DOI: 10.1002/ijc.2910540407. Tumor‐suppressor <i>p53</i> gene in hepatitis C and B virus‐associated human hepatocellular carcinoma

Tumor‐suppressor p53 gene in hepatitis C and B virus‐associated human hepatocellular carcinoma

10.1002/ijc.2910540407

Crossref journal-article

Wiley

International Journal of Cancer (311)

Abstract

AbstractAbnormalities of the tumor‐suppressor pS3 gene have been discovered in human hepatocellular carcinoma (HCC). It is unclear, however, whether HCC related to chronic viral hepatitis is associated with p53 gene alterations. In this study, we have examined pS3 abnormalities in HCC associated with hepatitis C and B virus (HCV and HBV) infections. Tissues from 18 HCC patients from several hospitals throughout the United States were collected (9 were HCV‐infected, 5 were HBV‐infected, I was HCV/HBV‐infected, and 3 were non‐virus‐associated). Immunostaining with monoclonal pAb1801 revealed expression of p53 protein in tumor‐cell nuclei in one HCV‐associated HCC, and in no case of HBV‐associated HCC, while the nuclei of adjacent hepatocytes were negative. Using Hae Ill‐digestion of chromosomal DNA, mutations at codon 249 were not found in any of 18 HCC tissues studied. Direct sequencing demonstrated a mutated codon 244 and a wild‐type codon 249 in the conserved regions (exon 5–8) of p53 gene from the tumor tissue with nuclear p53 expression. By reverse‐transcription‐polymer‐ase chain reaction (RT‐PCR), the expression of p53 mRNA was demonstrated in tumor cells from 10 out of 16 HCC tissues. In conclusion, the specific mutation at codon 249 with G to T transversion was not observed in the HCCs associated with HCV or HBV infections. In HBV or non‐virus‐associated HCCs studied, no other p53 gene abnormalities were found. A point mutation at codon 244 with G to A transition of p53 gene was detected in only one of 10 HCV‐associated HCCs, which suggests that p53 mutations may not play a significant role in HCV‐ or HBV‐associated hepatocarcinogenesis.

Bibliography

Carol Shieh, Y. S., Nguyen, C., Vocal, M. V., & Chu, H. (1993). Tumorâsuppressor p53 gene in hepatitis C and B virusâassociated human hepatocellular carcinoma. International Journal of Cancer, 54(4), 558â562. Portico.

Authors 4

Y. S. Carol Shieh (first)
Chau Nguyen (additional)
Maria V. Vocal (additional)
Hoi‐Weng Chu (additional)

References 21 Referenced 28

10.1073/pnas.87.5.1973
10.1038/350429a0
{'key': 'e_1_2_1_4_1', 'first-page': '555', 'article-title': 'Comparative studies on the detection of hepatitis B virus DNA in frozen and paraffin sections by the polymerase chain reaction', 'volume': '4', 'author': 'Chen M.‐L.', 'year': '1991', 'journal-title': 'Mod. Pathoi'} / Mod. Pathoi / Comparative studies on the detection of hepatitis B virus DNA in frozen and paraffin sections by the polymerase chain reaction by Chen M.‐L. (1991)
10.1128/MCB.8.2.531
{'key': 'e_1_2_1_6_1', 'first-page': '1044', 'article-title': 'Low frequency of p53 gene mutation in tumors induced by aflatoxin Bi in nonhuman primates', 'volume': '52', 'author': 'Fujimoto Y.', 'year': '1992', 'journal-title': 'Cancer Res.'} / Cancer Res. / Low frequency of p53 gene mutation in tumors induced by aflatoxin Bi in nonhuman primates by Fujimoto Y. (1992)
{'key': 'e_1_2_1_7_1', 'first-page': '1271', 'article-title': 'Detection of replicative hepatitis C virus sequences in hepatocellular carcinoma', 'volume': '141', 'author': 'Gerber M. A.', 'year': '1992', 'journal-title': 'Amer. J. Pathoi'} / Amer. J. Pathoi / Detection of replicative hepatitis C virus sequences in hepatocellular carcinoma by Gerber M. A. (1992)
{'key': 'e_1_2_1_8_1', 'first-page': '317', 'volume-title': 'Viral hepatitis', 'author': 'Gerber M. A.', 'year': '1983'} / Viral hepatitis by Gerber M. A. (1983)
10.1128/MCB.9.8.3385
10.1038/352764a0
{'key': 'e_1_2_1_11_1', 'first-page': '237', 'article-title': 'Molecular analysis of the p53 alleles in primary hepatocellular carcinomas and cell lines', 'volume': '6', 'author': 'Hosono S.', 'year': '1991', 'journal-title': 'Oncogene'} / Oncogene / Molecular analysis of the p53 alleles in primary hepatocellular carcinomas and cell lines by Hosono S. (1991)
10.1038/350427a0
{'key': 'e_1_2_1_13_1', 'first-page': '253', 'article-title': 'Detection of hepatitis B virus DNA in formalin‐fixed, paraffin‐embedded liver tissue by the polymerase chain reaction', 'volume': '137', 'author': 'Lampertico P.', 'year': '1990', 'journal-title': 'Amer. J. Pathoi'} / Amer. J. Pathoi / Detection of hepatitis B virus DNA in formalin‐fixed, paraffin‐embedded liver tissue by the polymerase chain reaction by Lampertico P. (1990)
10.1038/278261a0
10.1016/0092-8674(79)90293-9
10.1073/pnas.89.15.7262
10.1111/j.1349-7006.1990.tb02605.x
10.1016/0140-6736(91)92236-U
10.1073/pnas.87.17.6547
{'key': 'e_1_2_1_20_1', 'first-page': '6098', 'article-title': 'Mutation of p53 gene in hepatocellular carcinoma in Taiwan', 'volume': '52', 'author': 'Sheu J.‐C.', 'year': '1992', 'journal-title': 'Cancer Res.'} / Cancer Res. / Mutation of p53 gene in hepatocellular carcinoma in Taiwan by Sheu J.‐C. (1992)
{'key': 'e_1_2_1_21_1', 'first-page': '408', 'article-title': 'Detection of hepatitis C virus sequences in liver tissue by the polymerase chain reaction', 'volume': '65', 'author': 'Shieh Y.‐S. C.', 'year': '1991', 'journal-title': 'Lab. Invest.'} / Lab. Invest. / Detection of hepatitis C virus sequences in liver tissue by the polymerase chain reaction by Shieh Y.‐S. C. (1991)
10.1073/pnas.87.17.6791

Dates

Type	When
Created	18 years, 6 months ago (Feb. 19, 2007, 6:09 p.m.)
Deposited	1 year, 10 months ago (Oct. 25, 2023, 9:48 p.m.)
Indexed	1 year, 7 months ago (Jan. 15, 2024, 12:59 a.m.)
Issued	32 years, 2 months ago (June 19, 1993)
Published	32 years, 2 months ago (June 19, 1993)
Published Online	19 years, 1 month ago (July 18, 2006)
Published Print	32 years, 2 months ago (June 19, 1993)

Funders 0

None

BibTeX

@article{Carol_Shieh_1993, title={Tumor‐suppressor p53 gene in hepatitis C and B virus‐associated human hepatocellular carcinoma}, volume={54}, ISSN={1097-0215}, url={http://dx.doi.org/10.1002/ijc.2910540407}, DOI={10.1002/ijc.2910540407}, number={4}, journal={International Journal of Cancer}, publisher={Wiley}, author={Carol Shieh, Y. S. and Nguyen, Chau and Vocal, Maria V. and Chu, Hoi‐Weng}, year={1993}, month=jun, pages={558–562} }

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