DOI: 10.1002/ijc.2910020505. Two established in vitro cell lines from human mesenchymal tumours

Two established in vitro cell lines from human mesenchymal tumours

10.1002/ijc.2910020505

Crossref journal-article

Wiley

International Journal of Cancer (311)

Abstract

AbstractThe development and cultivation of two cell lines from human mesenchymal tumours is described. Lines 2T and 4T differed from normal human fibroblastic cell strains by showing cytological atypia (high nucleocytoplasmic ratio, hyperchromasia and pleo‐morphism), an altered “epithelial‐like” pavement growth pattern, a deficient inhibition of cell division in crowded cultures and a potential for “indefinite” multiplication in vitro. Both lines were chromosomally abnormal in all samples and differed karyo‐typically from any known continuous cell line as well as from each other. Line 2T has retained, at least for 3 months (passage 111–126), a fairly narrow chromosomal mode of 34–38 with two specific marker chromosomes. A high frequency of chromosome breakage was observed in line 2T and extensive chromosome fragmentation, “chromosome pulverization”, occurred. No viruses could, however, be detected in the cultures despite numerous attempts. A control cell strain (2S) was developed from uninvolved skin of one of the tumour patients. Its behaviour followed the general pattern of human diploid cell strains in culture. To our knowledge, the described cell lines are the first derived from human mesenchymal tumours where the availability of a control fibroblast strain will provide us with opportunities to compare autologous normal and neoplastic human mesenchymal cells under in vitro conditions.

Bibliography

Ponten, J., & Saksela, E. (1967). Two established in vitro cell lines from human mesenchymal tumours. International Journal of Cancer, 2(5), 434â447. Portico.

Authors 2

Jan Ponten (first)
Eero Saksela (additional)

References 21 Referenced 264

{'key': 'e_1_2_1_2_1', 'first-page': '556', 'article-title': 'Isolation and long‐term culture of diploid mammalian cell lines', 'volume': '22', 'author': 'Ferguson J.', 'year': '1962', 'journal-title': 'Cancer Res.'} / Cancer Res. / Isolation and long‐term culture of diploid mammalian cell lines by Ferguson J. (1962)
10.3181/00379727-117-29731
10.1016/0042-6822(62)90061-2
10.1016/0014-4827(65)90211-9
10.1016/0014-4827(61)90192-6
10.1073/pnas.50.2.379
10.1016/0014-4827(64)90231-9
Macieira‐Coelho A. Dissociation between inhibition of movement and inhibition of division in RSV transformed fibroblasts.Exp. Cell Res. in press 1967. (10.1016/0014-4827(67)90222-4)
10.1016/0014-4827(66)90373-9
10.1002/1097-0142(196401)17:1<11::AID-CNCR2820170103>3.0.CO;2-E
10.1002/jcp.1030620109
10.1111/j.1601-5223.1965.tb02008.x
Opatija Conference for the characterization and uses of human diploid cell strains. Sept. 1963. Minimum requirements for the selection and use of human diploid cell strains in the production of virus vaccines in:Proceedings of the Conference Publ. Inst. Immunol. Zagreb 709–733(1964).
Pontén J. andMacieira‐Coelho A. Contact inhibition and cell cycle inhibition in cell cultures derived from normal and neoplastic mesenchyme in preparation (1967).
10.1159/000129733
10.1073/pnas.50.2.390
{'key': 'e_1_2_1_18_1', 'first-page': '132', 'article-title': 'Chromosomal damage of human cells induced by the Sendai virus', 'volume': '43', 'author': 'Saksela E.', 'year': '1965', 'journal-title': 'Ann. Med. exp. Fenn.'} / Ann. Med. exp. Fenn. / Chromosomal damage of human cells induced by the Sendai virus by Saksela E. (1965)
10.1016/S0074-7696(08)60556-2
10.1016/0042-6822(64)90253-3
10.4049/jimmunol.88.3.320 / J. Immunol. / Application of a microtechnique to viral serological investigations by Sever J. L. (1962)
{'key': 'e_1_2_1_22_1', 'first-page': '235', 'article-title': 'Culture characteristics of human fibroblasts propagated serially', 'volume': '66', 'author': 'Swim H. E.', 'year': '1957', 'journal-title': 'Amer. J. Hyg.'} / Amer. J. Hyg. / Culture characteristics of human fibroblasts propagated serially by Swim H. E. (1957)

Dates

Type	When
Created	18 years, 6 months ago (Feb. 19, 2007, 8:06 a.m.)
Deposited	1 year, 9 months ago (Nov. 11, 2023, 11:26 p.m.)
Indexed	4 weeks, 1 day ago (Aug. 5, 2025, 8:38 a.m.)
Issued	57 years, 11 months ago (Sept. 15, 1967)
Published	57 years, 11 months ago (Sept. 15, 1967)
Published Online	19 years, 1 month ago (July 18, 2006)
Published Print	57 years, 11 months ago (Sept. 15, 1967)

Funders 0

None

BibTeX

@article{Ponten_1967, title={Two established in vitro cell lines from human mesenchymal tumours}, volume={2}, ISSN={1097-0215}, url={http://dx.doi.org/10.1002/ijc.2910020505}, DOI={10.1002/ijc.2910020505}, number={5}, journal={International Journal of Cancer}, publisher={Wiley}, author={Ponten, Jan and Saksela, Eero}, year={1967}, month=sep, pages={434–447} }

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