DOI: 10.1002/ijc.2910520227. Schedule‐dependent effects of two consecutive, divided, low doses of actinomycin D on translocation of protein B23, inhibition of cell growth and RNA synthesis in hela cells

Schedule‐dependent effects of two consecutive, divided, low doses of actinomycin D on translocation of protein B23, inhibition of cell growth and RNA synthesis in hela cells

10.1002/ijc.2910520227

Crossref journal-article

Wiley

International Journal of Cancer (311)

Abstract

AbstractThe effects of 2 consecutive, divided, low doses of actinomycin‐D (Act‐D) on cellular localization of protein B23, inhibition of cell growth, RNA synthesis and colony formation were studied in HeLa cells. The second dose of Act‐D was administered at various times after removal of the first dose. One short exposure of HeLa cells to Act‐D had previously been shown to induce “resversible” translocation of protein B23, inhibition of cell growth, and RNA synthesis. Relocalization of protein B23 from the nucleoplasm to nucleoli as well as “reversible” inhibition of cell growth and RNA synthesis were still observed in cells that had been treated with a second dose of Act‐D administered as early as 0–2 hr or as late as 30 hr after removal of the first dose of Act‐D. In contrast, no relocalization of protein B23 from the nucleoplasm to nucleoli was observed in cells that had been treated with a second dose of Act‐D administered 9 hr after removal of the first dose. A second exposure to Act‐D, administered 9 hr after removal of the first dose, caused irreversible inhibition of cell growth and RNA synthesis; a significant inhibitory effect on colony formation was also observed. RNA synthesis in HeLa cells after 2 sequential exposures to Act‐D was further analyzed by 1 % agarose gel electrophoresis. There were higher‐molecular‐weight bands above 28S RNA, which may be the 45S and 32S RNA, observed in the controls and in the cells that had been exposed to Act‐D treatment once or in the cells that underwent Act‐D exposure twice, in which the second dose was administered as early as 0–2 hr or as late as 30 hr after removal of the first dose. These high‐molecular‐weight bands were not observed in the cells that underwent Act‐D exposure twice, in which the second dose was administered 9 hr after removal of the first. These results indicated that cells at different stages of inhibition or that have recovered from the first exposure to Act‐D respond differently to the second short Act‐D exposure. © 1992 Wiley‐Liss, Inc.

Bibliography

Yung, B. Y., Chang, F., Bor, A. M., & Lee, E. S. (1992). Scheduleâdependent effects of two consecutive, divided, low doses of actinomycin D on translocation of protein B23, inhibition of cell growth and RNA synthesis in hela cells. International Journal of Cancer, 52(2), 317â322. Portico.

Authors 4

Benjamin Yat‐Ming Yung (first)
Fu‐Jung Chang (additional)
Amy Meei‐Shuu Bor (additional)
Edith Siew‐Teh Lee (additional)

References 20 Referenced 10

{'key': 'e_1_2_1_2_1', 'first-page': '43', 'volume-title': 'The nucleolus', 'author': 'Busch H.', 'year': '1982'} / The nucleolus by Busch H. (1982)
{'key': 'e_1_2_1_3_1', 'first-page': '211', 'volume-title': 'Nucleolus', 'author': 'Busch H.', 'year': '1970'} / Nucleolus by Busch H. (1970)
10.1016/0014-4827(85)90494-X
{'key': 'e_1_2_1_5_1', 'first-page': '3798', 'article-title': 'Nucleolar protein B23 translocation after doxorubicin treatment in murine tumor cells', 'volume': '47', 'author': 'Chan P. K.', 'year': '1987', 'journal-title': 'Cancer Res.'} / Cancer Res. / Nucleolar protein B23 translocation after doxorubicin treatment in murine tumor cells by Chan P. K. (1987)
10.1016/S0079-6603(08)60742-4
10.1002/1097-0142(19880715)62:2<270::AID-CNCR2820620208>3.0.CO;2-B
{'key': 'e_1_2_1_8_1', 'first-page': '150', 'volume-title': 'Molecular cloning laboratory manual', 'author': 'Maniatis T.', 'year': '1982'} / Molecular cloning laboratory manual by Maniatis T. (1982)
10.1016/0014-4827(83)90332-4
10.1002/jcp.1040720311
10.1002/jcp.1040760202
10.1016/S0021-9258(17)33472-5 / J. biol. Chem. / Relative stoichiometry of ribosomal proteins in HeLa cell nucleoli by Philips W. F. (1976)
10.1021/bi00706a026
10.1016/S0021-9258(18)32789-3 / J. biol. Chem. / Region‐Specific initiation of mouse mammary tumor virus RNA synthesis by endogenous RNA polymerase II in preparations of cell nuclei by Stallcup M. R. (1983)
10.1073/pnas.85.5.1417
10.1016/S0022-2836(66)80012-8
{'key': 'e_1_2_1_17_1', 'first-page': '5987', 'article-title': 'Short exposure to actinomycin D induces “reversible” translocation of protein B23 as well as “reversible” inhibition of cell growth and RNA synthesis in HeLa cells', 'volume': '50', 'author': 'Yung B. Y‐M.', 'year': '1990', 'journal-title': 'Cancer Res.'} / Cancer Res. / Short exposure to actinomycin D induces “reversible” translocation of protein B23 as well as “reversible” inhibition of cell growth and RNA synthesis in HeLa cells by Yung B. Y‐M. (1990)
10.1016/0006-2952(85)90387-9
10.1016/0167-4781(85)90002-8
{'key': 'e_1_2_1_20_1', 'first-page': '922', 'article-title': 'Effects of luzopeptins on protein B23 translocation and ribosomal RNA synthesis in HeLa cells', 'volume': '46', 'author': 'Yung B. Y.‐M.', 'year': '1986', 'journal-title': 'Cancer Res.'} / Cancer Res. / Effects of luzopeptins on protein B23 translocation and ribosomal RNA synthesis in HeLa cells by Yung B. Y.‐M. (1986)
10.1016/0304-4165(87)90149-8

Dates

Type	When
Created	18 years, 6 months ago (Feb. 19, 2007, 5:52 p.m.)
Deposited	1 year, 10 months ago (Oct. 23, 2023, 8:37 p.m.)
Indexed	1 year, 10 months ago (Oct. 24, 2023, 6:47 a.m.)
Issued	32 years, 11 months ago (Sept. 9, 1992)
Published	32 years, 11 months ago (Sept. 9, 1992)
Published Online	19 years, 1 month ago (July 18, 2006)
Published Print	32 years, 11 months ago (Sept. 9, 1992)

Funders 0

None

BibTeX

@article{Yung_1992, title={Schedule‐dependent effects of two consecutive, divided, low doses of actinomycin D on translocation of protein B23, inhibition of cell growth and RNA synthesis in hela cells}, volume={52}, ISSN={1097-0215}, url={http://dx.doi.org/10.1002/ijc.2910520227}, DOI={10.1002/ijc.2910520227}, number={2}, journal={International Journal of Cancer}, publisher={Wiley}, author={Yung, Benjamin Yat‐Ming and Chang, Fu‐Jung and Bor, Amy Meei‐Shuu and Lee, Edith Siew‐Teh}, year={1992}, month=sep, pages={317–322} }

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