DOI: 10.1002/ijc.2910190505. Characterization of EBV‐genome negative “null” and “T” cell lines derived from children with acute lymphoblastic leukemia and leukemic transformed non‐Hodgkin lymphoma

Characterization of EBV‐genome negative “null” and “T” cell lines derived from children with acute lymphoblastic leukemia and leukemic transformed non‐Hodgkin lymphoma

10.1002/ijc.2910190505

Crossref journal-article

Wiley

International Journal of Cancer (311)

Abstract

AbstractSixty‐two explants from peripheral blood, bone marrow and cerebral fluid of children with acute lymphoblastic leukemia (ALL) and leukemic transformed non‐Hodgkin lymphoma (NHL) were cultivated for at least 8 weeks. Although lymphatic cells persisted up to 16 weeks in tissue culture, no proliferation was observed in 54 cultures. From the remaining cultures, eight permanently growing cell lines were obtained. Five of these were EBNA (Epstein‐Barr virus‐specific nuclear antigen)‐positive. Three, however, were EBNA‐negative and lacked Epstein‐Barr virus genomes. Two cell lines (KM‐3 and SH‐2) expressed neither B nor T cell characteristics. One line (JM) expressed T cell characteristics and complement receptors. The growing lymphatic cells represented leukemic cells, since the pattern of cytochemical staining and that of membrane receptors of lymphoblasts from the same donor prior to cultivation were identical. All leukemic cell lines were derived from patients in relapse. In contrast, no proliferation of leukemic cells occurred in explants from patients revealing the first manifestation of the disease. These results suggest enhanced growth potential of lymphoblasts resisting antileukemic therapy.

Authors 3

Ulrich Schneider (first)
Hans‐Ulrich Schwenk (additional)
Georg Bornkamm (additional)

References 30 Referenced 522

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Dates

Type	When
Created	18 years, 6 months ago (Feb. 19, 2007, 11:25 a.m.)
Deposited	1 year, 9 months ago (Nov. 12, 2023, 3:08 a.m.)
Indexed	1 week, 1 day ago (Aug. 23, 2025, 1:02 a.m.)
Issued	48 years, 3 months ago (May 15, 1977)
Published	48 years, 3 months ago (May 15, 1977)
Published Online	19 years, 1 month ago (July 17, 2006)
Published Print	48 years, 3 months ago (May 15, 1977)

Funders 0

None

BibTeX

@article{Schneider_1977, title={Characterization of EBV‐genome negative “null” and “T” cell lines derived from children with acute lymphoblastic leukemia and leukemic transformed non‐Hodgkin lymphoma}, volume={19}, ISSN={1097-0215}, url={http://dx.doi.org/10.1002/ijc.2910190505}, DOI={10.1002/ijc.2910190505}, number={5}, journal={International Journal of Cancer}, publisher={Wiley}, author={Schneider, Ulrich and Schwenk, Hans‐Ulrich and Bornkamm, Georg}, year={1977}, month=may, pages={621–626} }

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