DOI: 10.1002/ijc.2910290507. Cell‐surface antigens of a clonal human embryonal carcinoma cell line: Morphological and antigenic differentiation in culture

Cell‐surface antigens of a clonal human embryonal carcinoma cell line: Morphological and antigenic differentiation in culture

10.1002/ijc.2910290507

Crossref journal-article

Wiley

International Journal of Cancer (311)

Abstract

AbstractA cloned human embryonal carcinoma (EC) cell line has been derived from a testicular teratocarcinoma, and reproducibly forms EC tumors when injected into athymc (nu/nu) mice. These human EC cells are characterized by a newly described stage‐specific embryonic antigen, SSEA‐3. Unlike murine EC cells, they express major histocompatibility antigens (HLA‐A, B, C and β2‐microglobulin) but do not express the embryonic antigen SSEA‐I. We also report that these cells appear to be capable of differentiation and that this can be induced by initiating cultures at low cell density. Differentiation is marked by the appearance of morphologically distinct cells and by the induction of SSEA‐I, whereas the expression of other antigens, including SSEA‐3, is initially diminished. This well‐characterized system of human EC cells provides a model for the future investigation of other human teratocarcinoma cell lines and for the analysis of cellular differentiation during early human development.

Bibliography

Andrews, P. W., Goodfellow, P. N., Shevinsky, L. H., Bronson, D. L., & Knowles, B. B. (1982). Cellâsurface antigens of a clonal human embryonal carcinoma cell line: Morphological and antigenic differentiation in culture. International Journal of Cancer, 29(5), 523â531. Portico.

Authors 5

Peter W. Andrews (first)
Peter N. Goodfellow (additional)
Lynne H. Shevinsky (additional)
David L. Bronson (additional)
Barbara B. Knowles (additional)

References 49 Referenced 164

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10.1002/ijc.2910250104

Dates

Type	When
Created	18 years, 6 months ago (Feb. 19, 2007, 1:41 p.m.)
Deposited	1 year, 9 months ago (Nov. 12, 2023, 6:30 a.m.)
Indexed	3 weeks, 5 days ago (Aug. 7, 2025, 4:55 a.m.)
Issued	43 years, 3 months ago (May 15, 1982)
Published	43 years, 3 months ago (May 15, 1982)
Published Online	19 years, 1 month ago (July 18, 2006)
Published Print	43 years, 3 months ago (May 15, 1982)

Funders 0

None

BibTeX

@article{Andrews_1982, title={Cell‐surface antigens of a clonal human embryonal carcinoma cell line: Morphological and antigenic differentiation in culture}, volume={29}, ISSN={1097-0215}, url={http://dx.doi.org/10.1002/ijc.2910290507}, DOI={10.1002/ijc.2910290507}, number={5}, journal={International Journal of Cancer}, publisher={Wiley}, author={Andrews, Peter W. and Goodfellow, Peter N. and Shevinsky, Lynne H. and Bronson, David L. and Knowles, Barbara B.}, year={1982}, month=may, pages={523–531} }

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