DOI: 10.1002/ijc.2910310104. Production of a mouse monoclonal antibody reactive with a human nuclear antigen associated with cell proliferation

Production of a mouse monoclonal antibody reactive with a human nuclear antigen associated with cell proliferation

10.1002/ijc.2910310104

Crossref journal-article

Wiley

International Journal of Cancer (311)

Abstract

AbstractThe production of a mouse monoclonal antibody, Ki‐67, is described. The Ki‐67 antibody recognized a nuclear antigen present in proliferating cells, but absent in resting cells. Immunostainings with Ki‐67 revealed nuclear reactivity in cells of germinal centres of cortical follicles, cortical thymocytes, neck cells of gastrointestinal mucosa, un‐differentiated spermatogonia and cells of a number of human cell lines. The Ki‐67 antibody did not react with cells known to be in a resting stage, such as lymphocytes, monocytes, parietal cells and Paneth's cells of gastrointestinal mucosa, hepatocytes, renal cells, mature sperm cells, brain cells, etc. Expression of the antigen recognized by Ki‐67 could be induced in peripheral blood lymphocytes after stimulation with phytohaemagglutinin, whereas it disappeared from HL‐60 cells stimulated with phorbol esters to differentiate into mature macrophages in a resting stage. These findings suggest that Ki‐67 is directed against a nuclear antigen associated with cell proliferation. A first series of immunostainings of tumour biopsies indicated that Ki‐67 may be a potent tool for easy and quick evaluation of the proportion of proliferating cells in a tumour.

Bibliography

Gerdes, J., Schwab, U., Lemke, H., & Stein, H. (1983). Production of a mouse monoclonal antibody reactive with a human nuclear antigen associated with cell proliferation. International Journal of Cancer, 31(1), 13â20. Portico.

Authors 4

Johannes Gerdes (first)
Ulrich Schwab (additional)
Hilmar Lemke (additional)
Harald Stein (additional)

References 27 Referenced 2,037

10.1016/0014-2964(66)90052-1
10.1016/0014-2964(68)90054-6
{'key': 'e_1_2_1_4_1', 'first-page': '496', 'volume-title': 'Handbuch der allgemeinen Pathologie', 'author': 'Cottier H.', 'year': '1969'} / Handbuch der allgemeinen Pathologie by Cottier H. (1969)
10.1111/j.1749-6632.1964.tb40692.x
{'key': 'e_1_2_1_6_1', 'first-page': '75', 'article-title': 'Xenoantiserum to human C3 receptors: its preparation and effect on the C3b and C3d receptors of tonsil cells and the C3b receptors of erythrocytes and neutrophils', 'volume': '39', 'author': 'Gerdes J.', 'year': '1980', 'journal-title': 'Immunology'} / Immunology / Xenoantiserum to human C3 receptors: its preparation and effect on the C3b and C3d receptors of tonsil cells and the C3b receptors of erythrocytes and neutrophils by Gerdes J. (1980)
10.1016/0014-2964(74)90148-0
10.1073/pnas.71.3.685
10.1007/BF01632381
10.1111/j.1600-065X.1979.tb00293.x
10.1007/BF02901932 / Virchows Arch. Abt. B Zellpath. / Kombinierte autoradiographisch‐cytophotometrische Unter‐suchungen von Keimzentrumszellen der menschlichen Tonsille by Mitrou P. S. (1969)
10.4049/jimmunol.121.6.2228
{'key': 'e_1_2_1_13_1', 'volume-title': 'Klinische Pathologie des Magen‐Darm‐Traktes', 'author': 'Oehlert W.', 'year': '1978'} / Klinische Pathologie des Magen‐Darm‐Traktes by Oehlert W. (1978)
10.1016/0014-2964(70)90045-9
10.1038/199863a0
10.1002/ijc.2910260605
{'key': 'e_1_2_1_17_1', 'first-page': '1', 'volume-title': 'Handbuch der allgemeinen Pathologie', 'author': 'Schindler R.', 'year': '1969'} / Handbuch der allgemeinen Pathologie by Schindler R. (1969)
10.1093/jnci/58.3.499
10.1016/0022-1759(80)90194-5
10.1177/28.8.7003001
10.1007/BF00405073
10.1002/ijc.2910300411
10.1084/jem.154.6.1899
10.1007/BF01745633
10.1182/blood.V42.4.623.623 / Blood / Kinetics of lymphocytes in chronic lymphocytic leukemia: studies using continuous 3H‐thymidine infusion in two patients by Theml H. (1973)
10.1007/BF01008446
{'key': 'e_1_2_1_27_1', 'first-page': '33', 'volume-title': 'Maligne Lymphome und monoklonale Gammopathien. Hämatologie und Bluttransfusion', 'author': 'Trepel F.', 'year': '1976'} / Maligne Lymphome und monoklonale Gammopathien. Hämatologie und Bluttransfusion by Trepel F. (1976)
Uchanska‐Ziegler B. The human promyelocytic cell line HL‐60 as a model for the study of granulocyte and monocyte differentiationin vitro: selective chemical induction and phenotypic surface analysis by monoclonal antibodies.Ph. D. Thesis Biological Faculty University of Tübingen (1982).

Dates

Type	When
Created	18 years, 6 months ago (Feb. 19, 2007, 2:11 p.m.)
Deposited	1 year, 10 months ago (Oct. 20, 2023, 6:55 a.m.)
Indexed	4 days ago (Aug. 27, 2025, 12:17 p.m.)
Issued	42 years, 7 months ago (Jan. 15, 1983)
Published	42 years, 7 months ago (Jan. 15, 1983)
Published Online	19 years, 1 month ago (July 18, 2006)
Published Print	42 years, 7 months ago (Jan. 15, 1983)

Funders 0

None

BibTeX

@article{Gerdes_1983, title={Production of a mouse monoclonal antibody reactive with a human nuclear antigen associated with cell proliferation}, volume={31}, ISSN={1097-0215}, url={http://dx.doi.org/10.1002/ijc.2910310104}, DOI={10.1002/ijc.2910310104}, number={1}, journal={International Journal of Cancer}, publisher={Wiley}, author={Gerdes, Johannes and Schwab, Ulrich and Lemke, Hilmar and Stein, Harald}, year={1983}, month=jan, pages={13–20} }

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