DOI: 10.1002/hep.1840210324. Ex vivo hepatic gene transfer in mouse using a defective herpes simplex virus-1 vector

Ex vivo hepatic gene transfer in mouse using a defective herpes simplex virus-1 vector

10.1002/hep.1840210324

Crossref journal-article

Ovid Technologies (Wolters Kluwer Health)

Hepatology (276)

Abstract

A defective amplicon herpes simplex virus-1 (HSV-1) vector, HSVlac, was used to transfer an E. coli lacZ reporter gene into primary hepatocytes. The lacZ gene was driven by the HSV immediate early (IE) 4/5 promoter. Use of the HSVlac vector resulted in highly efficient gene transfer. Because difficulties in culturing primary hepatocytes impose limitations in ex vivo gene therapy, we sought to determine whether use of the HSVlac vector could simplify gene transfer. Therefore, we incubated HSVlac with primary hepatocytes in suspension and found that the lacZ gene was still transferred with great rapidity and efficiency. To examine lacZ expression in transduced hepatocytes in vivo, we used a mouse hepatocyte transplantation system. In congeneic recipients of primary hepatocytes transduced with HSVlac in suspension, the lacZ gene was expressed in liver and spleen up to 2 weeks. However, survival of transplanted hepatocytes, as well as persistence of HSVlac genome in recipient organs, was demonstrated for up to an 11-week duration of the experiment. These findings suggest that in vivo regulation of the HSV IE4/5 promoter was responsible for the short-term expression of lacZ, which should be overcome by the use of liver-specific promoters. Therefore, our results indicate the feasibility of hepatic gene transfer with a defective HSV-1 vector.

Bibliography

Lu, B., Gupta, S., & Federoff, H. (1995). Ex vivo hepatic gene transfer in mouse using a defective herpes simplex virus-1 vector. Hepatology, 21(3), 752â759.

Authors 3

Bing Lu (first)
Sanjeev Gupta (additional)
Howard Federoff (additional)

References 37 Referenced 5

Hepatocyte-directed somatic gene therapy: a molecular approach for treating genetic disorders. In: ed. Current hepatology. Chicago: Mosby Year Book, 1991; 11: 61–87. / Current hepatology by Gupta (1991)
{'key': '10.1002/hep.1840210324-BIB2', 'first-page': '13', 'volume': '13', 'author': 'Gupta', 'year': '1994', 'journal-title': 'Indian J Gastroenterol'} / Indian J Gastroenterol by Gupta (1994)
10.1126/science.8493530 / Science by Mulligan (1993)
{'key': '10.1002/hep.1840210324-BIB4', 'first-page': '117', 'volume': '6', 'author': 'Friedmann', 'year': '1989', 'journal-title': 'Mol Biol Med'} / Mol Biol Med by Friedmann (1989)
10.1073/pnas.85.9.3014 / Proc Natl Acad Sci U S A by Wilson (1988)
10.1073/pnas.89.19.8981 / Proc Natl Acad Sci U S A by Adams (1992)
10.1073/pnas.88.19.8377 / Proc Natl Acad Sci U S A by Ferry (1991)
10.1055/s-2008-1040402 / Semin Liver Dis by Versland (1992)
Defective interfering herpesvirus. In: , , eds. The human herpes viruses: an interdisciplinary prospective. New York: Elsevier, 1981: 91–120. / The human herpes viruses: an interdisciplinary prospective by Frenkel (1981)
10.1016/0092-8674(82)90035-6 / Cell by Spaete (1982)
. Propagation of foreign DNA sequences linked to a herpes simplex virus origin of replication. In: ed. Eukaryotic viral vector. New York: Cold Spring Harbor Laboratory, 1982: 199–204. / Eukaryotic viral vector by Stow (1982)
{'key': '10.1002/hep.1840210324-BIB12', 'first-page': '497', 'volume': '4', 'author': 'Stow', 'year': '1986', 'journal-title': 'Cancer Cells'} / Cancer Cells by Stow (1986)
10.1126/science.2843986 / Science by Geller (1988)
10.1016/0169-328X(92)90072-J / Mol Brain Res by Johnson (1992)
{'key': '10.1002/hep.1840210324-BIB15', 'first-page': '154.2', 'volume': '16', 'author': 'Federoff', 'year': '1990', 'journal-title': 'Abs Soc Neurosci'} / Abs Soc Neurosci by Federoff (1990)
10.1073/pnas.89.5.1636 / Proc Natl Acad Sci U S A by Federoff (1992)
. The use of HSV-1 vectors to introduce heterologous genes into neurons: implication for gene therapy. In: , eds. Human gene transfer. Vol 219. Paris: John Libbey Eurotext Ltd., 1991: 63–73. / Human gene transfer by Geller (1991)
{'key': '10.1002/hep.1840210324-BIB18', 'first-page': '203', 'volume': '3', 'author': 'Breakefield', 'year': '1991', 'journal-title': 'The New Biologist'} / The New Biologist by Breakefield (1991)
10.1007/BF02890320 / Virchows Arch by Dienes (1984)
10.1002/hep.1840140124 / Hepatology by Gupta (1991)
10.1073/pnas.88.4.1217 / Proc Natl Acad Sci U S A by Ponder (1991)
10.1089/hum.1993.4.3-249 / Hum Gene Ther by Gupta (1993)
{'key': '10.1002/hep.1840210324-BIB23', 'first-page': '3858', 'volume': '42', 'author': 'Nakabayashi', 'year': '1982', 'journal-title': 'Cancer Res'} / Cancer Res by Nakabayashi (1982)
10.1016/0006-8993(77)90770-3 / Brain Res by Greene (1977)
, . Molecular cloning: a laboratory manual. Ed 2. New York: Cold Spring Harbor Laboratory Press, 1989. / Molecular cloning: a laboratory manual by Sambrook (1989)
10.1073/pnas.87.22.8950 / Proc Natl Acad Sci U S A by Geller (1990)
10.1073/pnas.84.1.156 / Proc Natl Acad Sci U S A by Price (1987)
Preparation of isolated rat liver cells. In: ed. Methods in cell biology. Vol 13. New York: Academic Press, 1976: 29–83. / Methods in cell biology by Selglen (1976)
10.1177/4.6.592 / J Histochem Cytochem by Wachstein (1956)
{'key': '10.1002/hep.1840210324-BIB30', 'first-page': '2370', 'volume': '25', 'author': 'Gupta', 'year': '1993', 'journal-title': 'Transplant Proc'} / Transplant Proc by Gupta (1993)
10.1016/0022-2836(84)90427-3 / J Mol Biol by Campbell (1984)
10.1016/0042-6822(92)90695-L / Virology by Daksis (1992)
{'key': '10.1002/hep.1840210324-BIB33', 'first-page': '238', 'volume': '4', 'author': 'Miyanohara', 'year': '1992', 'journal-title': 'The New Biologist'} / The New Biologist by Miyanohara (1992)
10.1128/MMBR.53.3.318-332.1989 / Microbiol Rev by Stevens (1989)
10.1099/0022-1317-67-10-2273 / J Gen Virol by Peat (1986)
, . Extracellular matrix and hormonal regulation of synthesis and abundance of messenger RNAs in cultured liver cells. In: , , , , eds. The liver: biology and pathobiology. Ed 2. New York: Raven Press Limited, 1988: 717–737. / The liver: biology and pathobiology by Reid (1988)
10.1126/science.1722351 / Science by Chowdhury (1991)

Dates

Type	When
Created	18 years, 5 months ago (March 3, 2007, 10:57 p.m.)
Deposited	8 months, 4 weeks ago (Dec. 1, 2024, 6:18 a.m.)
Indexed	8 months, 4 weeks ago (Dec. 2, 2024, 12:03 a.m.)
Issued	30 years, 5 months ago (March 1, 1995)
Published	30 years, 5 months ago (March 1, 1995)
Published Print	30 years, 5 months ago (March 1, 1995)

Funders 0

None

BibTeX

@article{Lu_1995, title={Ex vivo hepatic gene transfer in mouse using a defective herpes simplex virus-1 vector}, volume={21}, ISSN={0270-9139}, url={http://dx.doi.org/10.1002/hep.1840210324}, DOI={10.1002/hep.1840210324}, number={3}, journal={Hepatology}, publisher={Ovid Technologies (Wolters Kluwer Health)}, author={Lu, Bing and Gupta, Sanjeev and Federoff, Howard}, year={1995}, month=mar, pages={752–759} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2024,
        12,
        2
      ]
    ],
    "date-time": "2024-12-02T05:03:00Z",
    "timestamp": 1733115780775,
    "version": "3.30.0"
  },
  "reference-count": 37,
  "publisher": "Ovid Technologies (Wolters Kluwer Health)",
  "issue": "3",
  "license": [
    {
      "start": {
        "date-parts": [
          [
            2015,
            9,
            1
          ]
        ],
        "date-time": "2015-09-01T00:00:00Z",
        "timestamp": 1441065600000
      },
      "content-version": "tdm",
      "delay-in-days": 7489,
      "URL": "http://doi.wiley.com/10.1002/tdm_license_1.1"
    }
  ],
  "content-domain": {
    "domain": [
      "lww.com",
      "ovid.com"
    ],
    "crossmark-restriction": true
  },
  "published-print": {
    "date-parts": [
      [
        1995,
        3
      ]
    ]
  },
  "abstract": "<jats:sec>\n            <jats:title/>\n            <jats:p>A defective amplicon herpes simplex virus-1 (HSV-1) vector, HSVlac, was used to transfer an <jats:italic toggle=\"yes\">E. coli lacZ</jats:italic> reporter gene into primary hepatocytes. The <jats:italic toggle=\"yes\">lacZ</jats:italic> gene was driven by the HSV immediate early (IE) 4/5 promoter. Use of the HSVlac vector resulted in highly efficient gene transfer. Because difficulties in culturing primary hepatocytes impose limitations in <jats:italic toggle=\"yes\">ex vivo</jats:italic> gene therapy, we sought to determine whether use of the HSVlac vector could simplify gene transfer. Therefore, we incubated HSVlac with primary hepatocytes in suspension and found that the <jats:italic toggle=\"yes\">lacZ</jats:italic> gene was still transferred with great rapidity and efficiency. To examine <jats:italic toggle=\"yes\">lacZ</jats:italic> expression in transduced hepatocytes <jats:italic toggle=\"yes\">in vivo</jats:italic>, we used a mouse hepatocyte transplantation system. In congeneic recipients of primary hepatocytes transduced with HSVlac in suspension, the <jats:italic toggle=\"yes\">lacZ</jats:italic> gene was expressed in liver and spleen up to 2 weeks. However, survival of transplanted hepatocytes, as well as persistence of HSVlac genome in recipient organs, was demonstrated for up to an 11-week duration of the experiment. These findings suggest that <jats:italic toggle=\"yes\">in vivo</jats:italic> regulation of the HSV IE4/5 promoter was responsible for the short-term expression of <jats:italic toggle=\"yes\">lacZ</jats:italic>, which should be overcome by the use of liver-specific promoters. Therefore, our results indicate the feasibility of hepatic gene transfer with a defective HSV-1 vector.</jats:p>\n          </jats:sec>",
  "DOI": "10.1002/hep.1840210324",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2007,
        3,
        4
      ]
    ],
    "date-time": "2007-03-04T03:57:54Z",
    "timestamp": 1172980674000
  },
  "page": "752-759",
  "update-policy": "http://dx.doi.org/10.1097/lww.0000000000001000",
  "source": "Crossref",
  "is-referenced-by-count": 5,
  "title": "Ex vivo hepatic gene transfer in mouse using a defective herpes simplex virus-1 vector",
  "prefix": "10.1097",
  "volume": "21",
  "author": [
    {
      "given": "Bing",
      "family": "Lu",
      "sequence": "first",
      "affiliation": []
    },
    {
      "given": "Sanjeev",
      "family": "Gupta",
      "sequence": "additional",
      "affiliation": []
    },
    {
      "given": "Howard",
      "family": "Federoff",
      "sequence": "additional",
      "affiliation": []
    }
  ],
  "member": "276",
  "reference": [
    {
      "key": "10.1002/hep.1840210324-BIB1",
      "first-page": "61",
      "volume-title": "Current hepatology",
      "volume": "11",
      "author": "Gupta",
      "year": "1991",
      "unstructured": "Hepatocyte-directed somatic gene therapy: a molecular approach for treating genetic disorders. In: ed. Current hepatology. Chicago: Mosby Year Book, 1991; 11: 61\u201387."
    },
    {
      "key": "10.1002/hep.1840210324-BIB2",
      "first-page": "13",
      "volume": "13",
      "author": "Gupta",
      "year": "1994",
      "journal-title": "Indian J Gastroenterol"
    },
    {
      "key": "10.1002/hep.1840210324-BIB3",
      "doi-asserted-by": "crossref",
      "first-page": "926",
      "DOI": "10.1126/science.8493530",
      "volume": "260",
      "author": "Mulligan",
      "year": "1993",
      "journal-title": "Science"
    },
    {
      "key": "10.1002/hep.1840210324-BIB4",
      "first-page": "117",
      "volume": "6",
      "author": "Friedmann",
      "year": "1989",
      "journal-title": "Mol Biol Med"
    },
    {
      "key": "10.1002/hep.1840210324-BIB5",
      "doi-asserted-by": "crossref",
      "first-page": "3014",
      "DOI": "10.1073/pnas.85.9.3014",
      "volume": "85",
      "author": "Wilson",
      "year": "1988",
      "journal-title": "Proc Natl Acad Sci U S A"
    },
    {
      "key": "10.1002/hep.1840210324-BIB6",
      "doi-asserted-by": "crossref",
      "first-page": "8981",
      "DOI": "10.1073/pnas.89.19.8981",
      "volume": "89",
      "author": "Adams",
      "year": "1992",
      "journal-title": "Proc Natl Acad Sci U S A"
    },
    {
      "key": "10.1002/hep.1840210324-BIB7",
      "doi-asserted-by": "crossref",
      "first-page": "8377",
      "DOI": "10.1073/pnas.88.19.8377",
      "volume": "88",
      "author": "Ferry",
      "year": "1991",
      "journal-title": "Proc Natl Acad Sci U S A"
    },
    {
      "key": "10.1002/hep.1840210324-BIB8",
      "doi-asserted-by": "crossref",
      "first-page": "332",
      "DOI": "10.1055/s-2008-1040402",
      "volume": "12",
      "author": "Versland",
      "year": "1992",
      "journal-title": "Semin Liver Dis"
    },
    {
      "key": "10.1002/hep.1840210324-BIB9",
      "first-page": "91",
      "volume-title": "The human herpes viruses: an interdisciplinary prospective",
      "author": "Frenkel",
      "year": "1981",
      "unstructured": "Defective interfering herpesvirus. In: , , eds. The human herpes viruses: an interdisciplinary prospective. New York: Elsevier, 1981: 91\u2013120."
    },
    {
      "key": "10.1002/hep.1840210324-BIB10",
      "doi-asserted-by": "crossref",
      "first-page": "295",
      "DOI": "10.1016/0092-8674(82)90035-6",
      "volume": "30",
      "author": "Spaete",
      "year": "1982",
      "journal-title": "Cell"
    },
    {
      "key": "10.1002/hep.1840210324-BIB11",
      "first-page": "199",
      "volume-title": "Eukaryotic viral vector",
      "author": "Stow",
      "year": "1982",
      "unstructured": ". Propagation of foreign DNA sequences linked to a herpes simplex virus origin of replication. In: ed. Eukaryotic viral vector. New York: Cold Spring Harbor Laboratory, 1982: 199\u2013204."
    },
    {
      "key": "10.1002/hep.1840210324-BIB12",
      "first-page": "497",
      "volume": "4",
      "author": "Stow",
      "year": "1986",
      "journal-title": "Cancer Cells"
    },
    {
      "key": "10.1002/hep.1840210324-BIB13",
      "doi-asserted-by": "crossref",
      "first-page": "1667",
      "DOI": "10.1126/science.2843986",
      "volume": "241",
      "author": "Geller",
      "year": "1988",
      "journal-title": "Science"
    },
    {
      "key": "10.1002/hep.1840210324-BIB14",
      "doi-asserted-by": "crossref",
      "first-page": "95",
      "DOI": "10.1016/0169-328X(92)90072-J",
      "volume": "12",
      "author": "Johnson",
      "year": "1992",
      "journal-title": "Mol Brain Res"
    },
    {
      "key": "10.1002/hep.1840210324-BIB15",
      "first-page": "154.2",
      "volume": "16",
      "author": "Federoff",
      "year": "1990",
      "journal-title": "Abs Soc Neurosci"
    },
    {
      "key": "10.1002/hep.1840210324-BIB16",
      "doi-asserted-by": "crossref",
      "first-page": "1636",
      "DOI": "10.1073/pnas.89.5.1636",
      "volume": "89",
      "author": "Federoff",
      "year": "1992",
      "journal-title": "Proc Natl Acad Sci U S A"
    },
    {
      "key": "10.1002/hep.1840210324-BIB17",
      "first-page": "63",
      "volume-title": "Human gene transfer",
      "volume": "219",
      "author": "Geller",
      "year": "1991",
      "unstructured": ". The use of HSV-1 vectors to introduce heterologous genes into neurons: implication for gene therapy. In: , eds. Human gene transfer. Vol 219. Paris: John Libbey Eurotext Ltd., 1991: 63\u201373."
    },
    {
      "key": "10.1002/hep.1840210324-BIB18",
      "first-page": "203",
      "volume": "3",
      "author": "Breakefield",
      "year": "1991",
      "journal-title": "The New Biologist"
    },
    {
      "key": "10.1002/hep.1840210324-BIB19",
      "doi-asserted-by": "crossref",
      "first-page": "321",
      "DOI": "10.1007/BF02890320",
      "volume": "46",
      "author": "Dienes",
      "year": "1984",
      "journal-title": "Virchows Arch"
    },
    {
      "key": "10.1002/hep.1840210324-BIB20",
      "doi-asserted-by": "crossref",
      "first-page": "144",
      "DOI": "10.1002/hep.1840140124",
      "volume": "14",
      "author": "Gupta",
      "year": "1991",
      "journal-title": "Hepatology"
    },
    {
      "key": "10.1002/hep.1840210324-BIB21",
      "doi-asserted-by": "crossref",
      "first-page": "1217",
      "DOI": "10.1073/pnas.88.4.1217",
      "volume": "88",
      "author": "Ponder",
      "year": "1991",
      "journal-title": "Proc Natl Acad Sci U S A"
    },
    {
      "key": "10.1002/hep.1840210324-BIB22",
      "doi-asserted-by": "crossref",
      "first-page": "135",
      "DOI": "10.1089/hum.1993.4.3-249",
      "volume": "4",
      "author": "Gupta",
      "year": "1993",
      "journal-title": "Hum Gene Ther"
    },
    {
      "key": "10.1002/hep.1840210324-BIB23",
      "first-page": "3858",
      "volume": "42",
      "author": "Nakabayashi",
      "year": "1982",
      "journal-title": "Cancer Res"
    },
    {
      "key": "10.1002/hep.1840210324-BIB24",
      "doi-asserted-by": "crossref",
      "first-page": "350",
      "DOI": "10.1016/0006-8993(77)90770-3",
      "volume": "133",
      "author": "Greene",
      "year": "1977",
      "journal-title": "Brain Res"
    },
    {
      "key": "10.1002/hep.1840210324-BIB25",
      "volume-title": "Molecular cloning: a laboratory manual",
      "author": "Sambrook",
      "year": "1989",
      "unstructured": ", . Molecular cloning: a laboratory manual. Ed 2. New York: Cold Spring Harbor Laboratory Press, 1989."
    },
    {
      "key": "10.1002/hep.1840210324-BIB26",
      "doi-asserted-by": "crossref",
      "first-page": "8950",
      "DOI": "10.1073/pnas.87.22.8950",
      "volume": "87",
      "author": "Geller",
      "year": "1990",
      "journal-title": "Proc Natl Acad Sci U S A"
    },
    {
      "key": "10.1002/hep.1840210324-BIB27",
      "doi-asserted-by": "crossref",
      "first-page": "156",
      "DOI": "10.1073/pnas.84.1.156",
      "volume": "84",
      "author": "Price",
      "year": "1987",
      "journal-title": "Proc Natl Acad Sci U S A"
    },
    {
      "key": "10.1002/hep.1840210324-BIB28",
      "first-page": "29",
      "volume-title": "Methods in cell biology",
      "volume": "13",
      "author": "Selglen",
      "year": "1976",
      "unstructured": "Preparation of isolated rat liver cells. In: ed. Methods in cell biology. Vol 13. New York: Academic Press, 1976: 29\u201383."
    },
    {
      "key": "10.1002/hep.1840210324-BIB29",
      "doi-asserted-by": "crossref",
      "first-page": "592",
      "DOI": "10.1177/4.6.592",
      "volume": "4",
      "author": "Wachstein",
      "year": "1956",
      "journal-title": "J Histochem Cytochem"
    },
    {
      "key": "10.1002/hep.1840210324-BIB30",
      "first-page": "2370",
      "volume": "25",
      "author": "Gupta",
      "year": "1993",
      "journal-title": "Transplant Proc"
    },
    {
      "key": "10.1002/hep.1840210324-BIB31",
      "doi-asserted-by": "crossref",
      "first-page": "1",
      "DOI": "10.1016/0022-2836(84)90427-3",
      "volume": "180",
      "author": "Campbell",
      "year": "1984",
      "journal-title": "J Mol Biol"
    },
    {
      "key": "10.1002/hep.1840210324-BIB32",
      "doi-asserted-by": "crossref",
      "first-page": "196",
      "DOI": "10.1016/0042-6822(92)90695-L",
      "volume": "189",
      "author": "Daksis",
      "year": "1992",
      "journal-title": "Virology"
    },
    {
      "key": "10.1002/hep.1840210324-BIB33",
      "first-page": "238",
      "volume": "4",
      "author": "Miyanohara",
      "year": "1992",
      "journal-title": "The New Biologist"
    },
    {
      "key": "10.1002/hep.1840210324-BIB34",
      "doi-asserted-by": "crossref",
      "first-page": "318",
      "DOI": "10.1128/MMBR.53.3.318-332.1989",
      "volume": "53",
      "author": "Stevens",
      "year": "1989",
      "journal-title": "Microbiol Rev"
    },
    {
      "key": "10.1002/hep.1840210324-BIB35",
      "doi-asserted-by": "crossref",
      "first-page": "2273",
      "DOI": "10.1099/0022-1317-67-10-2273",
      "volume": "67",
      "author": "Peat",
      "year": "1986",
      "journal-title": "J Gen Virol"
    },
    {
      "key": "10.1002/hep.1840210324-BIB36",
      "first-page": "717",
      "volume-title": "The liver: biology and pathobiology",
      "author": "Reid",
      "year": "1988",
      "unstructured": ", . Extracellular matrix and hormonal regulation of synthesis and abundance of messenger RNAs in cultured liver cells. In: , , , , eds. The liver: biology and pathobiology. Ed 2. New York: Raven Press Limited, 1988: 717\u2013737."
    },
    {
      "key": "10.1002/hep.1840210324-BIB37",
      "doi-asserted-by": "crossref",
      "first-page": "1802",
      "DOI": "10.1126/science.1722351",
      "volume": "254",
      "author": "Chowdhury",
      "year": "1991",
      "journal-title": "Science"
    }
  ],
  "container-title": "Hepatology",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Fhep.1840210324",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "text-mining"
    },
    {
      "URL": "https://journals.lww.com/01515467-199503000-00023",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2024,
        12,
        1
      ]
    ],
    "date-time": "2024-12-01T11:18:52Z",
    "timestamp": 1733051932000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://journals.lww.com/01515467-199503000-00023"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1995,
        3
      ]
    ]
  },
  "references-count": 37,
  "journal-issue": {
    "issue": "3",
    "published-print": {
      "date-parts": [
        [
          1995
        ]
      ]
    }
  },
  "URL": "http://dx.doi.org/10.1002/hep.1840210324",
  "relation": {},
  "ISSN": [
    "0270-9139"
  ],
  "subject": [],
  "published": {
    "date-parts": [
      [
        1995,
        3
      ]
    ]
  }
}