DOI: 10.1002/ijc.2910400214. Epidermal growth factor stimulates the synthesis of cell‐attachment proteins in the human breast cancer cell line PMC42

Epidermal growth factor stimulates the synthesis of cell‐attachment proteins in the human breast cancer cell line PMC42

10.1002/ijc.2910400214

Crossref journal-article

Wiley

International Journal of Cancer (311)

Abstract

AbstractThe human breast cancer cell line PMC42 responds to the addition of epidermal growth factor (EGF) by proliferation and increased frequency of attachment of cell‐organoid structures to the culture vessel. Antibodies to fibronectin and laminin reacted strongly, by immunoperoxidase, with the membranes of cells from organoids that became adherent following addition of EGF. This reaction was weak with membranes of cells of non‐adherent organoids in cultures containing EGF and was negative with membranes of cells of free‐floating organoids from cultures without EGF. An increase in biosynthetic labelling with 35S‐methionine was found in cell lysates and supernatants of PMC42 cells cultured in the presence of EGF compared with control cells grown without EGF. One‐dimensional SDS‐polyacrylamide gel electrophoresis and 2‐dimensional NEPHGE‐PAGE of labelled cell proteins showed increased synthesis of several cellular proteins and the appearance in EGF‐treated cells of 2 proteins which were not detected in cells from control cultures lacking EGF. Immunoprecipitation experiments using antibodies to fibronectin and laminin with lysates of 35S‐methionine labelled PMC42 cells cultured with EGF showed strong immunoprecipitation at Mr 200 and 400 with anti‐laminin, and at Mr 200 and 96 with antifibronectin. These immunoprecipitates were blocked specifically by purified laminin or fibronectin, respectively. No immunoprecipitates were detected with these antibodies in lysates from cells grown without EGF. EGF thus stimulates increased adherence of cultured PMC42 cell‐organoid structures together with increased membrane expression of the cell‐adhesive proteins laminin and fibronectin. These effects may play a role in normal development and neoplastic behaviour of breast epithelia.

Bibliography

Thorne, H. J., Jose, D. G., Zhang, H., Dempsey, P. J., & Whitehead, R. H. (1987). Epidermal growth factor stimulates the synthesis of cellâattachment proteins in the human breast cancer cell line PMC42. International Journal of Cancer, 40(2), 207â212. Portico.

Authors 5

Heather J. Thorne (first)
David G. Jose (additional)
Hui‐Ying Zhang (additional)
Peter J. Dempsey (additional)
Robert H. Whitehead (additional)

References 24 Referenced 19

{'key': 'e_1_2_1_2_1', 'first-page': '5076', 'article-title': 'Basement membrane changes in breast cancer detected by immunohistochemical staining for laminin', 'volume': '41', 'author': 'Albrechtsen R.', 'year': '1981', 'journal-title': 'Cancer Res.'} / Cancer Res. / Basement membrane changes in breast cancer detected by immunohistochemical staining for laminin by Albrechtsen R. (1981)
{'key': 'e_1_2_1_3_1', 'first-page': '245', 'article-title': 'Extracellular matrix (eCM) modulates the EGF‐Induced migration of liver epithelial cells in serum free, hormone supplemented medium', 'volume': '21', 'author': 'Bade E. G.', 'year': '1985', 'journal-title': 'In Vitro'} / In Vitro / Extracellular matrix (eCM) modulates the EGF‐Induced migration of liver epithelial cells in serum free, hormone supplemented medium by Bade E. G. (1985)
{'key': 'e_1_2_1_4_1', 'first-page': '140', 'article-title': 'Loss of basement membrane components by invasive tumors, but not by their benign counterparts', 'volume': '49', 'author': 'Barsky S. H.', 'year': '1983', 'journal-title': 'Lab. Invest.'} / Lab. Invest. / Loss of basement membrane components by invasive tumors, but not by their benign counterparts by Barsky S. H. (1983)
10.1126/science.302030
10.1002/eji.1830120309
10.1007/BF02618177
10.3109/00313028209092120
10.1073/pnas.77.1.385
10.1073/pnas.78.6.3901
10.4049/jimmunol.115.6.1617 / J. Immunol. / Rapid isolation of antigens from cells with a staphylococ‐Cal protein A‐Antibody adsorbent: parameters of the interaction of antibody‐Antigen complexes with protein A by Kessler S. W. (1975)
{'key': 'e_1_2_1_12_1', 'first-page': '123', 'volume-title': 'The role of extracellular matrix in development', 'author': 'Kleinman H. K.', 'year': '1984'} / The role of extracellular matrix in development by Kleinman H. K. (1984)
{'key': 'e_1_2_1_13_1', 'first-page': '636', 'article-title': 'Tumour invasion and the extracellular matrix', 'volume': '49', 'author': 'Liotta L. N.', 'year': '1983', 'journal-title': 'Lab. Invest.'} / Lab. Invest. / Tumour invasion and the extracellular matrix by Liotta L. N. (1983)
10.1002/ijc.2910340106
{'key': 'e_1_2_1_15_1', 'first-page': '2361', 'article-title': 'Epidermal growth factor stimulation of human breast cancer cells in culture', 'volume': '40', 'author': 'Osborne C. K.', 'year': '1980', 'journal-title': 'Cancer Res.'} / Cancer Res. / Epidermal growth factor stimulation of human breast cancer cells in culture by Osborne C. K. (1980)
10.1007/BF00509237
{'key': 'e_1_2_1_17_1', 'first-page': '54', 'article-title': 'Stages of neoplastic transformation of human breast tissue as monitored by dissolution of basement membrane components. An immunoperoxidase study', 'volume': '1', 'author': 'Siegal G. P.', 'year': '1981', 'journal-title': 'Invas. Metast.'} / Invas. Metast. / Stages of neoplastic transformation of human breast tissue as monitored by dissolution of basement membrane components. An immunoperoxidase study by Siegal G. P. (1981)
10.1038/264764a0
10.1002/ijc.2910200613
{'key': 'e_1_2_1_20_1', 'first-page': '2265', 'article-title': 'Role of laminin in the attachment and metastasis of murine tumour cells', 'volume': '42', 'author': 'Terranova V. P.', 'year': '1982', 'journal-title': 'Cancer Res.'} / Cancer Res. / Role of laminin in the attachment and metastasis of murine tumour cells by Terranova V. P. (1982)
{'key': 'e_1_2_1_21_1', 'first-page': '119', 'volume-title': 'Immunochemistry of the extracellular matrix', 'author': 'Timpl R.', 'year': '1982'} / Immunochemistry of the extracellular matrix by Timpl R. (1982)
{'key': 'e_1_2_1_22_1', 'first-page': '1457', 'article-title': 'Stimulation of mammary carcinoma cell proliferation by epidermal growth factor in vitro', 'volume': '29', 'author': 'Turkington R. W.', 'year': '1969', 'journal-title': 'Cancer Res.'} / Cancer Res. / Stimulation of mammary carcinoma cell proliferation by epidermal growth factor in vitro by Turkington R. W. (1969)
{'key': 'e_1_2_1_23_1', 'first-page': '649', 'article-title': 'A new human breast carcinoma cell line (pMC42) with stem cell characteristics. I. Morphological characterization', 'volume': '70', 'author': 'Whitehead R. H.', 'year': '1983', 'journal-title': 'J. nat. Cancer Inst.'} / J. nat. Cancer Inst. / A new human breast carcinoma cell line (pMC42) with stem cell characteristics. I. Morphological characterization by Whitehead R. H. (1983)
{'key': 'e_1_2_1_24_1', 'first-page': '1193', 'article-title': 'A new human breast cancer cell line (pMC42) with stem cell characteristics. II. Characterization of cells growing as organoids', 'volume': '71', 'author': 'Whitehead R. H.', 'year': '1983', 'journal-title': 'J. nat. Cancer Inst.'} / J. nat. Cancer Inst. / A new human breast cancer cell line (pMC42) with stem cell characteristics. II. Characterization of cells growing as organoids by Whitehead R. H. (1983)
{'key': 'e_1_2_1_25_1', 'first-page': '643', 'article-title': 'A new human breast cancer cell line (pMC42) with stem cell characteristics. III. Hormone receptor status and responsiveness', 'volume': '73', 'author': 'Whitehead R. H.', 'year': '1984', 'journal-title': 'J. nat. Cancer Inst.'} / J. nat. Cancer Inst. / A new human breast cancer cell line (pMC42) with stem cell characteristics. III. Hormone receptor status and responsiveness by Whitehead R. H. (1984)

Dates

Type	When
Created	18 years, 6 months ago (Feb. 19, 2007, 4:10 p.m.)
Deposited	1 year, 10 months ago (Oct. 21, 2023, 10:32 a.m.)
Indexed	1 year, 1 month ago (July 12, 2024, 5:09 a.m.)
Issued	38 years ago (Aug. 15, 1987)
Published	38 years ago (Aug. 15, 1987)
Published Online	19 years, 1 month ago (July 17, 2006)
Published Print	38 years ago (Aug. 15, 1987)

Funders 0

None

BibTeX

@article{Thorne_1987, title={Epidermal growth factor stimulates the synthesis of cell‐attachment proteins in the human breast cancer cell line PMC42}, volume={40}, ISSN={1097-0215}, url={http://dx.doi.org/10.1002/ijc.2910400214}, DOI={10.1002/ijc.2910400214}, number={2}, journal={International Journal of Cancer}, publisher={Wiley}, author={Thorne, Heather J. and Jose, David G. and Zhang, Hui‐Ying and Dempsey, Peter J. and Whitehead, Robert H.}, year={1987}, month=aug, pages={207–212} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2024,
        7,
        12
      ]
    ],
    "date-time": "2024-07-12T09:09:50Z",
    "timestamp": 1720775390000
  },
  "reference-count": 24,
  "publisher": "Wiley",
  "issue": "2",
  "license": [
    {
      "start": {
        "date-parts": [
          [
            2006,
            7,
            17
          ]
        ],
        "date-time": "2006-07-17T00:00:00Z",
        "timestamp": 1153094400000
      },
      "content-version": "vor",
      "delay-in-days": 6911,
      "URL": "http://onlinelibrary.wiley.com/termsAndConditions#vor"
    }
  ],
  "content-domain": {
    "domain": [],
    "crossmark-restriction": false
  },
  "published-print": {
    "date-parts": [
      [
        1987,
        8,
        15
      ]
    ]
  },
  "abstract": "<jats:title>Abstract</jats:title><jats:p>The human breast cancer cell line PMC42 responds to the addition of epidermal growth factor (EGF) by proliferation and increased frequency of attachment of cell\u2010organoid structures to the culture vessel. Antibodies to fibronectin and laminin reacted strongly, by immunoperoxidase, with the membranes of cells from organoids that became adherent following addition of EGF. This reaction was weak with membranes of cells of non\u2010adherent organoids in cultures containing EGF and was negative with membranes of cells of free\u2010floating organoids from cultures without EGF. An increase in biosynthetic labelling with <jats:sup>35</jats:sup>S\u2010methionine was found in cell lysates and supernatants of PMC42 cells cultured in the presence of EGF compared with control cells grown without EGF. One\u2010dimensional SDS\u2010polyacrylamide gel electrophoresis and 2\u2010dimensional NEPHGE\u2010PAGE of labelled cell proteins showed increased synthesis of several cellular proteins and the appearance in EGF\u2010treated cells of 2 proteins which were not detected in cells from control cultures lacking EGF. Immunoprecipitation experiments using antibodies to fibronectin and laminin with lysates of <jats:sup>35</jats:sup>S\u2010methionine labelled PMC42 cells cultured with EGF showed strong immunoprecipitation at Mr 200 and 400 with anti\u2010laminin, and at Mr 200 and 96 with antifibronectin. These immunoprecipitates were blocked specifically by purified laminin or fibronectin, respectively. No immunoprecipitates were detected with these antibodies in lysates from cells grown without EGF. EGF thus stimulates increased adherence of cultured PMC42 cell\u2010organoid structures together with increased membrane expression of the cell\u2010adhesive proteins laminin and fibronectin. These effects may play a role in normal development and neoplastic behaviour of breast epithelia.</jats:p>",
  "DOI": "10.1002/ijc.2910400214",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2007,
        2,
        19
      ]
    ],
    "date-time": "2007-02-19T21:10:17Z",
    "timestamp": 1171919417000
  },
  "page": "207-212",
  "source": "Crossref",
  "is-referenced-by-count": 19,
  "title": "Epidermal growth factor stimulates the synthesis of cell\u2010attachment proteins in the human breast cancer cell line PMC42",
  "prefix": "10.1002",
  "volume": "40",
  "author": [
    {
      "given": "Heather J.",
      "family": "Thorne",
      "sequence": "first",
      "affiliation": []
    },
    {
      "given": "David G.",
      "family": "Jose",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Hui\u2010Ying",
      "family": "Zhang",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Peter J.",
      "family": "Dempsey",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Robert H.",
      "family": "Whitehead",
      "sequence": "additional",
      "affiliation": []
    }
  ],
  "member": "311",
  "published-online": {
    "date-parts": [
      [
        2006,
        7,
        17
      ]
    ]
  },
  "reference": [
    {
      "key": "e_1_2_1_2_1",
      "first-page": "5076",
      "article-title": "Basement membrane changes in breast cancer detected by immunohistochemical staining for laminin",
      "volume": "41",
      "author": "Albrechtsen R.",
      "year": "1981",
      "journal-title": "Cancer Res."
    },
    {
      "key": "e_1_2_1_3_1",
      "first-page": "245",
      "article-title": "Extracellular matrix (eCM) modulates the EGF\u2010Induced migration of liver epithelial cells in serum free, hormone supplemented medium",
      "volume": "21",
      "author": "Bade E. G.",
      "year": "1985",
      "journal-title": "In Vitro"
    },
    {
      "key": "e_1_2_1_4_1",
      "first-page": "140",
      "article-title": "Loss of basement membrane components by invasive tumors, but not by their benign counterparts",
      "volume": "49",
      "author": "Barsky S. H.",
      "year": "1983",
      "journal-title": "Lab. Invest."
    },
    {
      "key": "e_1_2_1_5_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1126/science.302030"
    },
    {
      "key": "e_1_2_1_6_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/eji.1830120309"
    },
    {
      "key": "e_1_2_1_7_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1007/BF02618177"
    },
    {
      "key": "e_1_2_1_8_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.3109/00313028209092120"
    },
    {
      "key": "e_1_2_1_9_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.77.1.385"
    },
    {
      "key": "e_1_2_1_10_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1073/pnas.78.6.3901"
    },
    {
      "key": "e_1_2_1_11_1",
      "doi-asserted-by": "crossref",
      "first-page": "1617",
      "DOI": "10.4049/jimmunol.115.6.1617",
      "article-title": "Rapid isolation of antigens from cells with a staphylococ\u2010Cal protein A\u2010Antibody adsorbent: parameters of the interaction of antibody\u2010Antigen complexes with protein A",
      "volume": "115",
      "author": "Kessler S. W.",
      "year": "1975",
      "journal-title": "J. Immunol."
    },
    {
      "key": "e_1_2_1_12_1",
      "first-page": "123",
      "volume-title": "The role of extracellular matrix in development",
      "author": "Kleinman H. K.",
      "year": "1984"
    },
    {
      "key": "e_1_2_1_13_1",
      "first-page": "636",
      "article-title": "Tumour invasion and the extracellular matrix",
      "volume": "49",
      "author": "Liotta L. N.",
      "year": "1983",
      "journal-title": "Lab. Invest."
    },
    {
      "key": "e_1_2_1_14_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/ijc.2910340106"
    },
    {
      "key": "e_1_2_1_15_1",
      "first-page": "2361",
      "article-title": "Epidermal growth factor stimulation of human breast cancer cells in culture",
      "volume": "40",
      "author": "Osborne C. K.",
      "year": "1980",
      "journal-title": "Cancer Res."
    },
    {
      "key": "e_1_2_1_16_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1007/BF00509237"
    },
    {
      "key": "e_1_2_1_17_1",
      "first-page": "54",
      "article-title": "Stages of neoplastic transformation of human breast tissue as monitored by dissolution of basement membrane components. An immunoperoxidase study",
      "volume": "1",
      "author": "Siegal G. P.",
      "year": "1981",
      "journal-title": "Invas. Metast."
    },
    {
      "key": "e_1_2_1_18_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1038/264764a0"
    },
    {
      "key": "e_1_2_1_19_1",
      "doi-asserted-by": "publisher",
      "DOI": "10.1002/ijc.2910200613"
    },
    {
      "key": "e_1_2_1_20_1",
      "first-page": "2265",
      "article-title": "Role of laminin in the attachment and metastasis of murine tumour cells",
      "volume": "42",
      "author": "Terranova V. P.",
      "year": "1982",
      "journal-title": "Cancer Res."
    },
    {
      "key": "e_1_2_1_21_1",
      "first-page": "119",
      "volume-title": "Immunochemistry of the extracellular matrix",
      "author": "Timpl R.",
      "year": "1982"
    },
    {
      "key": "e_1_2_1_22_1",
      "first-page": "1457",
      "article-title": "Stimulation of mammary carcinoma cell proliferation by epidermal growth factor in vitro",
      "volume": "29",
      "author": "Turkington R. W.",
      "year": "1969",
      "journal-title": "Cancer Res."
    },
    {
      "key": "e_1_2_1_23_1",
      "first-page": "649",
      "article-title": "A new human breast carcinoma cell line (pMC42) with stem cell characteristics. I. Morphological characterization",
      "volume": "70",
      "author": "Whitehead R. H.",
      "year": "1983",
      "journal-title": "J. nat. Cancer Inst."
    },
    {
      "key": "e_1_2_1_24_1",
      "first-page": "1193",
      "article-title": "A new human breast cancer cell line (pMC42) with stem cell characteristics. II. Characterization of cells growing as organoids",
      "volume": "71",
      "author": "Whitehead R. H.",
      "year": "1983",
      "journal-title": "J. nat. Cancer Inst."
    },
    {
      "key": "e_1_2_1_25_1",
      "first-page": "643",
      "article-title": "A new human breast cancer cell line (pMC42) with stem cell characteristics. III. Hormone receptor status and responsiveness",
      "volume": "73",
      "author": "Whitehead R. H.",
      "year": "1984",
      "journal-title": "J. nat. Cancer Inst."
    }
  ],
  "container-title": "International Journal of Cancer",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fijc.2910400214",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "text-mining"
    },
    {
      "URL": "https://onlinelibrary.wiley.com/doi/pdf/10.1002/ijc.2910400214",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2023,
        10,
        21
      ]
    ],
    "date-time": "2023-10-21T14:32:02Z",
    "timestamp": 1697898722000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://onlinelibrary.wiley.com/doi/10.1002/ijc.2910400214"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1987,
        8,
        15
      ]
    ]
  },
  "references-count": 24,
  "journal-issue": {
    "issue": "2",
    "published-print": {
      "date-parts": [
        [
          1987,
          8,
          15
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1002/ijc.2910400214"
  ],
  "URL": "http://dx.doi.org/10.1002/ijc.2910400214",
  "relation": {},
  "ISSN": [
    "0020-7136",
    "1097-0215"
  ],
  "subject": [],
  "container-title-short": "Intl Journal of Cancer",
  "published": {
    "date-parts": [
      [
        1987,
        8,
        15
      ]
    ]
  }
}