DOI: 10.1002/ijc.2910350302. Biochemical characterization and cellular localization of a formalin‐resistant melanoma‐associated antigen reacting with monoclonal antibody NKI/C‐3

Biochemical characterization and cellular localization of a formalin‐resistant melanoma‐associated antigen reacting with monoclonal antibody NKI/C‐3

10.1002/ijc.2910350302

Crossref journal-article

Wiley

International Journal of Cancer (311)

Abstract

AbstractA monoclonal antibody (MAb NKI/C‐3) produced against a purified membrane preparation of human melanoma cells reacts preferentially with sections of formaldehyde‐fixed and paraffin‐embedded tissues of melanoma, nevocellular nevi, carcinoids and medullary carcinomas of the thyroid. NKI/C‐3 did not react with basal‐cell carcinoma, brain tissue or brain tumors, and in only 14/196 other tumors was a clear cross‐reactivity observed, e. g. with prostate carcinomas and a minority of primary breast, ovarian, lung and clear‐cell carcinomas. This antibody was used in an immuno‐electron microscopic study for the cellular localization of the antigen. The antigen was dispersed in the cytoplasm of melanoma cells, and more concentrated inside vacuoles and sometimes also on the melanosomes. Occasionally, the antigen was seen on the cell surface.The nature of the antigen was determined in an enzyme immunoassay (EIA). It was found that the antigen is a glycoprotein with a disulfide‐dependent configuration that is essential for recognition by the MAb. The antigen was distributed heterogeneously during gel filtration as well as during SDS‐polyacrylamide gel electrophoresis in the region of 25–110 kd proteins. A purified antigen preparation that was obtained after affinity chromatography on a column of MAb NKI/C‐3 linked to Sepharose 4B contained a carbohydrate:protein ratio of 1:3.5.

Bibliography

Vennegoor, C., Calafat, J., Hageman, Ph., van Buitenen, F., Janssen, H., Kolk, A., & RÃ¼mke, Ph. (1985). Biochemical characterization and cellular localization of a formalinâresistant melanomaâassociated antigen reacting with monoclonal antibody NKI/Câ3. International Journal of Cancer, 35(3), 287â295. Portico.

Authors 7

C. Vennegoor (first)
J. Calafat (additional)
Ph. Hageman (additional)
F. van Buitenen (additional)
H. Janssen (additional)
A. Kolk (additional)
Ph. Rümke (additional)

References 43 Referenced 91

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{'key': 'e_1_2_1_34_1', 'first-page': '583', 'article-title': 'Tissue distribution, molecular profile and shedding of a cytoplasmic antigen identified by the monoclonal antibody 465.12S to human melanoma cells', 'volume': '42', 'author': 'Natali P. G.', 'year': '1982', 'journal-title': 'Cancer Res.'} / Cancer Res. / Tissue distribution, molecular profile and shedding of a cytoplasmic antigen identified by the monoclonal antibody 465.12S to human melanoma cells by Natali P. G. (1982)
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Dates

Type	When
Created	18 years, 6 months ago (Feb. 19, 2007, 3:15 p.m.)
Deposited	1 year, 10 months ago (Oct. 20, 2023, 6:14 p.m.)
Indexed	1 year, 7 months ago (Jan. 12, 2024, 6:45 p.m.)
Issued	40 years, 5 months ago (March 15, 1985)
Published	40 years, 5 months ago (March 15, 1985)
Published Online	19 years, 1 month ago (July 17, 2006)
Published Print	40 years, 5 months ago (March 15, 1985)

Funders 0

None

BibTeX

@article{Vennegoor_1985, title={Biochemical characterization and cellular localization of a formalin‐resistant melanoma‐associated antigen reacting with monoclonal antibody NKI/C‐3}, volume={35}, ISSN={1097-0215}, url={http://dx.doi.org/10.1002/ijc.2910350302}, DOI={10.1002/ijc.2910350302}, number={3}, journal={International Journal of Cancer}, publisher={Wiley}, author={Vennegoor, C. and Calafat, J. and Hageman, Ph. and van Buitenen, F. and Janssen, H. and Kolk, A. and Rümke, Ph.}, year={1985}, month=mar, pages={287–295} }

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