DOI: 10.1002/ijc.2910600206. Expression of PDGF and PDGF receptors in human astrocytoma operation specimens supports the existence of an autocrine loop

Expression of PDGF and PDGF receptors in human astrocytoma operation specimens supports the existence of an autocrine loop

10.1002/ijc.2910600206

Crossref journal-article

Wiley

International Journal of Cancer (311)

Abstract

AbstractEstablished cell lines derived from human malignant astrocytomas typically express a combination of platelet‐derived growth factor (PDGF) and PDGF receptor which could form an autocrine loop. In this study, we screened for the essential components of a PDGF autocrine loop in fresh surgical isolates of human astrocytomas, using in situ hybridization and immunohis‐tochemical techniques. Eight malignant astrocytomas (6 glioblastomas and 2 anaplastic astrocytomas), 5 low‐grade astrocytomas and 4 non‐neoplastic glial specimens (mesial temporal sclerosis) were evaluated. Malignant astrocytomas, and to a lesser extent low‐grade astrocytomas, expressed more PDGF‐A and PDGF‐B than non‐neoplastic glia. PDGF‐α‐receptor expression was elevated both in malignant and in iow‐grade astrocytomas. These data support the argument that PDGF autocrine loops contribute to the unregulated growth of human astrocytomas. Expression of PDGF and PDGF receptor in low‐grade astrocytomas suggests that activation of PDGF autocrine loops may be an early event in the pathogenesis of malignant astrocytomas. © 1995 Wiley‐Liss, Inc.

Bibliography

Guha, A., Dashner, K., Mc Black, P. L., Wagner, J. A., & Stiles, C. D. (1995). Expression of PDGF and PDGF receptors in human astrocytoma operation specimens supports the existence of an autocrine loop. International Journal of Cancer, 60(2), 168â173. Portico.

Authors 5

Abhijit Guha (first)
Kathleen Dashner (additional)
Peter L. Mc Black (additional)
John A. Wagner (additional)
Charles D. Stiles (additional)

References 18 Referenced 224

{'key': 'e_1_2_1_2_1', 'volume-title': 'Current protocols in molecular biology. in situ hybridization', 'author': 'Ausbel F.', 'year': '1989'} / Current protocols in molecular biology. in situ hybridization by Ausbel F. (1989)
10.1227/00006123-198109000-00020
10.1038/328621a0
{'key': 'e_1_2_1_5_1', 'first-page': '357', 'volume-title': 'Viral oncology', 'author': 'Deinhardt F.', 'year': '1980'} / Viral oncology by Deinhardt F. (1980)
{'key': 'e_1_2_1_6_1', 'first-page': '4550', 'article-title': 'Amplification and/or overexpression of platelet‐derived growth‐factor receptors and epidermal‐growth‐factor receptor in human glial tumors', 'volume': '52', 'author': 'Fleming T.', 'year': '1992', 'journal-title': 'Cancer Res.'} / Cancer Res. / Amplification and/or overexpression of platelet‐derived growth‐factor receptors and epidermal‐growth‐factor receptor in human glial tumors by Fleming T. (1992)
10.1126/science.2836952
10.1073/pnas.77.11.6611
{'key': 'e_1_2_1_9_1', 'first-page': '3213', 'article-title': 'Platelet‐derived growth factor and its receptors in human glioma tissue: expression of messenger RNA and protein suggests the presence of autocrine and paracrine loops', 'volume': '52', 'author': 'Hermanson M.', 'year': '1992', 'journal-title': 'Cancer Res.'} / Cancer Res. / Platelet‐derived growth factor and its receptors in human glioma tissue: expression of messenger RNA and protein suggests the presence of autocrine and paracrine loops by Hermanson M. (1992)
10.1073/pnas.85.20.7748
{'key': 'e_1_2_1_11_1', 'first-page': '627', 'article-title': 'Amplification of a‐platelet‐derived‐growth‐factor‐receptor gene lacking an exon coding for a portion of the extracellular region in a primary brain tumor of glial origin', 'volume': '7', 'author': 'Kumahl T.', 'year': '1992', 'journal-title': 'Oncogene'} / Oncogene / Amplification of a‐platelet‐derived‐growth‐factor‐receptor gene lacking an exon coding for a portion of the extracellular region in a primary brain tumor of glial origin by Kumahl T. (1992)
10.1128/MCB.6.10.3537
10.1038/313144a0
10.3171/jns.1989.71.6.0826
10.1172/JCI114675
10.1016/S0021-9258(18)55365-5 / J. Biol. Chem. / Differential expression of platelet‐derived‐growthfactor receptors in human malignant‐glioma cell lines by Nistér M. (1991)
10.1016/S0021-9258(18)81860-9 / J. biol. Chem. / Two different sub‐units associate to create isoform‐specific PDGF receptors by Seifert R. (1989)
10.1128/MCB.13.12.7203
10.1083/jcb.107.5.1947

Dates

Type	When
Created	16 years, 11 months ago (Sept. 24, 2008, 10:11 a.m.)
Deposited	1 year, 10 months ago (Oct. 25, 2023, 8:11 p.m.)
Indexed	3 days, 8 hours ago (Aug. 31, 2025, 6:13 a.m.)
Issued	30 years, 7 months ago (Jan. 17, 1995)
Published	30 years, 7 months ago (Jan. 17, 1995)
Published Online	19 years, 1 month ago (July 18, 2006)
Published Print	30 years, 7 months ago (Jan. 17, 1995)

Funders 0

None

BibTeX

@article{Guha_1995, title={Expression of PDGF and PDGF receptors in human astrocytoma operation specimens supports the existence of an autocrine loop}, volume={60}, ISSN={1097-0215}, url={http://dx.doi.org/10.1002/ijc.2910600206}, DOI={10.1002/ijc.2910600206}, number={2}, journal={International Journal of Cancer}, publisher={Wiley}, author={Guha, Abhijit and Dashner, Kathleen and Mc Black, Peter L. and Wagner, John A. and Stiles, Charles D.}, year={1995}, month=jan, pages={168–173} }

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