DOI: 10.1002/ijc.2910420122. Paracrine influence of human breast stromal fibroblasts on breast epithelial cells: Secretion of a polypeptide which stimulates reductive 17β‐oestradiol dehydrogenase activity

Paracrine influence of human breast stromal fibroblasts on breast epithelial cells: Secretion of a polypeptide which stimulates reductive 17β‐oestradiol dehydrogenase activity

10.1002/ijc.2910420122

Crossref journal-article

Wiley

International Journal of Cancer (311)

Abstract

AbstractWe have previously shown that a stroma‐associated paracrine influence may occur in the human breast. In particular, human breast fibroblasts secrete a factor which stimulates reductive 17ß‐oestradiol dehydrogenase (HSD) activity, thereby regulating tissue concentrations of 17ß‐oestradiol. We report here the results of experiments designed to establish the nature of the enzyme activity stimulating factor. In vitro cell culture techniques were used, in which human breast fibroblast‐conditioned medium was used to grow the human breast cancer cell line, MCF‐7, for 6 days, after which the reductive HSD activity of the monolayers was assessed. The fibroblastic reductive HSD stimulating factor was found to be a trypsinsensitive polypeptide. The polypeptide eluted from a Sephadex G‐75 column as a peak corresponding to a molecular weight of about 50 kDa. The polypeptide exerts its effects by altering the Vmax of 2 of the cytosolic forms of HSD within MCF‐7 cells. This is achieved by a protein‐synthesis‐dependent but calmodulin‐independent mechanism. These results provide further evidence of a paracrine effect by stromal tissue within the human breast and have important implications with respect to the aetiology and treatment of breast cancer.

Bibliography

Adams, E. F., Newton, C. J., Tait, G. H., Braunsberg, H., Reed, M. J., & James, V. H. T. (1988). Paracrine influence of human breast stromal fibroblasts on breast epithelial cells: Secretion of a polypeptide which stimulates reductive 17Î²âoestradiol dehydrogenase activity. International Journal of Cancer, 42(1), 119â122. Portico.

Authors 6

Eric F. Adams (first)
C. J. Newton (additional)
G. H. Tait (additional)
H. Braunsberg (additional)
M. J. Reed (additional)
V. H. T. James (additional)

References 17 Referenced 54

Adams E. F. Newton C. J. Braunsberg H. Shaikh N. Ghilchik M. andJames V. H. T. Effects of human breast fibroblasts on growth and 17ß‐estradiol dehydrogenase activity of MCF‐7 cells in culture.Breast Cancer Res. Treat.(1988) (In press). (10.1007/BF01805840)
10.1111/j.1365-2265.1983.tb00051.x
{'key': 'e_1_2_1_4_1', 'first-page': '82', 'article-title': 'Effects of serum and insulin on the sensitivity of the human breast cancer cell line MCF‐7 to estrogens and antiestrogens', 'volume': '41', 'author': 'Butler W. B.', 'year': '1981', 'journal-title': 'Cancer Res.'} / Cancer Res. / Effects of serum and insulin on the sensitivity of the human breast cancer cell line MCF‐7 to estrogens and antiestrogens by Butler W. B. (1981)
{'key': 'e_1_2_1_5_1', 'first-page': '2040', 'article-title': 'Establishment and characterisation of SV40‐transformed human breast epithelial cell lines', 'volume': '42', 'author': 'Chang S. E.', 'year': '1982', 'journal-title': 'Cancer Res.'} / Cancer Res. / Establishment and characterisation of SV40‐transformed human breast epithelial cell lines by Chang S. E. (1982)
{'key': 'e_1_2_1_6_1', 'first-page': '845', 'article-title': 'Growth of normal and neoplastic mouse mammary tissue epithelial cells in primary culture', 'volume': '74', 'author': 'Enami J.', 'year': '1983', 'journal-title': 'Gann'} / Gann / Growth of normal and neoplastic mouse mammary tissue epithelial cells in primary culture by Enami J. (1983)
10.1210/jcem-63-5-1174
{'key': 'e_1_2_1_8_1', 'first-page': '310', 'article-title': 'Mammary fibroblast influence on normal mouse mammary epithelial cell response to estrogenin vitro', 'volume': '46', 'author': 'Haslam S. Z.', 'year': '1986', 'journal-title': 'Cancer Res.'} / Cancer Res. / Mammary fibroblast influence on normal mouse mammary epithelial cell response to estrogenin vitro by Haslam S. Z. (1986)
10.1002/bjs.1800740326
10.1016/0022-4731(86)90309-2
{'key': 'e_1_2_1_11_1', 'first-page': '471', 'volume-title': 'Hormones and cancer', 'author': 'James V. H. T.', 'year': '1980'} / Hormones and cancer by James V. H. T. (1980)
10.1016/S0021-9258(19)52451-6
{'key': 'e_1_2_1_13_1', 'first-page': '1355', 'article-title': 'Augmentation of response of normal mammary epithelial cells to estradiol by mammary stroma', 'volume': '43', 'author': 'McGrath C. M.', 'year': '1983', 'journal-title': 'Cancer Res.'} / Cancer Res. / Augmentation of response of normal mammary epithelial cells to estradiol by mammary stroma by McGrath C. M. (1983)
10.1016/0304-3835(86)90013-3
10.1016/0022-4731(85)90167-0
10.1097/00003081-198103000-00025
{'key': 'e_1_2_1_17_1', 'first-page': '60', 'article-title': 'Multiple forms of oestradiol 17ß‐hydroxysteroid dehydrogenase in human breast tissues', 'volume': '115', 'author': 'Tait G. H.', 'year': '1987', 'journal-title': 'J. Endocr.'} / J. Endocr. / Multiple forms of oestradiol 17ß‐hydroxysteroid dehydrogenase in human breast tissues by Tait G. H. (1987)
{'key': 'e_1_2_1_18_1', 'first-page': '2900', 'article-title': 'Endogenous concentration and subcellular distribution of estrogens in normal and malignant breast tissue', 'volume': '45', 'author': 'van Landeghem A. A. J.', 'year': '1985', 'journal-title': 'Cancer Res.'} / Cancer Res. / Endogenous concentration and subcellular distribution of estrogens in normal and malignant breast tissue by van Landeghem A. A. J. (1985)

Dates

Type	When
Created	18 years, 6 months ago (Feb. 19, 2007, 4:17 p.m.)
Deposited	1 year, 10 months ago (Oct. 22, 2023, 11:47 a.m.)
Indexed	2 months, 2 weeks ago (June 21, 2025, 12:47 a.m.)
Issued	37 years, 1 month ago (July 15, 1988)
Published	37 years, 1 month ago (July 15, 1988)
Published Online	19 years, 1 month ago (July 17, 2006)
Published Print	37 years, 1 month ago (July 15, 1988)

Funders 0

None

BibTeX

@article{Adams_1988, title={Paracrine influence of human breast stromal fibroblasts on breast epithelial cells: Secretion of a polypeptide which stimulates reductive 17β‐oestradiol dehydrogenase activity}, volume={42}, ISSN={1097-0215}, url={http://dx.doi.org/10.1002/ijc.2910420122}, DOI={10.1002/ijc.2910420122}, number={1}, journal={International Journal of Cancer}, publisher={Wiley}, author={Adams, Eric F. and Newton, C. J. and Tait, G. H. and Braunsberg, H. and Reed, M. J. and James, V. H. T.}, year={1988}, month=jul, pages={119–122} }

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