DOI: 10.1002/ijc.2910560617. Expression of cytokine/growth factors and their receptors in human melanoma and melanocytes

Expression of cytokine/growth factors and their receptors in human melanoma and melanocytes

10.1002/ijc.2910560617

Crossref journal-article

Wiley

International Journal of Cancer (311)

Abstract

AbstractHuman tumors can constitutively express cytokines and growth factors, but the extent of this expression has not been investigated. Using 44 different probes to cytokines, growth factors, and their receptors, we tested 21 melanoma and 5 melanocyte cultures for RNA transcript expression by reverse transcriptase‐polymerase chain reaction. With 30 amplification cycles, expression of the cytokines interleukin (IL)‐1β, IL‐6, leukemia inhibitory factor (LIF), IL‐7, groα, IL‐8 and the p35 chain of IL‐12 was detected in more than 60% of melanomas. Concomitant receptors for IL‐6 and IL‐7 were also detected. IL‐1α, IL‐5, Rantes, IL‐10, interferon (IFN)‐β, tumor‐necrosis factor (TNF)‐α, G‐colony‐stimulating factor (CSF) and GM‐CSF were expressed at lower levels. Melanocytes showed greatly reduced cytokine RNA transcripts, and only groα was consistently detected. No expression of IL‐2, IL‐3, IL‐4, IL‐9, the p40 chain of IL‐12, IFN‐α or IFN‐γ RNA transcripts was detected in melanomas or melanocytes. The growth factors expressed by melanomas and, after further signal amplification, by melanocytes were transforming growth factor (TGF)‐α, epidermal growth factor (EGF), TGF‐β, endothelial‐cell growth factor (ECGF), basic‐fibroblast growth factor (bFGF), nerve growth factor (NGF) and steel. The receptors EGFR, FGFR, NGFRp70 and c‐kit were also expressed by melanomas and melanocytes. These results point to new possible autocrine and paracrine pathways in melanoma biology.

Bibliography

Mattei, S., Colombo, M. P., Melani, C., Silvani, A., Parmiani, G., & Herlyn, M. (1994). Expression of cytokine/growth factors and their receptors in human melanoma and melanocytes. International Journal of Cancer, 56(6), 853â857. Portico.

Authors 6

Stefano Mattei (first)
Mario P. Colombo (additional)
Cecilia Melani (additional)
Anna Silvani (additional)
Giorgio Parmiani (additional)
Meenhard Herlyn (additional)

References 26 Referenced 178

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Dates

Type	When
Created	18 years, 6 months ago (Feb. 19, 2007, 7:10 p.m.)
Deposited	1 year, 10 months ago (Oct. 24, 2023, 12:28 p.m.)
Indexed	2 months, 1 week ago (June 27, 2025, 10:03 a.m.)
Issued	31 years, 5 months ago (March 15, 1994)
Published	31 years, 5 months ago (March 15, 1994)
Published Online	19 years, 1 month ago (July 17, 2006)
Published Print	31 years, 5 months ago (March 15, 1994)

Funders 0

None

BibTeX

@article{Mattei_1994, title={Expression of cytokine/growth factors and their receptors in human melanoma and melanocytes}, volume={56}, ISSN={1097-0215}, url={http://dx.doi.org/10.1002/ijc.2910560617}, DOI={10.1002/ijc.2910560617}, number={6}, journal={International Journal of Cancer}, publisher={Wiley}, author={Mattei, Stefano and Colombo, Mario P. and Melani, Cecilia and Silvani, Anna and Parmiani, Giorgio and Herlyn, Meenhard}, year={1994}, month=mar, pages={853–857} }

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