additional)
Uday Kishore (additional)
Wilhelm J. Schwaeble (additional)
Alexandre R. Gingras (additional)
Sotiria Tzima (additional)
Fernando Vivanco (additional)
Jesús Egido (additional)
Odette Tijsma (additional)
Erik C. Hack (additional)
Mohamed R. Daha (additional)
Anja Roos (additional)

References 37 Referenced 291

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{'key': 'e_1_2_7_24_2', 'article-title': 'Modular organization of the carboxy‐terminal, globular head regions of human C1q A, B, and C chains.', 'author': 'Kishore U.', 'year': '2002', 'journal-title': 'J. Immunol.'} / J. Immunol. / Modular organization of the carboxy‐terminal, globular head regions of human C1q A, B, and C chains. by Kishore U. (2002)
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Dates

Type	When
Created	22 years, 6 months ago (Feb. 5, 2003, 8:43 p.m.)
Deposited	1 year, 9 months ago (Nov. 17, 2023, 7:15 p.m.)
Indexed	1 day, 14 hours ago (Sept. 3, 2025, 6:22 a.m.)
Issued	22 years, 7 months ago (Jan. 27, 2003)
Published	22 years, 7 months ago (Jan. 27, 2003)
Published Online	22 years, 7 months ago (Jan. 27, 2003)
Published Print	22 years, 7 months ago (Feb. 1, 2003)

Funders 0

None

BibTeX

@article{Nauta_2003, title={Biochemical and functional characterization of the interaction between pentraxin 3 and C1q}, volume={33}, ISSN={1521-4141}, url={http://dx.doi.org/10.1002/immu.200310022}, DOI={10.1002/immu.200310022}, number={2}, journal={European Journal of Immunology}, publisher={Wiley}, author={Nauta, Alma J. and Bottazzi, Barbara and Mantovani, Alberto and Salvatori, Giovanni and Kishore, Uday and Schwaeble, Wilhelm J. and Gingras, Alexandre R. and Tzima, Sotiria and Vivanco, Fernando and Egido, Jesús and Tijsma, Odette and Hack, Erik C. and Daha, Mohamed R. and Roos, Anja}, year={2003}, month=jan, pages={465–473} }

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