DOI: 10.1002/ijc.2910400319. Cell shape modulation alters glycosylation of a metastatic melanoma cell‐surface antigen

Cell shape modulation alters glycosylation of a metastatic melanoma cell‐surface antigen

10.1002/ijc.2910400319

Crossref journal-article

Wiley

International Journal of Cancer (311)

Abstract

AbstractB16‐F1 melanoma cells cultivated in vitro as spheroids on a non‐adhesive substrate acquire in a reversible fashion an increase in lung colonization in vivo as compared to cells cultured as a monolayer. After neuraminidase treatment of protein blots, the spheroidal cells expressed an increased binding of 125I‐labelled peanut lectin (PNA) to a unique glycoprotein of Mr78,000 (gp78) which after desialylation migrated in SDS‐polyacrylamide gels as an Mr86,000 protein. Antibodies were generated against this glycoprotein purified on PNA‐Sepharose and its expression on the surface of viable B16‐F1 cells was demonstrated. Growth of B16‐F1 melanoma cells in suspension is associated with the altered glycosylation of gp78 which may be related to the increased metastatic ability of these cells. In vitro treatment of B16‐F1 cells with anti‐gp78 Fab fragments prior to their injection into the tail veins of syngeneic mice resulted in a 2‐fold increase in the appearance of tumor lung colonies.

Bibliography

Nabi, I. R., & Raz, A. (1987). Cell shape modulation alters glycosylation of a metastatic melanoma cellâsurface antigen. International Journal of Cancer, 40(3), 396â402. Portico.

Authors 2

Ivan R. Nabi (first)
Avraham Raz (additional)

References 43 Referenced 47

10.1111/j.1768-322X.1985.tb00321.x
{'key': 'e_1_2_1_3_1', 'first-page': '2632', 'article-title': 'Relationship between the organization and synthesis of vimentin and the metastatic capability of B16 melanoma cells', 'volume': '45', 'author': "Ben‐Ze'ev A.", 'year': '1985', 'journal-title': 'Cancer Res.'} / Cancer Res. / Relationship between the organization and synthesis of vimentin and the metastatic capability of B16 melanoma cells by Ben‐Ze'ev A. (1985)
10.1016/S0021-9258(19)70762-5 / J. biol. Chem. / Purification and partial characterization of a murine melanoma‐Associated antigen by Bhavanandan V. P. (1980)
10.1002/ijc.2910260416
10.1038/newbio242148a0
10.1016/0092-8674(85)90084-4
10.1084/jem.157.1.371
10.1016/0304-419X(75)90011-6
10.1038/273345a0
10.1111/j.1432-1033.1982.tb06478.x
10.1073/pnas.81.21.6752
10.1083/jcb.68.3.642
10.1016/0003-2697(85)90080-6
10.1042/bj0890114
10.1016/0304-419X(85)90004-6
10.1083/jcb.55.2.390
10.4049/jimmunol.117.5_Part_1.1482 / J. Immunol. / Cell membrane antigen isolation with the staphylococcal protein A antibody absorbent by Kessler W. S. (1976)
10.1038/227680a0
10.1016/S0021-9258(19)40790-4
{'key': 'e_1_2_1_21_1', 'first-page': '109', 'article-title': 'Characteriztion of BSp73, a spontaneous rat tumor and its in vivo selected variants showing different metastasizing capacities', 'volume': '3', 'author': 'Matzku S.', 'year': '1983', 'journal-title': 'Invas. Metast.'} / Invas. Metast. / Characteriztion of BSp73, a spontaneous rat tumor and its in vivo selected variants showing different metastasizing capacities by Matzku S. (1983)
{'key': 'e_1_2_1_22_1', 'first-page': '5270', 'article-title': 'Implications of endogenous tumor cell lectins as mediators of cellular interactions and lung colonization', 'volume': '46', 'author': 'Meromsky L.', 'year': '1986', 'journal-title': 'Cancer Res.'} / Cancer Res. / Implications of endogenous tumor cell lectins as mediators of cellular interactions and lung colonization by Meromsky L. (1986)
{'key': 'e_1_2_1_23_1', 'first-page': '1339', 'article-title': 'Binding of peanut lectin to breast epithelium, human carcinomas, and a cultured rat mammary stem cell: use of the lectin as a marker of mammary differentiation', 'volume': '63', 'author': 'Newman R. A.', 'year': '1979', 'journal-title': 'J. nat. Cancer Inst.'} / J. nat. Cancer Inst. / Binding of peanut lectin to breast epithelium, human carcinomas, and a cultured rat mammary stem cell: use of the lectin as a marker of mammary differentiation by Newman R. A. (1979)
10.1016/0304-419X(82)90020-8
10.1038/283139a0
10.1002/ijc.2910290619
10.1126/science.6612347
10.1016/S0021-9258(17)44507-8 / J. biol. Chem. / Endogenous lectins from cultured cells. Isolation and characterization of carbohydrate proteins from 3T3 fibroblasis by Roof C. F. (1983)
{'key': 'e_1_2_1_29_1', 'first-page': '313', 'article-title': 'Importance of cell surface carbohydrates in cancer cell adhesion, invasion, and metastasis', 'volume': '2', 'author': 'Schirrmacher V.', 'year': '1982', 'journal-title': 'Invas. Metast.'} / Invas. Metast. / Importance of cell surface carbohydrates in cancer cell adhesion, invasion, and metastasis by Schirrmacher V. (1982)
{'key': 'e_1_2_1_30_1', 'first-page': '4543', 'article-title': 'Cell surface sialomucin and resistance to cell‐Mediated cytotoxicity of rat mammary tumor ascites cells', 'volume': '46', 'author': 'Sherblom A. P.', 'year': '1986', 'journal-title': 'Cancer Res.'} / Cancer Res. / Cell surface sialomucin and resistance to cell‐Mediated cytotoxicity of rat mammary tumor ascites cells by Sherblom A. P. (1986)
{'key': 'e_1_2_1_31_1', 'first-page': '1178', 'article-title': 'Alteration of cell shape, adhesion, and lipid accumulation in human breast cancer cells (t‐47D) by human prolac tin and growth hormone', 'volume': '44', 'author': 'Shiu R. P.', 'year': '1984', 'journal-title': 'Cancer Res.'} / Cancer Res. / Alteration of cell shape, adhesion, and lipid accumulation in human breast cancer cells (t‐47D) by human prolac tin and growth hormone by Shiu R. P. (1984)
10.1016/0092-8674(85)90077-7
10.1016/0304-419X(83)90006-9
10.1126/science.6729450
10.1002/ijc.2910350516
10.1177/28.9.7410818
{'key': 'e_1_2_1_37_1', 'first-page': '1122', 'article-title': 'Enhanced metastastic potential of cloned Lewis lung carcinoma cells treated in vitro with dimethyl sulfoxide', 'volume': '44', 'author': 'Takenaga K.', 'year': '1984', 'journal-title': 'Cancer Res.'} / Cancer Res. / Enhanced metastastic potential of cloned Lewis lung carcinoma cells treated in vitro with dimethyl sulfoxide by Takenaga K. (1984)
10.1016/0005-2736(81)90309-6
10.1073/pnas.80.12.3729
10.1016/0092-8674(85)90203-X
{'key': 'e_1_2_1_41_1', 'first-page': '97', 'article-title': 'Glycopeptide changes and malignant transformation: a possible role for carbohydrate in malignant behavior', 'volume': '516', 'author': 'Warren L.', 'year': '1978', 'journal-title': 'Biochim. biophys. Acta'} / Biochim. biophys. Acta / Glycopeptide changes and malignant transformation: a possible role for carbohydrate in malignant behavior by Warren L. (1978)
10.1016/0092-8674(81)90111-2
10.1073/pnas.72.4.1579
{'key': 'e_1_2_1_44_1', 'first-page': '3918', 'article-title': 'Relationship between morphology, dissemination, migration, and prostaglandin E2 secretion by cloned variants of Lewis lung carcinoma', 'volume': '45', 'author': 'Young R. M.', 'year': '1985', 'journal-title': 'Cancer Res.'} / Cancer Res. / Relationship between morphology, dissemination, migration, and prostaglandin E2 secretion by cloned variants of Lewis lung carcinoma by Young R. M. (1985)

Dates

Type	When
Created	18 years, 6 months ago (Feb. 19, 2007, 4:09 p.m.)
Deposited	1 year, 10 months ago (Oct. 20, 2023, 12:47 p.m.)
Indexed	1 month, 3 weeks ago (June 29, 2025, 8:03 a.m.)
Issued	37 years, 11 months ago (Sept. 15, 1987)
Published	37 years, 11 months ago (Sept. 15, 1987)
Published Online	19 years, 1 month ago (July 17, 2006)
Published Print	37 years, 11 months ago (Sept. 15, 1987)

Funders 0

None

BibTeX

@article{Nabi_1987, title={Cell shape modulation alters glycosylation of a metastatic melanoma cell‐surface antigen}, volume={40}, ISSN={1097-0215}, url={http://dx.doi.org/10.1002/ijc.2910400319}, DOI={10.1002/ijc.2910400319}, number={3}, journal={International Journal of Cancer}, publisher={Wiley}, author={Nabi, Ivan R. and Raz, Avraham}, year={1987}, month=sep, pages={396–402} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        6,
        29
      ]
    ],
    "date-time": "2025-06-29T12:03:18Z",
    "timestamp": 1751198598470
  },
  "reference-count": 43,
  "publisher": "Wiley",
  "issue": "3",
  "license": [
    {
      "start": {
        "date-parts": [
          [
            2006,
            7,
            17
          ]
        ],
        "date-time": "2006-07-17T00:00:00Z",
        "timestamp": 1153094400000
      },
      "content-version": "vor",
      "delay-in-days": 6880,
      "URL": "http://onlinelibrary.wiley.com/termsAndConditions#vor"
    }
  ],
  "content-domain": {
    "domain": [],
    "crossmark-restriction": false
  },
  "published-print": {
    "date-parts": [
      [
        1987,
        9,
        15
      ]
    ]
  },
  "abstract": "<jats:title>Abstract</jats:title><jats:p>B16\u2010F1 melanoma cells cultivated <jats:italic>in vitro</jats:italic> as spheroids on a non\u2010adhesive substrate acquire in a reversible fashion an increase in lung colonization <jats:italic>in vivo</jats:italic> as compared to cells cultured as a monolayer. After neuraminidase treatment of protein blots, the spheroidal cells expressed an increased binding of <jats:sup>125</jats:sup>I\u2010labelled peanut lectin (PNA) to a unique glycoprotein of M<jats:sub>r</jats:sub>78,000 (gp78) which after desialylation migrated in SDS\u2010polyacrylamide gels as an M<jats:sub>r</jats:sub>86,000 protein. Antibodies were generated against this glycoprotein purified on PNA\u2010Sepharose and its expression on the surface of viable B16\u2010F1 cells was demonstrated. Growth of B16\u2010F1 melanoma cells in suspension is associated with the altered glycosylation of gp78 which may be related to the increased metastatic ability of these cells. <jats:italic>In vitro</jats:italic> treatment of B16\u2010F1 cells with anti\u2010gp78 F<jats:sub>ab</jats:sub> fragments prior to their injection into the tail veins of syngeneic mice resulted in a 2\u2010fold increase in the appearance of tumor lung colonies.</jats:p>",
  "DOI": "10.1002/ijc.2910400319",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2007,
        2,
        19
      ]
    ],
    "date-time": "2007-02-19T21:09:48Z",
    "timestamp": 1171919388000
  },
  "page": "396-402",
  "source": "Crossref",
  "is-referenced-by-count": 47,
  "title": "Cell shape modulation alters glycosylation of a metastatic melanoma cell\u2010surface antigen",
  "prefix": "10.1002",
  "volume": "40",
  "author": [
    {
      "given": "Ivan R.",
      "family": "Nabi",
      "sequence": "first",
      "affiliation": []
    },
    {
      "given": "Avraham",
      "family": "Raz",
      "sequence": "additional",
      "affiliation": []
    }
  ],
  "member": "311",
  "published-online": {
    "date-parts": [
      [
        2006,
        7,
        17
      ]
    ]
  },
  "reference": [
    {
      "key": "e_1_2_1_2_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1768-322X.1985.tb00321.x"
    },
    {
      "key": "e_1_2_1_3_1",
      "first-page": "2632",
      "article-title": "Relationship between the organization and synthesis of vimentin and the metastatic capability of B16 melanoma cells",
      "volume": "45",
      "author": "Ben\u2010Ze'ev A.",
      "year": "1985",
      "journal-title": "Cancer Res."
    },
    {
      "key": "e_1_2_1_4_1",
      "doi-asserted-by": "crossref",
      "first-page": "5145",
      "DOI": "10.1016/S0021-9258(19)70762-5",
      "article-title": "Purification and partial characterization of a murine melanoma\u2010Associated antigen",
      "volume": "255",
      "author": "Bhavanandan V. P.",
      "year": "1980",
      "journal-title": "J. biol. Chem."
    },
    {
      "key": "e_1_2_1_5_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/ijc.2910260416"
    },
    {
      "key": "e_1_2_1_6_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/newbio242148a0"
    },
    {
      "key": "e_1_2_1_7_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(85)90084-4"
    },
    {
      "key": "e_1_2_1_8_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1084/jem.157.1.371"
    },
    {
      "key": "e_1_2_1_9_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0304-419X(75)90011-6"
    },
    {
      "key": "e_1_2_1_10_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/273345a0"
    },
    {
      "key": "e_1_2_1_11_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1111/j.1432-1033.1982.tb06478.x"
    },
    {
      "key": "e_1_2_1_12_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.81.21.6752"
    },
    {
      "key": "e_1_2_1_13_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1083/jcb.68.3.642"
    },
    {
      "key": "e_1_2_1_14_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0003-2697(85)90080-6"
    },
    {
      "key": "e_1_2_1_15_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1042/bj0890114"
    },
    {
      "key": "e_1_2_1_16_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0304-419X(85)90004-6"
    },
    {
      "key": "e_1_2_1_17_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1083/jcb.55.2.390"
    },
    {
      "key": "e_1_2_1_18_1",
      "doi-asserted-by": "crossref",
      "first-page": "1482",
      "DOI": "10.4049/jimmunol.117.5_Part_1.1482",
      "article-title": "Cell membrane antigen isolation with the staphylococcal protein A antibody absorbent",
      "volume": "117",
      "author": "Kessler W. S.",
      "year": "1976",
      "journal-title": "J. Immunol."
    },
    {
      "key": "e_1_2_1_19_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/227680a0"
    },
    {
      "key": "e_1_2_1_20_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/S0021-9258(19)40790-4"
    },
    {
      "key": "e_1_2_1_21_1",
      "first-page": "109",
      "article-title": "Characteriztion of BSp73, a spontaneous rat tumor and its in vivo selected variants showing different metastasizing capacities",
      "volume": "3",
      "author": "Matzku S.",
      "year": "1983",
      "journal-title": "Invas. Metast."
    },
    {
      "key": "e_1_2_1_22_1",
      "first-page": "5270",
      "article-title": "Implications of endogenous tumor cell lectins as mediators of cellular interactions and lung colonization",
      "volume": "46",
      "author": "Meromsky L.",
      "year": "1986",
      "journal-title": "Cancer Res."
    },
    {
      "key": "e_1_2_1_23_1",
      "first-page": "1339",
      "article-title": "Binding of peanut lectin to breast epithelium, human carcinomas, and a cultured rat mammary stem cell: use of the lectin as a marker of mammary differentiation",
      "volume": "63",
      "author": "Newman R. A.",
      "year": "1979",
      "journal-title": "J. nat. Cancer Inst."
    },
    {
      "key": "e_1_2_1_24_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0304-419X(82)90020-8"
    },
    {
      "key": "e_1_2_1_25_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/283139a0"
    },
    {
      "key": "e_1_2_1_26_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/ijc.2910290619"
    },
    {
      "key": "e_1_2_1_27_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.6612347"
    },
    {
      "key": "e_1_2_1_28_1",
      "doi-asserted-by": "crossref",
      "first-page": "10657",
      "DOI": "10.1016/S0021-9258(17)44507-8",
      "article-title": "Endogenous lectins from cultured cells. Isolation and characterization of carbohydrate proteins from 3T3 fibroblasis",
      "volume": "258",
      "author": "Roof C. F.",
      "year": "1983",
      "journal-title": "J. biol. Chem."
    },
    {
      "key": "e_1_2_1_29_1",
      "first-page": "313",
      "article-title": "Importance of cell surface carbohydrates in cancer cell adhesion, invasion, and metastasis",
      "volume": "2",
      "author": "Schirrmacher V.",
      "year": "1982",
      "journal-title": "Invas. Metast."
    },
    {
      "key": "e_1_2_1_30_1",
      "first-page": "4543",
      "article-title": "Cell surface sialomucin and resistance to cell\u2010Mediated cytotoxicity of rat mammary tumor ascites cells",
      "volume": "46",
      "author": "Sherblom A. P.",
      "year": "1986",
      "journal-title": "Cancer Res."
    },
    {
      "key": "e_1_2_1_31_1",
      "first-page": "1178",
      "article-title": "Alteration of cell shape, adhesion, and lipid accumulation in human breast cancer cells (t\u201047D) by human prolac tin and growth hormone",
      "volume": "44",
      "author": "Shiu R. P.",
      "year": "1984",
      "journal-title": "Cancer Res."
    },
    {
      "key": "e_1_2_1_32_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(85)90077-7"
    },
    {
      "key": "e_1_2_1_33_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0304-419X(83)90006-9"
    },
    {
      "key": "e_1_2_1_34_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.6729450"
    },
    {
      "key": "e_1_2_1_35_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/ijc.2910350516"
    },
    {
      "key": "e_1_2_1_36_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1177/28.9.7410818"
    },
    {
      "key": "e_1_2_1_37_1",
      "first-page": "1122",
      "article-title": "Enhanced metastastic potential of cloned Lewis lung carcinoma cells treated in vitro with dimethyl sulfoxide",
      "volume": "44",
      "author": "Takenaga K.",
      "year": "1984",
      "journal-title": "Cancer Res."
    },
    {
      "key": "e_1_2_1_38_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0005-2736(81)90309-6"
    },
    {
      "key": "e_1_2_1_39_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.80.12.3729"
    },
    {
      "key": "e_1_2_1_40_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(85)90203-X"
    },
    {
      "key": "e_1_2_1_41_1",
      "first-page": "97",
      "article-title": "Glycopeptide changes and malignant transformation: a possible role for carbohydrate in malignant behavior",
      "volume": "516",
      "author": "Warren L.",
      "year": "1978",
      "journal-title": "Biochim. biophys. Acta"
    },
    {
      "key": "e_1_2_1_42_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1016/0092-8674(81)90111-2"
    },
    {
      "key": "e_1_2_1_43_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.72.4.1579"
    },
    {
      "key": "e_1_2_1_44_1",
      "first-page": "3918",
      "article-title": "Relationship between morphology, dissemination, migration, and prostaglandin E2 secretion by cloned variants of Lewis lung carcinoma",
      "volume": "45",
      "author": "Young R. M.",
      "year": "1985",
      "journal-title": "Cancer Res."
    }
  ],
  "container-title": "International Journal of Cancer",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fijc.2910400319",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "text-mining"
    },
    {
      "URL": "https://onlinelibrary.wiley.com/doi/pdf/10.1002/ijc.2910400319",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2023,
        10,
        20
      ]
    ],
    "date-time": "2023-10-20T16:47:47Z",
    "timestamp": 1697820467000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://onlinelibrary.wiley.com/doi/10.1002/ijc.2910400319"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1987,
        9,
        15
      ]
    ]
  },
  "references-count": 43,
  "journal-issue": {
    "issue": "3",
    "published-print": {
      "date-parts": [
        [
          1987,
          9,
          15
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1002/ijc.2910400319"
  ],
  "URL": "http://dx.doi.org/10.1002/ijc.2910400319",
  "relation": {},
  "ISSN": [
    "0020-7136",
    "1097-0215"
  ],
  "subject": [],
  "container-title-short": "Intl Journal of Cancer",
  "published": {
    "date-parts": [
      [
        1987,
        9,
        15
      ]
    ]
  }
}