DOI: 10.1002/ijc.2910630422. Oncogenic transformation alters cisplatin‐induced apoptosis in rat embryo fibroblasts

Oncogenic transformation alters cisplatin‐induced apoptosis in rat embryo fibroblasts

10.1002/ijc.2910630422

Crossref journal-article

Wiley

International Journal of Cancer (311)

Abstract

AbstractWe have investigated whether cellular transformation by different oncogenes alters their responsiveness to the chemotherapeutic agent cis‐diamminedichloroplatinum(lI) (cDDP). Rat‐6 (R6) embryo fibroblasts transformed by v‐myc, rc‐mos (rearranged mouse c‐mos), v‐src and activated c‐H‐ras oncogenes displayed differential sensitivities to cDDP, and the lC50 values of cDDP decreased by approximately 3.0‐, 1.2‐, 2.0‐ and 1.5‐fold, respectively. Over‐expression of rnyc, but not ras, srcor mos, induced apoptosis in R6 cells. Nevertheless, all oncogenes influenced cellular susceptibility to apoptosis triggered by cDDP as judged by the appearance of condensed chromatin, formation of apoptotic bodies and internucleosomal cleavage of genomic DNA to 180 bp multimers, yielding a “laddered” ectrophoretic profile on agarose gel. There was a correlation between the time‐ and concentration‐dependent enhancement of cDDP‐induced DNAfragmentation and potentiation of cDDP sensitivity by various oncogenes. Oncogenic transformation led to a decrease in expression of the PKCr isoform, and stable transfection of PKCr in R6 cells prevented cDDP‐induced apoptosis and protected cells against cDDP cytotoxicity. Our results indicate that the differential sensitivity of oncogenetransformed cells to cDDP may be associated with the ability of various oncogenes to influence cDDP‐induced apoptosis.

Bibliography

Basu, A., & Cline, J. S. (1995). Oncogenic transformation alters cisplatinâinduced apoptosis in rat embryo fibroblasts. International Journal of Cancer, 63(4), 597â603. Portico.

Authors 2

Alakananda Basu (first)
Jeffrey S. Cline (additional)

References 25 Referenced 29

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{'key': 'e_1_2_1_16_1', 'first-page': '5903', 'article-title': 'Expression of the c‐Ha‐ras oncogene in mouse NIH 3T3 cells induces resistance to cisplatin', 'volume': '51', 'author': 'Isonishi S.', 'year': '1991', 'journal-title': 'Cancer Res.'} / Cancer Res. / Expression of the c‐Ha‐ras oncogene in mouse NIH 3T3 cells induces resistance to cisplatin by Isonishi S. (1991)
{'key': 'e_1_2_1_17_1', 'first-page': '1707', 'article-title': 'Induction of apoptotic DNA fragmentation and cell death in HL‐60 human promyelocytic leukemia cells by pharmacological inhibitors of protein kinase C', 'volume': '54', 'author': 'Jarvis W. D.', 'year': '1994', 'journal-title': 'Cancer Res.'} / Cancer Res. / Induction of apoptotic DNA fragmentation and cell death in HL‐60 human promyelocytic leukemia cells by pharmacological inhibitors of protein kinase C by Jarvis W. D. (1994)
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Dates

Type	When
Created	18 years, 6 months ago (Feb. 19, 2007, 8:11 p.m.)
Deposited	1 year, 10 months ago (Oct. 25, 2023, 10:49 p.m.)
Indexed	3 months, 2 weeks ago (May 18, 2025, 12:03 p.m.)
Issued	29 years, 9 months ago (Nov. 15, 1995)
Published	29 years, 9 months ago (Nov. 15, 1995)
Published Online	19 years, 1 month ago (July 17, 2006)
Published Print	29 years, 9 months ago (Nov. 15, 1995)

Funders 0

None

BibTeX

@article{Basu_1995, title={Oncogenic transformation alters cisplatin‐induced apoptosis in rat embryo fibroblasts}, volume={63}, ISSN={1097-0215}, url={http://dx.doi.org/10.1002/ijc.2910630422}, DOI={10.1002/ijc.2910630422}, number={4}, journal={International Journal of Cancer}, publisher={Wiley}, author={Basu, Alakananda and Cline, Jeffrey S.}, year={1995}, month=nov, pages={597–603} }

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