DOI: 10.1002/ijc.20452. Anisamide‐targeted stealth liposomes: A potent carrier for targeting doxorubicin to human prostate cancer cells

Anisamide‐targeted stealth liposomes: A potent carrier for targeting doxorubicin to human prostate cancer cells

10.1002/ijc.20452

Crossref journal-article

Wiley

International Journal of Cancer (311)

Abstract

AbstractCertain human malignancies including prostate cancer overexpress sigma receptor, a membrane bound protein that binds haloperidol and various other neuroleptics with high affinity. An anisamide derivatized ligand possesses high affinity for sigma receptors and we hypothesized that its incorporation into the liposomes encapsulating doxorubicin (DOX) can specifically target and deliver the drug to prostate cancer cells that overexpress sigma receptors. A polyethylene glycol phospholipid was derivatized with an anisamide ligand, which was then incorporated into the DOX‐loaded liposome. The resulting anisamide‐conjugated liposomal DOX showed significantly higher toxicity to DU‐145 cells than non‐targeted liposomal DOX, the IC50 being 1.8 μM and 14 μM respectively. The cytotoxicity of the targeted liposomal DOX, however, was significantly blocked by haloperidol, suggesting that the enhanced cytotoxicity was specifically mediated by the sigma receptors. Fluorescence imaging studies after intravenous (i.v.) administration showed that incorporation of anisamide into liposomes significantly improved their accumulation into the tumor. A weekly injection of the targeted liposomal DOX for 4 weeks at a dose of 7.5 mg/kg led to a significant growth inhibition of established DU‐145 tumor in nude mice with minimal toxicity. Free DOX was effective, but associated with significant toxicities. The present study is the first to demonstrate the use of small molecular weight ligand for mediating efficient targeting of liposomal drugs to sigma receptor expressing prostate cancer cells both in vitro and in vivo. © 2004 Wiley‐Liss, Inc.

Bibliography

Banerjee, R., Tyagi, P., Li, S., & Huang, L. (2004). Anisamideâtargeted stealth liposomes: A potent carrier for targeting doxorubicin to human prostate cancer cells. International Journal of Cancer, 112(4), 693â700. Portico.

Authors 4

Rajkumar Banerjee (first)
Pradeep Tyagi (additional)
Song Li (additional)
Leaf Huang (additional)

References 36 Referenced 228

{'key': 'e_1_2_6_2_2', 'first-page': '355', 'article-title': 'Sigma receptors: biology and function', 'volume': '42', 'author': 'Walker JM', 'year': '1990', 'journal-title': 'Pharmacol Rev'} / Pharmacol Rev / Sigma receptors: biology and function by Walker JM (1990)
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10.1016/0922-4106(94)90115-5
{'key': 'e_1_2_6_5_2', 'first-page': '408', 'article-title': 'Sigma‐1 and sigma‐2 receptors are expressed in a wide variety of human and rodent tumor cell lines', 'volume': '55', 'author': 'Vilner BJ', 'year': '1995', 'journal-title': 'Cancer Res'} / Cancer Res / Sigma‐1 and sigma‐2 receptors are expressed in a wide variety of human and rodent tumor cell lines by Vilner BJ (1995)
{'key': 'e_1_2_6_6_2', 'first-page': '2169', 'article-title': 'A malignant melanoma imaging agent: synthesis, characterization, in vitro binding and biodistribution of iodine‐125‐(2‐piperidinylaminoethyl)4‐iodobenzamide', 'volume': '34', 'author': 'John CS', 'year': '1993', 'journal-title': 'J Nucl Med'} / J Nucl Med / A malignant melanoma imaging agent: synthesis, characterization, in vitro binding and biodistribution of iodine‐125‐(2‐piperidinylaminoethyl)4‐iodobenzamide by John CS (1993)
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{'key': 'e_1_2_6_8_2', 'first-page': '3022', 'article-title': 'Synthesis and pharmacological characterization of 4‐[125I]‐N‐(N‐benzylpiperidin‐4‐yl)‐4‐iodobenzamide: a high affinity sigma receptor ligand for potential imaging of breast cancer', 'volume': '55', 'author': 'John CS', 'year': '1995', 'journal-title': 'Cancer Res'} / Cancer Res / Synthesis and pharmacological characterization of 4‐[125I]‐N‐(N‐benzylpiperidin‐4‐yl)‐4‐iodobenzamide: a high affinity sigma receptor ligand for potential imaging of breast cancer by John CS (1995)
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{'key': 'e_1_2_6_10_2', 'first-page': '4578', 'article-title': 'Targeting sigma receptor‐binding benzamides as in vivo diagnostic and therapeutic agents for human prostate tumors', 'volume': '59', 'author': 'John CS', 'year': '1999', 'journal-title': 'Cancer Res'} / Cancer Res / Targeting sigma receptor‐binding benzamides as in vivo diagnostic and therapeutic agents for human prostate tumors by John CS (1999)
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{'key': 'e_1_2_6_15_2', 'first-page': '6214', 'article-title': 'Monoclonal antibody targeting of liposomes to mouse lung in vivo', 'volume': '49', 'author': 'Hughes BJ', 'year': '1989', 'journal-title': 'Cancer Res'} / Cancer Res / Monoclonal antibody targeting of liposomes to mouse lung in vivo by Hughes BJ (1989)
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{'key': 'e_1_2_6_18_2', 'first-page': '3320', 'article-title': 'In vitro and in vivo targeting of immunoliposomal DOX to human B‐cell lymphoma', 'volume': '58', 'author': 'de Menezes DEL', 'year': '1998', 'journal-title': 'Cancer Res'} / Cancer Res / In vitro and in vivo targeting of immunoliposomal DOX to human B‐cell lymphoma by de Menezes DEL (1998)
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{'key': 'e_1_2_6_20_2', 'first-page': '3431', 'article-title': 'pH‐sensitive immunoliposomes as an efficient and target‐specific carrier for antitumor drugs', 'volume': '46', 'author': 'Connor J', 'year': '1986', 'journal-title': 'Cancer Res'} / Cancer Res / pH‐sensitive immunoliposomes as an efficient and target‐specific carrier for antitumor drugs by Connor J (1986)
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{'key': 'e_1_2_6_35_2', 'first-page': '6942', 'article-title': 'Antibody targeting of Doxorubicin‐loaded liposomes suppresses the growth and metastatic spread of established human lung tumor xenografts in severe combined immunodeficient mice', 'volume': '60', 'author': 'Sugano M', 'year': '2000', 'journal-title': 'Cancer Res'} / Cancer Res / Antibody targeting of Doxorubicin‐loaded liposomes suppresses the growth and metastatic spread of established human lung tumor xenografts in severe combined immunodeficient mice by Sugano M (2000)
{'key': 'e_1_2_6_36_2', 'first-page': '1949', 'article-title': 'Nuclear delivery of Doxorubicin via folate‐targeted liposomes with bypass of multidrug‐resistance efflux pump', 'volume': '6', 'author': 'Goren D', 'year': '2000', 'journal-title': 'Clin Cancer Res'} / Clin Cancer Res / Nuclear delivery of Doxorubicin via folate‐targeted liposomes with bypass of multidrug‐resistance efflux pump by Goren D (2000)
10.1182/blood.V100.2.594

Dates

Type	When
Created	21 years, 1 month ago (June 30, 2004, 2:01 p.m.)
Deposited	1 year, 10 months ago (Oct. 13, 2023, 11:28 a.m.)
Indexed	2 weeks, 1 day ago (Aug. 12, 2025, 6:21 p.m.)
Issued	21 years, 1 month ago (June 30, 2004)
Published	21 years, 1 month ago (June 30, 2004)
Published Online	21 years, 1 month ago (June 30, 2004)
Published Print	20 years, 9 months ago (Nov. 20, 2004)

Funders 0

None

BibTeX

@article{Banerjee_2004, title={Anisamide‐targeted stealth liposomes: A potent carrier for targeting doxorubicin to human prostate cancer cells}, volume={112}, ISSN={1097-0215}, url={http://dx.doi.org/10.1002/ijc.20452}, DOI={10.1002/ijc.20452}, number={4}, journal={International Journal of Cancer}, publisher={Wiley}, author={Banerjee, Rajkumar and Tyagi, Pradeep and Li, Song and Huang, Leaf}, year={2004}, month=jun, pages={693–700} }

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